2005
DOI: 10.1128/aem.71.6.3311-3320.2005
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Complete Genome Sequence of φHSIC, a Pseudotemperate Marine Phage of Listonella pelagia

Abstract: The genome for the marine pseudotemperate member of the Siphoviridae HSIC has been sequenced using a combination of linker amplification library construction, restriction digest library construction, and primer walking. HSIC enters into a pseudolysogenic relationship with its host, Listonella pelagia, characterized by sigmoidal growth curves producing >10 9 cells/ml and >10 11 phage/ml. The genome (37,966 bp; G؉C content, 44%) contained 47 putative open reading frames (ORFs), 17 of which had significant BLASTP… Show more

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Cited by 33 publications
(26 citation statements)
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“…A blue-white band containing the phage was removed (2 ml total volume) and dialysed (Pierce Slide-A-Lyzer 10 K MWCO, Rockford, IL, USA) twice in 1 l buffer (10 mM NaCl, 50 mM Tris-HCl (pH 8), 10 mM MgCl 2 ) to remove CsCl. Phages were concentrated 10 Â (Microcon 30 kD; Millipore, Billerica, MA, USA) and proteins denatured by five freeze-thaw (96 1C) cycles and 12% sodium dodecyl sulphate, then separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, as described by Paul et al (2005). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry peptide mass fingerprint spectra were generated from trypsindigested bands excised from polyacrylamide gel electrophoresis gel (TOPLAB GmbH; Martinsried, Germany).…”
Section: Virion Structural Proteome Analysismentioning
confidence: 99%
“…A blue-white band containing the phage was removed (2 ml total volume) and dialysed (Pierce Slide-A-Lyzer 10 K MWCO, Rockford, IL, USA) twice in 1 l buffer (10 mM NaCl, 50 mM Tris-HCl (pH 8), 10 mM MgCl 2 ) to remove CsCl. Phages were concentrated 10 Â (Microcon 30 kD; Millipore, Billerica, MA, USA) and proteins denatured by five freeze-thaw (96 1C) cycles and 12% sodium dodecyl sulphate, then separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, as described by Paul et al (2005). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry peptide mass fingerprint spectra were generated from trypsindigested bands excised from polyacrylamide gel electrophoresis gel (TOPLAB GmbH; Martinsried, Germany).…”
Section: Virion Structural Proteome Analysismentioning
confidence: 99%
“…on May 10, 2018 by guest http://jvi.asm.org/ rine bacteria (46,56). These marine phages most likely have lytic genes, but they remain unknown due to the bias of genomic databases toward terrestrial and medical phages.…”
Section: Vol 82 2008mentioning
confidence: 99%
“…This results in DNA of different lengths with no specific sequence at the ends of the majority of the packaged phage genomes. These types of tailed phages have circularly permuted genomes; thus, the terminase small subunits are often selected as the arbitrary start points for genomic maps to ease comparison between phages (13,46). This convention was followed when constructing the ⌽HAP-1 genomic map.…”
Section: Vol 82 2008mentioning
confidence: 99%
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