Complete Genome Sequences of Four Macrolide-Resistant Nondiphtheritic Corynebacterium Isolates

Abstract

This report describes the complete genome sequences of four isolates of the nondiphtheritic Corynebacterium (NDC) species Corynebacterium pseudodiphtheriticum and Corynebacterium propinquum , recovered during investigation of a large diphtheria outbreak in Bangladesh. These data will assist in better delineating the boundary between these related species and understanding their virulence potential.

Genomic characterization of cocirculating Corynebacterium diphtheriae and non-diphtheritic Corynebacterium species among forcibly displaced Myanmar nationals, 2017–2019

“…Further query of de novo assemblies to investigate the genetic context revealed that ermX appeared in contigs also encoding IS elements. Similarly, the four closed genomes of NDC isolates [ 42 ] indicated that ermX was encoded within the chromosome, rather than plasmids, with sequencing coverage depth comparable to that of the entire genome, where it was also flanked by numerous IS elements. Multiple sequence alignment of the adjacent regions around ermX in these closed genomes further revealed that overall placement within the chromosome was variable (Fig.…”

“…The genetic context of ermX was further analysed in each NDC genome by querying the ermX -containing contig from de novo assembly in ISfinder [ 54 ]. Moreover, 10 kb of flanking sequence upstream and downstream of ermX in four complete NDC genomes [ 42 ] were compared with blast n v2.2.26 and visualized by Easyfig [ 55 ]. In addition, the presence of penicillin-binding proteins (PBP) in C. diphtheriae was further assessed by blast x v2.2.26 [ 52 ] query of assembled contigs for each of the 56 C .…”

“…diphtheriae [32, 37–40], leading to clinical and laboratory diagnostic challenges, particularly during a diphtheria outbreak investigation [8]. Few genomic studies of NDC isolates have been reported and the ecology of these human-associated species, including their potential for causing disease, remains poorly understood [41, 42].…”