Complete genome sequence of the archetype bile acid 7α-dehydroxylating bacterium,
            <i>Clostridium scindens</i>
            VPI12708, isolated from human feces, circa 1980

Caicedo, Kelly Yovani Olivos; Fernández-Materán, Francelys V.; Hernandez, Alvaro G.; Daniel, Steven L.; Alves, João M. P.; Ridlon, Jason M.

doi:10.1128/mra.00029-23

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Another important function these microbes may be providing is the hydrolysis of conjugated bile acids (BAs) into secondary BAs. Genomes belonging to Lachnoclostridium, for example, contain a gene cluster required for the multi-step dehydroxylation of BAs into SBAs [ 81 ]. This is significant given that the concentrations of secondary bile acids are significantly lower in dogs with CE compared to healthy dogs [ 8 ] and are hypothesized to inhibit the secretion of inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Microbiome Responses to Oral Fecal Microbiota Transplantation in a Cohort of Domestic Dogs

Rojas,

Entrolezo,

Jarett

et al. 2024

Veterinary Sciences

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Fecal microbiota transplants (FMTs) have been successful at treating digestive and skin conditions in dogs. The degree to which the microbiome is impacted by FMT in a cohort of dogs has not been thoroughly investigated. Using 16S rRNA gene sequencing, we document the changes in the microbiome of fifty-four dogs that took capsules of lyophilized fecal material for their chronic diarrhea, vomiting, or constipation. We found that the relative abundances of five bacterial genera (Butyricicoccus, Faecalibacterium, Fusobacterium, Megamonas, and Sutterella) were higher after FMT than before FMT. Fecal microbiome alpha- and beta-diversity were correlated with kibble and raw food consumption, and prior antibiotic use. On average, 18% of the stool donor’s bacterial amplicon sequence variants (ASVs) engrafted in the FMT recipient, with certain bacterial taxa like Bacteroides spp., Fusobacterium spp., and Lachnoclostridium spp. engrafting more frequently than others. Lastly, analyses indicated that the degree of overlap between the donor bacteria and the community of microbes already established in the FMT recipient likely impacts engraftment. Collectively, our work provides further insight into the microbiome and engraftment dynamics of dogs before and after taking oral FMTs.

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Section: Discussionmentioning

confidence: 99%

Microbiome Responses to Oral Fecal Microbiota Transplantation in a Cohort of Domestic Dogs

Rojas,

Entrolezo,

Jarett

et al. 2024

Veterinary Sciences

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Clostridium scindens: an endocrine keystone species in the mammalian gut

Daniel,

Ridlon

2024

Preprint

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Clostridium scindensis a keystone human gut microbial taxonomic group that, while low in abundance, has a disproportionate effect on bile acid and steroid metabolism in the mammalian gut. Numerous studies indicate that the two most studied strains ofC. scindens(i.e., ATCC 35704 and VPI 12708) are important for a myriad of physiological processes in the host. We focus on both historical and current microbiological and molecular biology work on the Hylemon-Björkhem pathway and the steroid-17,20-desmolase pathway that were first discovered inC. scindens.Our most recent analysis now calls into question whether strains currently defined asC. scindensrepresent two separate taxonomic groups. Future directions include developing genetic tools to further explore the physiological role bile acid and steroid metabolism by strains ofC. scindens, and the causal role of these pathways in host physiology and disease.

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Genome sequences of nine Clostridium scindens strains isolated from human feces

Fernandez-Materan,

Olivos-Caicedo,

Daniel

et al. 2024

Microbiol Resour Announc

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Clostridium scindens is an important member of the gut microbiome. Strains of C. scindens are model organisms for bile acid and steroid metabolism studies. The genome sequences for nine C. scindens strains isolated from human feces are reported. Genomes ranged from 3,403,497 to 4,318,168 bp, 46.5% to 48% G+C content, and 3,386 to 4,137 protein-coding total genes.

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Complete genome sequence of the archetype bile acid 7α-dehydroxylating bacterium, Clostridium scindens VPI12708, isolated from human feces, circa 1980

Cited by 3 publications

References 20 publications

Microbiome Responses to Oral Fecal Microbiota Transplantation in a Cohort of Domestic Dogs

Microbiome Responses to Oral Fecal Microbiota Transplantation in a Cohort of Domestic Dogs

Clostridium scindens: an endocrine keystone species in the mammalian gut

Genome sequences of nine Clostridium scindens strains isolated from human feces

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