Complete Genome Sequences of Four Extensively Drug-Resistant Pseudomonas aeruginosa Strains, Isolated from Adults with Ventilator-Associated Pneumonia at a Tertiary Referral Hospital in Mexico City

Espinosa-Camacho, Luis F.; Delgado, Gabriela; Soberón‐Chávez, Gloria; Alcaraz, Luis David; Castañón, Jorge; Morales-Espinosa, Rosario

doi:10.1128/genomea.00925-17

Cited by 6 publications

(3 citation statements)

References 12 publications

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“… 19 The ST found in this study has already been reported in different parts of the world 45 and the ST308 in Mexico. 46 So this is the first report that shows the presence of ST167 and ST385 in Mexico.…”

Section: Discussionmentioning

confidence: 78%

Characterization of antimicrobial resistance mechanisms in carbapenem-resistant <em>Pseudomonas aeruginosa</em> carrying IMP variants recovered from a Mexican Hospital

López-García¹,

Rocha-Gracia²,

Bello-López³

et al. 2018

IDR

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PurposePseudomonas aeruginosa infections in hospitals constitute an important problem due to the increasing multidrug resistance (MDR) and carbapenems resistance. The knowledge of resistance mechanisms in Pseudomonas strains is an important issue for an adequate antimicrobial treatment. Therefore, the objective was to investigate other antimicrobial resistance mechanisms in MDR P. aeruginosa strains carrying blaIMP, make a partial plasmids characterization, and determine if modifications in oprD gene affect the expression of the OprD protein.MethodologySusceptibility testing was performed by Kirby Baüer and by Minimum Inhibitory Concentration (presence/absence of efflux pump inhibitor); molecular typing by Pulsed-field gel electrophoresis (PFGE), resistance genotyping and integrons by PCR and sequencing; OprD expression by Western blot; plasmid characterization by MOB Typing Technique, molecular size by PFGE-S1; and blaIMP location by Southern blot.ResultsAmong the 59 studied P. aeruginosa isolates, 41 multidrug resistance and carbapenems resistance isolates were detected and classified in 38 different PFGE patterns. Thirteen strains carried blaIMP; 16 blaGES and four carried both genes. This study centered on the 17 strains har-boring blaIMP. New variants of β-lactamases were identified (blaGES-32, blaIMP-56, blaIMP-62) inside of new arrangements of class 1 integrons. The presence of blaIMP gene was detected in two plasmids in the same strain. The participation of the OprD protein and efflux pumps in the resistance to carbapenems and quinolones is shown. No expression of the porin OprD due to stop codon or IS in the gene was found.ConclusionsThis study shows the participation of different resistance mechanisms, which are reflected in the levels of MIC to carbapenems. This is the first report of the presence of three new variants of β-lactamases inside of new arrangements of class 1 integrons, as well as the presence of two plasmids carrying blaIMP in the same P. aeruginosa strain isolated in a Mexican hospital.

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Section: Discussionmentioning

confidence: 78%

Characterization of antimicrobial resistance mechanisms in carbapenem-resistant <em>Pseudomonas aeruginosa</em> carrying IMP variants recovered from a Mexican Hospital

López-García¹,

Rocha-Gracia²,

Bello-López³

et al. 2018

IDR

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“…Shading denotes regions of homology (nucleotide identity ≥95%). The accession number of Tn 6877 ( Espinosa-Camacho et al, 2017 ) used as reference is CP021775.…”

Section: Resultsmentioning

confidence: 99%

“…Region and bla VEB-3 Region Tn6877 (55.9 kb in length) was used as the prototype IME and initially found in P. aeruginosa Pa58 (Espinosa-Camacho et al, 2017). Tn6877, Tn6878, and a presumed primordial Tn6877related element were all integrated at the same site between the two chromosomal genes orf2892 (a subunit of ribonucleotide reductase) and orf1248 (ß subunit), and they had essentially identical backbones, which was 21.6 kb in length and contained attL/R and two different int genes ( Figure 6).…”

Section: Comparison Of Two Related Imes Tn6877 and Tn6878 And Their Dmentioning

confidence: 99%

Novel Chromosome-Borne Accessory Genetic Elements Carrying Multiple Antibiotic Resistance Genes in Pseudomonas aeruginosa

Yang

et al. 2021

Front. Cell. Infect. Microbiol.

