Complete Genome Sequences of Thermus thermophilus Strains HB5002 and HB5008, Isolated from Mine Hot Spring in Japan

Abstract

We isolated Thermus thermophilus strains HB5002 and HB5008 from Mine Hot Spring in Japan. Whole-genome sequencing revealed that they showed ∼100% average nucleotide identity to each other, ≥98.53% to the T. thermophilus strains originating from the same spot but ≤97.64% to the T. thermophilus strains from geographically different places in Japan.

Complete Genome Sequences of Four Halophilic Thermus thermophilus Strains Isolated from Arima Hot Spring in Japan

“…Automatic annotation was conducted using DFAST v.1.4.0 ( 13 ); the genomic features are summarized in Table 1 . A JSpecies analysis ( 14 ) revealed that the genome sequences of bacterial strains from Arima Hot Spring (four strains presented in this study and two previously documented strains, AA2-20 [ 15 ] and AA2-29 [ 15 ]) showed ANIs of ≥99.21% to each other but of ≤97.86% to six strains originating from Mine Hot Spring (HB8, HB27 [ 4 ], HC11 [ 5 ], HB5002 [ 7 ], HB5008 [ 7 ], and HB5018 [ 6 ]).…”

“…So far, complete genome sequences have been reported for 13 strains ( https://www.ncbi.nlm.nih.gov/genome/browse#!/prokaryotes/461/ ), of which six were isolated from Mine Hot Spring. Comparative genomic analyses ( 3 ) of these six strains ( 4 – 7 ) revealed high (≥98.53%) average nucleotide identities (ANIs) to each other, supporting habitat-specific genomic conservation. In this study, to gain insight into the habitat-dependent evolutionary patterns of T. thermophilus , we investigated the genomes of four T. thermophilus strains that were newly isolated from Arima Hot Spring in Japan (34.7974 N, 135.2494 E).…”

Complete Genome Sequences of Thermus Strains Isolated from Senami Hot Spring in Japan

“…Due to its high-temperature adaptation at around 70°C, Thermus has great biotechnological potential as a source of thermophilic enzymes, as exemplified by Taq DNA polymerase ( 4 ). Since the discovery of T. aquaticus , many Thermus strains have been isolated from thermal areas worldwide ( 5 – 11 ).…”