Complete Genome Sequences of Chikungunya Virus Strains Isolated in Mexico: First Detection of Imported and Autochthonous Cases

Díaz-Quiñonez, José Alberto; Ortiz-Alcántara, Joanna María; Fragoso-Fonseca, David Esaú; Garcés-Ayala, Fabiola; Escobar-Escamilla, Noé; Vázquez-Pichardo, Mauricio; Núñez-León, Alma; Torres-Rodríguez, María de la Luz; Torres-Longoria, Belem; López-Martı́nez, Irma; Ruiz-Matus, Cuauhtémoc; Kuri‐Morales, Pablo; Ramírez–González, José Ernesto

doi:10.1128/genomea.00300-15

Cited by 29 publications

(34 citation statements)

References 6 publications

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“…Next, we tested the antiviral effect of tomatidine towards CHIKV strains from other genotypes, namely the originally isolated African (S27) strain from 1953 and a recently isolated Asian CHIKV genotype (99659) in Huh7 cells 31,32 . For both strains, a strong dose-dependent decrease in virus particle production was observed (Fig.…”

Section: Resultsmentioning

confidence: 99%

Tomatidine, a natural steroidal alkaloid shows antiviral activity towards chikungunya virus in vitro

Troost

Mulder

Diosa-Toro

et al. 2020

Sci Rep

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in recent decades, chikungunya virus (cHiKV) has re-emerged, leading to outbreaks of chikungunya fever in Africa, Asia and central and South America. the disease is characterized by a rapid onset febrile illness with (poly)arthralgia, myalgia, rashes, headaches and nausea. In 30 to 40% of the cases, CHIKV infection causes persistent (poly)arthralgia, lasting for months or even years after initial infection. Despite the drastic re-emergence and clinical impact there is no vaccine nor antiviral compound available to prevent or control cHiKV infection. Here, we evaluated the antiviral potential of tomatidine towards cHiKV infection. We demonstrate that tomatidine potently inhibits virus particle production of multiple CHIKV strains. Time-of-addition experiments in Huh7 cells revealed that tomatidine acts at a post-entry step of the virus replication cycle. furthermore, a marked decrease in the number of cHiKV-infected cells was seen, suggesting that tomatidine predominantly acts early in infection yet after virus attachment and cell entry. Antiviral activity was still detected at 24 hours post-infection, indicating that tomatidine controls multiple rounds of cHiKV replication. Solasodine and sarsasapogenin, two structural derivatives of tomatidine, also showed strong albeit less potent antiviral activity towards cHiKV. in conclusion, this study identifies tomatidine as a novel compound to combat CHIKV infection in vitro.

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Section: Resultsmentioning

confidence: 99%

Tomatidine, a natural steroidal alkaloid shows antiviral activity towards chikungunya virus in vitro

Troost

Mulder

Diosa-Toro

et al. 2020

Sci Rep

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“…Nucleotide and amino acid identity among the consensus sequences from these individuals was >99.9%. The MCC phylogeny showed that the Dominican Republic sequences clustered within the Asian lineage together with other Caribbean sequences isolated in 2014 [11, 16]. The most closely related Asian sequences circulated between 2012 and 2013 in Micronesia, the Philippines and China.…”

Section: Supporting Informationmentioning

confidence: 99%

“…albopictus , which itself spread from Asia to many parts of the world since 1985 [7–9]. Then, in 2013, a CHIKV strain belonging to the same lineage that had been circulating in Asia at least since the 1950s (Asian lineage) was introduced into the Americas, first detected on the Caribbean island of St. Martin before spreading through South and Central America, including Mexico [10, 11]. Local transmission was reported in the U.S. in Florida and Texas after the arrival of infected travelers [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Molecular Virologic and Clinical Characteristics of a Chikungunya Fever Outbreak in La Romana, Dominican Republic, 2014

