Complete Genome Sequences of Three Border Disease Virus Strains of the Same Subgenotype, BDSwiss, Isolated from Sheep, Cattle, and Pigs in Switzerland

Stalder, Hanspeter; Marti, Sabrina; Flückiger, Franziska; Renevey, Nathalie; Hofmann, Martin; Schweizer, Matthias

doi:10.1128/genomea.01238-17

Cited by 9 publications

(9 citation statements)

References 13 publications

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“…All cows underwent oestrus synchronisation and were artificially inseminated twice 24 h apart (day 0 and day 1 of the infection phase) using semen from a young bull persistently infected with BDV [ 10 , 11 ]. The genome of the virus causing persistent infection (Sub-Genotype BDSwiss) has since been sequenced [ 16 ]. The semen had a high virus titre at 2.51 × 10 8 TCID 50 (50% tissue culture infective dose)/ml and 1.44 × 10 6 TCID 50 /10 6 sperm cells.…”

Section: Methodsmentioning

confidence: 99%

Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses

et al. 2018

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BackgroundThis study examined various health variables in cows after artificial insemination with Border disease virus (BDV)-infected semen and the occurrence of persistent infection in ensuing fetuses. Five cows were inseminated (day 0) with BDV-infected semen as well as with semen from a fertile Eringer bull. One cow, inseminated with virus-free semen only, served as a control. Clinical examination, assessment of eating and rumination activities, measurement of intraruminal temperature and leukocyte count were used to monitor the health of the cows. Blood samples were collected at regular intervals for the detection of viral RNA and antibodies against BDV, and the cows were slaughtered on day 56. The uteri, placentae and fetuses were examined macroscopically, histologically, immunohistochemically and by means of molecular methods for the presence of pestiviruses.ResultsThe demeanour, eating and rumination activities and intraruminal temperature were not affected by insemination with BDV-infected semen, whereas the total leukocyte and lymphocyte counts dropped transiently and were significantly lower on day 6 than on day 0. Seroconversion occurred by day 28 in the five infected cows but not in the control cow. The uteri, placentae and fetuses had no macroscopic or histological lesions, and immunohistochemical examination and RT-PCR were negative for pestiviruses.ConclusionsThe findings showed that cows inseminated with BDV-infected semen seroconverted and fetuses thus produced were not persistently infected. Transmission of BDV to cattle through infected semen, therefore, seems to be of minor importance.

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Section: Methodsmentioning

confidence: 99%

Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses

et al. 2018

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“…As the 5′-UTR is relatively more conserved, it is used to define the pan-pestivirus reactive primer regions and is frequently used in genotyping studies [14]. Currently, there are at least 8 BDV genotypes from BDV-1 to BDV-8 [10, 15].…”

Section: Introductionmentioning

confidence: 99%

First report of border disease virus in Melophagus ovinus (sheep ked) collected in Xinjiang, China

Liu¹,

He²,

Li³

et al. 2019

PLoS ONE

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Melophagus ovinus (sheep ked) is a blood-sucking ectoparasite that is parasitic primarily on sheep. It is widely distributed in different geographical regions worldwide. In China, it has been mainly found in Xinjiang, Gansu, and Tibet in recent years. In addition to causing direct damage to the animal hosts, M . ovinus also carries pathogens and serves as a vector for disease transmission. Border disease virus (BDV) is a positive-sense, single-stranded RNA pestivirus that mainly infects and causes border disease (BD) in sheep and goats worldwide. Since 2012, this disease has been reported in 4 provinces in China. In the present study, we investigated the presence of BDV in M . ovinus from Xinjiang and Gansu. Frozen M . ovinus collected during 2017 and 2018 from Xinjiang and Gansu and preserved in our laboratory were studied. First, total RNA of M . ovinus was extracted, followed by reverse transcription, PCR (RT-PCR) amplification of the 5′-UTR of BDV, and sequencing of the amplified products. Finally, the sequencing results were analyzed using DNAStar, MEGA 5.0 molecular biology software, and the BLAST online platform. The results from RT-PCR and sequencing analyses showed that among the samples included in the study, only the M . ovinus collected from Qinghe County in Alta, Xinjiang in 2018 tested positive for BDV. BLAST analysis showed that the viral strain with the most similar nucleotide identity to the sequence of the China/BDV/2018 fragment was the goat-derived BDV strain AH12-02 collected in Anhui, China, in 2012. A phylogenetic-tree analysis showed the strain to exhibit a BDV-3 genotype. This is the first report globally on BDV detected in M . ovinus and is also the first report of BDV discovered in Xinjiang, China. This study reconfirms the presence of BDV in China.

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“…Q739K) could not compensate for the sheep K771E substitution in pig cells, suggesting that the presence of K739 is important for pig adaptation. Indeed, another pig isolate of BDV has the same change [34]. We did observe that the suppression of propagation by the K771E substitution could be rescued by another change in E2 (Q728R) that occurred in the pig cells.…”

Section: Discussionmentioning

confidence: 71%

Identification of specific amino acid residues in the border disease virus glycoprotein E2 that modify virus growth in pig cells but not in sheep cells

Fahnøe

Deng

Davids

et al. 2020

Journal of General Virology

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Border disease virus (BDV) envelope glycoprotein E2 is required for entry into cells and is a determinant of host tropism for sheep and pig cells. Here, we describe adaptive changes in the BDV E2 protein that modify virus replication in pig cells. To achieve this, two BDV isolates, initially collected from a pig and a sheep on the same farm, were passaged in primary sheep and pig cells in parallel with a rescued variant of the pig virus derived from a cloned full-length BDV cDNA. The pig isolate and the rescued virus shared the same amino acid sequence, but the sheep isolate differed at ten residues, including two substitutions in E2 (K771E and Y925H). During serial passage in cells, the viruses displayed clear selectivity for growth in sheep cells; only the cDNA-derived virus adapted to grow in pig cells. Sequencing revealed an amino acid substitution (Q739R) in the E2 domain DA of this rescued virus. Adaptation at the same residue (Q739K/Q739R) was also observed after passaging of the pig isolate in sheep cells. Use of reverse genetics confirmed that changing residue Q739 to R or K (each positively charged) was sufficient to achieve adaptation to pig cells. Furthermore, this change in host tropism was suppressed if Q739R was combined with K771E. Another substitution (Q728R), conferring an additional positive charge, acquired during passaging, restored the growth of the Q739R/K771E variant. Overall, this study provided evidence that specific, positively charged, residues in the E2 domain DA are crucial for pig-cell tropism of BDV.

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Complete Genome Sequences of Three Border Disease Virus Strains of the Same Subgenotype, BDSwiss, Isolated from Sheep, Cattle, and Pigs in Switzerland

Cited by 9 publications

References 13 publications

Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses

Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses

First report of border disease virus in Melophagus ovinus (sheep ked) collected in Xinjiang, China

Identification of specific amino acid residues in the border disease virus glycoprotein E2 that modify virus growth in pig cells but not in sheep cells

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