Complete Genome Sequence of the Novel Klebsiella pneumoniae Phage Marfa

Harb, Laith; Boeckman, Justin; Newkirk, Heather; Liu, Mei; Gill, Jason J.; Ramsey, Jolene

doi:10.1128/mra.00748-19

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…Globally, K. pneumoniae phages have been isolated from various sources, including sewage [ 18 ], ponds [ 19 ], rivers [ 20 ], seas [ 21 ], farm wastewater [ 22 ], water troughs [ 23 ], animal feces [ 24 ], and clinical samples [ 25 , 26 ], with sewage from hospitals being the most common source [ 27 – 33 ]. The majority of the isolated phages of K. pneumoniae are members of the class Caudoviricetes , which is characterized by being naked (non-enveloped), dsDNA and tailed [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological, biological, and genomic characterization of Klebsiella pneumoniae phage vB_Kpn_ZC2

Fayez

Hakim

Zaki

et al. 2023

Virol J

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Background Bacteriophages (phages) are one of the most promising alternatives to traditional antibiotic therapies, especially against multidrug-resistant bacteria. Klebsiella pneumoniae is considered to be an opportunistic pathogen that can cause life-threatening infections. Thus, this study aims at the characterization of a novel isolated phage vB_Kpn_ZC2 (ZCKP2, for short). Methods The phage ZCKP2 was isolated from sewage water by using the clinical isolate KP/08 as a host strain. The isolated bacteriophage was purified and amplified, followed by testing of its molecular weight using Pulse-Field Gel Electrophoresis (PFGE), transmission electron microscopy, antibacterial activity against a panel of other Klebsiella pneumoniae hosts, stability studies, and whole genome sequencing. Results Phage ZCKP2 belongs morphologically to siphoviruses as indicated from the Transmission Electron Microscopy microgram. The Pulsed Field Gel Electrophoresis and the phage sequencing estimated the phage genome size of 48.2 kbp. Moreover, the absence of lysogeny-related genes, antibiotic resistance genes, and virulence genes in the annotated genome suggests that phage ZCKP2 is safe for therapeutic use. Genome-based taxonomic analysis indicates that phage ZCKP2 represents a new family that has not been formally rated yet. In addition, phage ZCKP2 preserved high stability at different temperatures and pH values (-20 − 70 °C and pH 4 – 9). For the antibacterial activity, phage ZCKP2 maintained consistent clear zones on KP/08 bacteria along with other hosts, in addition to effective bacterial killing over time at different MOIs (0.1, 1, and 10). Also, the genome annotation predicted antibacterial lytic enzymes. Furthermore, the topology of class II holins was predicted in some putative proteins with dual transmembrane domains that contribute significantly to antibacterial activity. Phage ZCKP2 characterization demonstrates safety and efficiency against multidrug-resistant K. pneumoniae, hence ZCKP2 is a good candidate for further in vivo and phage therapy clinical applications.

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Section: Introductionmentioning

confidence: 99%

Morphological, biological, and genomic characterization of Klebsiella pneumoniae phage vB_Kpn_ZC2

Fayez

Hakim

Zaki

et al. 2023

Virol J

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“…There are literature reports [12][13][14] that TEM and SHV types are mainly prevalent in North America and Western Europe, while CTM-M types are prevalent in Eastern Europe, Asia, and South America. In China 15 , CTX-M type is mainly present in South China like Guangdong province, while TEM type is more common in Northern China including Shanxi province.…”

Section: Discussionmentioning

confidence: 99%

Infection status and drug resistance of Klebsiella pneumoniae septicemia in Neonatal Intensive Care Unit

Zhang

et al. 2023

Preprint

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Background To analyze and evaluate the current status, clinical characteristics, and drug resistance of Klebsiella pneumoniaesepsis infection in Neonatal Intensive Care Unit (NICU), providing effective basis for timely and accurate clinical diagnosis and treatment.Methods Statistical analysis was performed on 75 cases of Enterobacteriaceae septicemia in infants admitted to NICU in a special obstetrics and gynecology hospital in Shanghai from January 2020 to June 2022. Based on bacterial identification, isolates were divided into Klebsiella pneumoniae (KP) group (n = 49) and non-KP Enterobacteriaceae group (n = 26). The infection, clinical characteristics, and bacterial resistance of the two groups of infected patients were compared.Results The increase in levels of platelet, procalcitonin, and CRP in the KP group was more significant than that in the non-KP group. The proportion of antimicrobial drug exposure in the KP group is higher than that in the non KP group. The drug resistance ofKP group to ceftazidime, ceftriaxone, cefepime, ampicillin/sulbactam, aztreonam, ciprofloxacin and compound sulfamethoxazole was significantly higher than that of the non-KP group, whereas the drug resistance rate to cefotetan, gentamycin and to bramycin was significantly lower than that of the non-KP group. 38 cases of Klebsiella pneumoniae producing ESBLs were tested for related resistance genes. The results showed that the main resistance types were SHV and TEM, with detection rates of 60.6% and 28.9%.Conclusions The high infection and drug resistance of Klebsiella pneumoniae have become a serious public health issue; Clinicians should pay attention to differential diagnosis, Reasonable selection of antibiotics to reduce the generation of drug-resistant bacteria.

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