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Pseudomonas aeruginosa is noted for its intrinsic antibiotic resistance and capacity of acquiring additional resistance genes. In this study, the genomes of nine clinical P. aeruginosa isolates were fully sequenced. An extensive genetic comparison was applied to 18 P. aeruginosa accessory genetic elements (AGEs; 13 of them were sequenced in this study and located within P. aeruginosa chromosomes) that were divided into four groups: five related integrative and conjugative elements (ICEs), four related integrative and mobilizable elements (IMEs), five related unit transposons, and two related IMEs and their two derivatives. At least 45 resistance genes, involved in resistance to 10 different categories of antibiotics and heavy metals, were identified from these 18 AGEs. A total of 10 β-lactamase genes were identified from 10 AGEs sequenced herein, and nine of them were captured within class 1 integrons, which were further integrated into ICEs and IMEs with intercellular mobility, and also unit transposons with intracellular mobility. Through this study, we identified for the first time 20 novel MGEs, including four ICEs Tn6584, Tn6585, Tn6586, and Tn6587; three IMEs Tn6853, Tn6854, and Tn6878; five unit transposons Tn6846, Tn6847, Tn6848, Tn6849, and Tn6883; and eight integrons In1795, In1778, In1820, In1784, In1775, In1774, In1789, and In1799. This was also the first report of two resistance gene variants blaCARB-53 and catB3s, and a novel ST3405 isolate of P. aeruginosa. The data presented here denoted that complex transposition and homologous recombination promoted the assembly and integration of AGEs with mosaic structures into P. aeruginosa chromosomes.

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Prediction of Potential Drug Targets and Vaccine Candidates Against Antibiotic-Resistant Pseudomonas aeruginosa

Shamsinejad

Zafari

2022

Int J Pept Res Ther

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Pseudomonas aeruginosa is one of the leading causes of nosocomial infections, characterized by increasing antibiotic resistance, severity and mortality. Therefore, numerous efforts have been made nowadays to identify new therapeutic targets. This study aimed to find potential drug targets and vaccine candidates in drug-resistant strains of P. aeruginosa. Extensive antibiotic-resistant and carbapenem-resistant strains of P. aeruginosa with complete genome were selected and ten common hypothetical proteins (HPs) containing more than 200 amino acids were obtained. The structural, functional and immunological predictions of these HPs were performed with the utility of bioinformatics approaches. Two common HPs (Gene ID: 2877781645 and 2877781936) among other investigated proteins were revealed as potential candidates for pharmaceutical and vaccine purposes based on structural and physicochemical properties, functional domains, subcellular localizations, signal peptides, toxicity, virulence factor, antigenicity, allergenicity and immunoinformatic predictions. The consequence of this predictive study will assist in novel drug and vaccine design through experimental investigations.

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Complete Genome Sequences of Four Extensively Drug-Resistant Pseudomonas aeruginosa Strains, Isolated from Adults with Ventilator-Associated Pneumonia at a Tertiary Referral Hospital in Mexico City

Cited by 6 publications

References 12 publications

Characterization of antimicrobial resistance mechanisms in carbapenem-resistant <em>Pseudomonas aeruginosa</em> carrying IMP variants recovered from a Mexican Hospital

Characterization of antimicrobial resistance mechanisms in carbapenem-resistant <em>Pseudomonas aeruginosa</em> carrying IMP variants recovered from a Mexican Hospital

Novel Chromosome-Borne Accessory Genetic Elements Carrying Multiple Antibiotic Resistance Genes in Pseudomonas aeruginosa

Prediction of Potential Drug Targets and Vaccine Candidates Against Antibiotic-Resistant Pseudomonas aeruginosa

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