et al. 2016

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Since emerging in Saint Martin in 2013, chikungunya virus (CHIKV), an alphavirus transmitted by the Aedes aegypti mosquito, has infected approximately two million individuals in the Americas, with over 500,000 reported cases in the Dominican Republic (DR). CHIKV-infected patients typically present with a febrile syndrome including polyarthritis/polyarthralgia, and a macropapular rash, similar to those infected with dengue and Zika viruses, and malaria. Nevertheless, many Dominican cases are unconfirmed due to the unavailability and high cost of laboratory testing and the absence of specific treatment for CHIKV infection. To obtain a more accurate representation of chikungunya fever (CHIKF) clinical signs and symptoms, and confirm the viral lineage responsible for the DR CHIKV outbreak, we tested 194 serum samples for CHIKV RNA and IgM antibodies from patients seen in a hospital in La Romana, DR using quantitative RT-PCR and IgM capture ELISA, and performed retrospective chart reviews. RNA and antibodies were detected in 49% and 24.7% of participants, respectively. Sequencing revealed that the CHIKV strain responsible for the La Romana outbreak belonged to the Asian/American lineage and grouped phylogenetically with recent Mexican and Trinidadian isolates. Our study shows that, while CHIKV-infected individuals were infrequently diagnosed with CHIKF, uninfected patients were never falsely diagnosed with CHIKF. Participants testing positive for CHIKV RNA were more likely to present with arthralgia, although it was reported in just 20.0% of CHIKF+ individuals. High percentages of respiratory (19.6%) signs and symptoms, especially among children, were noted, though it was not possible to determine whether individuals infected with CHIKV were co-infected with other pathogens. These results suggest that CHIKV may have been underdiagnosed during this outbreak, and that CHIKF should be included in differential diagnoses of diverse undifferentiated febrile syndromes in the Americas.

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“…In the winter of 2013, autochthonous cases in the Americas were documented (Fischer et al, 2014), with the virus rapidly spreading throughout the Caribbean islands (Lanciotti and Valadere, 2014). The virus has now been disseminated to more than 40 countries in the Americas (Diaz-Quinonez et al, 2015) and likely will pose a continued threat to global human health and economy.…”

Section: Introductionmentioning

confidence: 99%

Neutralizing Monoclonal Antibodies Block Chikungunya Virus Entry and Release by Targeting an Epitope Critical to Viral Pathogenesis

et al. 2015

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Summary We evaluated the mechanism by which neutralizing human monoclonal antibodies inhibit chikungunya virus (CHIKV) infection. Potently neutralizing antibodies (NAbs) blocked infection at multiple steps of the virus life cycle, including entry and release. Cryo-electron microscopy structures of Fab fragments of two human NAbs and chikungunya virus-like particles showed a binding footprint that spanned independent domains on neighboring E2 subunits within one viral spike, suggesting a mechanism for inhibiting low pH-dependent membrane fusion. Detailed epitope mapping identified residue E2-W64 as a critical interaction residue. An escape mutation (E2-W64G) at this residue rendered CHIKV attenuated in mice. Consistent with this data, CHIKV-E2-W64G failed to emerge in vivo under the selection pressure of one of the NAbs, IM-CKV063. As our study suggests that antibodies engaging the residue E2-W64 can potently inhibit CHIKV at multiple stages of infection, antibody-based therapies or immunogens that target this region might have protective value.

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Complete Genome Sequences of Chikungunya Virus Strains Isolated in Mexico: First Detection of Imported and Autochthonous Cases

Cited by 29 publications

References 6 publications

Tomatidine, a natural steroidal alkaloid shows antiviral activity towards chikungunya virus in vitro

Tomatidine, a natural steroidal alkaloid shows antiviral activity towards chikungunya virus in vitro

Molecular Virologic and Clinical Characteristics of a Chikungunya Fever Outbreak in La Romana, Dominican Republic, 2014

Neutralizing Monoclonal Antibodies Block Chikungunya Virus Entry and Release by Targeting an Epitope Critical to Viral Pathogenesis

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