Complete Genome Sequences of Four Novel Human Coronavirus OC43 Isolates Associated with Severe Acute Respiratory Infection

Abstract

We report here the complete genome sequences of four human coronavirus (HCoV) OC43 isolates generated using targeted viral nucleic acid capture and next-generation sequencing; the isolates were collected in New Mexico and Arkansas, USA, in February (HCoV-OC43/USA/TCNP_0070/2016) and March (HCoV-OC43/USA/ACRI_0052/2016) 2016 and January 2017 (HCoV-OC43/USA/TCNP_00204/2017 and HCoV-OC43/USA/TCNP_00212/2017).

A complete protocol for whole-genome sequencing of virus from clinical samples: Application to coronavirus OC43

“…Sanger sequencing was the first method used with variable size of amplified regions (St-Jean et al, 2004;Vijgen et al, 2005). Targeted viral nucleic acid capture and RNA sequencing library was recently used to sequence four human HCoV-OC43 isolates (Dinwiddie et al, 2018). The most usual method is to divide the genome into 2.5 kb regions which are amplified, fragmented and sequenced with HTS technologies (Cotten et al, 2013;Taboada et al, 2016).…”