Complete Genome Sequences of Porcine Deltacoronavirus Strains DH1/2016 and DH2/2016 Isolated in South Korea

Chung, Hee‐Chun; Nguyen, Van Giap; Oh, Woo-Taek; Le, Huynh Thi My; Moon, Hyung-Joon; Lee, Jee-Hoon; Kim, Hye-Kwon; Park, Seongjun; Park, Bong Kyun

doi:10.1128/genomea.01706-16

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…Acute cases of PDCoV infection exhibit watery diarrhea in sows and nursing piglets, resulting in severe gastrointestinal disease which may have a lethal outcome [4][5][6]. PDCoV poses a major threat to the swine industry, and is currently epidemic in several countries; first reported in Hong Kong in 2012 [7], it has since been found in the United States [8][9][10], Canada [11], South Korea [12][13][14][15], mainland China [16][17][18][19], Thailand [20][21][22] and Vietnam [23]. Importantly, porcine aminopeptidase N (pAPN) has been reported to serve as a functional receptor for PDCoV in two recent studies [24,25], and the virus may engage APN from diverse species to facilitate its interspecies transmission [25].…”

Section: Introductionmentioning

confidence: 99%

Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner

Yang

Meng

Qin

et al. 2020

Emerging Microbes & Infections

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Porcine deltacoronavirus (PDCoV) is a newly emerging threat to the global porcine industry. PDCoV has been successfully isolated using various medium additives including trypsin, and although we know it is important for viral replication, the mechanism has not been fully elucidated. Here, we systematically investigated the role of trypsin in PDCoV replication including cell entry, cell-to-cell membrane fusion and virus release. Using pseudovirus entry assays, we demonstrated that PDCoV entry is not trypsin dependent. Furthermore, unlike porcine epidemic diarrhea virus (PEDV), in which trypsin is important for the release of virus from infected cells, PDCoV release was not affected by trypsin. We also demonstrated that trypsin promotes PDCoV replication by enhancing cell-to-cell membrane fusion. Most importantly, our study illustrates two distinct spreading patterns from infected cells to uninfected cells during PDCoV transmission, and the role of trypsin in PDCoV replication in cells with different virus spreading types. Overall, these results clarify that trypsin promotes PDCoV replication by mediating cell-to-cell fusion transmission but is not crucial for viral entry. This knowledge can potentially contribute to improvement of virus production efficiency in culture, not only for vaccine preparation but also to develop antiviral treatments.

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Section: Introductionmentioning

confidence: 99%

Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner

Yang

Meng

Qin

et al. 2020

Emerging Microbes & Infections

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“…In addition, a porcine delta CoV (PDCoV) HKU15, was identified in Hong Kong in 2012 (Woo et al, 2012), infecting pig intestinal epithelia and causing severe diarrhea and vomiting (Ma et al, 2015). Several PDCoV strains were later detected in the United States (Alhamo et al, 2022), Canada (Ajayi et al, 2018), South Korea (Chung et al, 2017), Thailand (Janetanakit et al, 2016), China (Huang et al, 2020a), Japan (Suzuki et al, 2018), Vietnam (Le et al, 2018), etc., posing a severe threat to humans population associated with the swine industry. So far, all members of the delta CoV genus have been found in birds (Saeng-Chuto et al, 2017), implying that birds serve as the natural reservoir for these viruses (Jung et al, 2015;Ma et al, 2015).…”

Section: Epidemiology Of Covsmentioning

confidence: 99%

Cross-species transmission, evolution and zoonotic potential of coronaviruses

Shah²,

Wang³

et al. 2023

Front. Cell. Infect. Microbiol.

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Coronaviruses (CoVs) continuously evolve, crossing species barriers and spreading across host ranges. Over the last two decades, several CoVs (HCoV-229E, HCoV-NL63, HCoV-HKU1, HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2) have emerged in animals and mammals, causing significant economic and human life losses. Due to CoV cross-species transmission and the evolution of novel viruses, it is critical to identify their natural reservoiurs and the circumstances under which their transmission occurs. In this review, we use genetic and ecological data to disentangle the evolution of various CoVs in wildlife, humans, and domestic mammals. We thoroughly investigate several host species and outline the epidemiology of CoVs toward specific hosts. We also discuss the cross-species transmission of CoVs at the interface of wildlife, animals, and humans. Clarifying the epidemiology and diversity of species reservoirs will significantly impact our ability to respond to the future emergence of CoVs in humans and domestic animals.

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“…In early 2014, an outbreak of PDCoV-associated diarrhea broke out in pig farms in Ohio, United States, which led to the identification of the first clinical case of PDCoV infection in pigs [ 2 , 4 , 7 , 8 ]. Since then, PDCoV has been detected worldwide, including in Korea, Japan, China, Thailand, Vietnam, Laos, Peru, and Mexico [ 3 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. According to previous phylogenetic analyses, PDCoV can be divided into three or four groups based on their full-length genome sequences [ 15 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…KM820765) was first identified in April 2014 from the fecal samples of pigs with diarrhea [ 17 ]. Subsequent studies have shown that all Korean PDCoV strains identified during 2014–2016 have a high sequence identity with strains reported in the United States [ 10 , 18 , 21 ]. However, since 2016, there have been no reports of PDCoV outbreaks in Korea.…”

Section: Introductionmentioning

confidence: 99%

Genetic Characterization and Evolution of Porcine Deltacoronavirus Isolated in the Republic of Korea in 2022

et al. 2023

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Porcine deltacoronavirus (PDCoV) is an emerging coronavirus that causes diarrhea in nursing piglets. Since its first outbreak in the United States in 2014, this novel porcine coronavirus has been detected worldwide, including in Korea. However, no PDCoV case has been reported since the last report in 2016 in Korea. In June 2022, the Korean PDCoV strain KPDCoV-2201 was detected on a farm where sows and piglets had black tarry and watery diarrhea, respectively. We isolated the KPDCoV-2201 strain from the intestinal samples of piglets and sequenced the viral genome. Genetically, the full-length genome and spike gene of KPDCoV-2201 shared 96.9–99.2% and 95.8–98.8% nucleotide identity with other global PDCoV strains, respectively. Phylogenetic analysis suggested that KPDCoV-2201 belongs to G1b. Notably, the molecular evolutionary analysis indicated that KPDCoV-2201 evolved from a clade different from that of previously reported Korean PDCoV strains and is closely related to the emergent Peruvian and Taiwanese PDCoV strains. Furthermore, KPDCoV-2201 had one unique and two Taiwanese strain-like amino acid substitutions in the receptor-binding domain of the S1 region. Our findings suggest the possibility of transboundary transmission of the virus and expand our knowledge about the genetic diversity and evolution of PDCoV in Korea.

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Complete Genome Sequences of Porcine Deltacoronavirus Strains DH1/2016 and DH2/2016 Isolated in South Korea

Cited by 5 publications

References 9 publications

Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner

Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner

Cross-species transmission, evolution and zoonotic potential of coronaviruses

Genetic Characterization and Evolution of Porcine Deltacoronavirus Isolated in the Republic of Korea in 2022

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