Complete Genome Sequence of vB_EcoM-UFV13, a New Bacteriophage Able To Disrupt Trueperella pyogenes Biofilm

Duarte, Viní­cius da Silva; Dias, Roberto Sousa; Kropinski, Andrew M.; Vidigal, Pedro Marcus Pereira; Sousa, Flávia O.; Xavier, André S.; Silva, Cynthia C.; Paula, Sérgio Oliveira de

doi:10.1128/genomea.01292-16

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…Furthermore, the lux S gene regulating the formation of biofilms was also found in the genome sequence [209]. In addition, the presence of a member of the T4virus (vB_EcoM-UFV13) indicates the efficiency in controlling the biofilm formation by T. pyogenes [216]. Moreover, the T. pyogenes genome contains four housekeeping genes ( met G, tuf , gyr A, and fus A).…”

Section: T Pyogenes Genomementioning

confidence: 99%

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Rzewuska

Kwiecień

Chrobak-Chmiel

et al. 2019

IJMS

129

110

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Bacteria from the species Trueperella pyogenes are a part of the biota of skin and mucous membranes of the upper respiratory, gastrointestinal, or urogenital tracts of animals, but also, opportunistic pathogens. T. pyogenes causes a variety of purulent infections, such as metritis, mastitis, pneumonia, and abscesses, which, in livestock breeding, generate significant economic losses. Although this species has been known for a long time, many questions concerning the mechanisms of infection pathogenesis, as well as reservoirs and routes of transmission of bacteria, remain poorly understood. Pyolysin is a major known virulence factor of T. pyogenes that belongs to the family of cholesterol-dependent cytolysins. Its cytolytic activity is associated with transmembrane pore formation. Other putative virulence factors, including neuraminidases, extracellular matrix-binding proteins, fimbriae, and biofilm formation ability, contribute to the adhesion and colonization of the host tissues. However, data about the pathogen–host interactions that may be involved in the development of T. pyogenes infection are still limited. The aim of this review is to present the current knowledge about the pathogenic potential and virulence of T. pyogenes.

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Section: T Pyogenes Genomementioning

confidence: 99%

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Rzewuska

Kwiecień

Chrobak-Chmiel

et al. 2019

IJMS

129

110

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“…It is hypothesized that heterogeneous phages with substantial numbers of VAPGHs can have non-specific hydrolase activity on unrelated hosts when applied at high MOI. In Duarte et al [ 196 ], E. coli phage UFV13 prevented biofilm formation of Trueperella pyogenes . They hypothesized that the phage’s VAPGH disrupted bacterial cell wall components that were required for biofilm buildup.…”

Section: Phage Mechanisms To Overcome the Biofilm Barriermentioning

confidence: 99%

Deconstructing the Phage–Bacterial Biofilm Interaction as a Basis to Establish New Antibiofilm Strategies

Visnapuu

Gucht

Wagemans

et al. 2022

Viruses

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The bacterial biofilm constitutes a complex environment that endows the bacterial community within with an ability to cope with biotic and abiotic stresses. Considering the interaction with bacterial viruses, these biofilms contain intrinsic defense mechanisms that protect against phage predation; these mechanisms are driven by physical, structural, and metabolic properties or governed by environment-induced mutations and bacterial diversity. In this regard, horizontal gene transfer can also be a driver of biofilm diversity and some (pro)phages can function as temporary allies in biofilm development. Conversely, as bacterial predators, phages have developed counter mechanisms to overcome the biofilm barrier. We highlight how these natural systems have previously inspired new antibiofilm design strategies, e.g., by utilizing exopolysaccharide degrading enzymes and peptidoglycan hydrolases. Next, we propose new potential approaches including phage-encoded DNases to target extracellular DNA, as well as phage-mediated inhibitors of cellular communication; these examples illustrate the relevance and importance of research aiming to elucidate novel antibiofilm mechanisms contained within the vast set of unknown ORFs from phages.

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“…[8][9][10] Antimicrobial therapy is the primary measure of treatment for T. pyogenes infections, 11 According to reports, plant secondary metabolites, antimicrobial peptides, and bacteriophages are currently widely studied alternatives to antibiotics and have made significant progress. [12][13][14] Unfortunately, these antibiotic substitutes still need time to follow suit in drug development. However, the therapeutic effectiveness has been attenuated by emerging resistant strains result of the widespread use of antimicrobials in food animals.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Resistance and Virulence Factors of Trueperella pyogenes Isolated from Clinical Bovine Mastitis Cases in China

Zheng,

Yu,

Han

et al. 2024

IDR

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The present study was designed to investigate the resistance determinants and virulence factors of 45 Trueperella pyogenes isolates from clinical bovine mastitis in Hexi Corridor of Gansu, China. Methods: Minimum inhibitory concentrations (MICs) was tested by E-test method. Gene of antimicrobial resistance, virulence integrase and integron gene cassettes were determined by PCR and DNA sequencing. Results: The T. pyogenes isolates exhibited high resistance to streptomycin (88.9%) and tetracycline (64.4%), followed by erythromycin (15.6%) and gentamicin (13.3%). Resistance to streptomycin was most commonly encoded by aadA9 (88.9%); and to tetracycline, by tetW (64.4%). Importantly, all streptomycin-resistant isolates carried aadA9 alone or in combination with aadA1, aadA11 and strA-strB. Similarly, all tetracycline-resistant isolates harbored tetW alone or in combination with tetA33. Meanwhile, ermX was detected in 13.3% isolates, only one erythromycin-resistant isolate was not identified for this gene. Moreover, all T. pyogenes isolates carried class 1 integrons, and 17.8% of them contained gene cassettes, including arrays aadA1-aadB (4.4%), aad A24-dfrA1-ORF1 (2.2%) and aadA1 (2.2%). Furthermore, all tested isolates harbored virulent genes plo and fimA, followed by fimC (88.9%), fimE (86.6%) nanP (75.6%), nanH (40.0%), cbpA (35.6%) and fimG (6.7%). Conclusion:To our knowledge, this is the first report of integron gene cassettes of T. pyogenes isolates from bovine mastitis cases in China. These findings are useful for developing the prevention and the virulence factors of T. pyogenes could be promising candidates for vaccine antigens for bovine mastitis caused by T. pyogenes in China.

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Complete Genome Sequence of vB_EcoM-UFV13, a New Bacteriophage Able To Disrupt Trueperella pyogenes Biofilm

Abstract: vB_EcoM-UFV13, a member of the T4virus genus, shows lytic activity against Escherichia coli and effectiveness in controlling the biofilm formed by Trueperella pyogenes, which qualifies it as a promising component of phage cocktails for mastitis and metritis control.

Cited by 7 publications

References 10 publications

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Deconstructing the Phage–Bacterial Biofilm Interaction as a Basis to Establish New Antibiofilm Strategies

Molecular Characterization of Resistance and Virulence Factors of Trueperella pyogenes Isolated from Clinical Bovine Mastitis Cases in China

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