Complete Genome Sequencing of Protease-Producing Novel
            <i>Arthrobacter</i>
            sp. Strain IHBB 11108 Using PacBio Single-Molecule Real-Time Sequencing Technology

Kiran, Shashi; Swarnkar, Mohit Kumar; Pal, Mohinder; Thakur, Rishu; Tewari, Rupinder; Singh, Anil Kumar; Gulati, Arvind

doi:10.1128/genomea.00346-15

Cited by 14 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some alkaliphilic actinobacterial species belonging to the genus Streptomyces (Mikami et al, 1982 ), Micromonospora (Jiang and Xu, 1993 ), Nocardioides (Yoon et al, 2005 ), Microcella (Tiago et al, 2005 ), Cellulomonas (Jones et al, 2005 ), Nesterenkonia (Luo et al, 2009 ), Streptosporangium (Gurielidze et al, 2010 ), Corynebacterium (Wu et al, 2011b ), Georgenia (Srinivas et al, 2012 ), Nocardiopsis, Isoptericola, Nesterenkonia (Ara et al, 2013 ), Saccharomonospora (Raut et al, 2013 ), Saccharothrix (Jani et al, 2014 ), and Arthrobacter (Kiran et al, 2015 ) have been isolated and well-characterized. Among them, the genus Nocardiopsis has been found to be prominent in alkaline environments (Ara et al, 2013 ).…”

Section: Alkaliphilic and Alkalitolerant Actinobacteriamentioning

confidence: 99%

Thermophilic and alkaliphilic Actinobacteria: biology and potential applications

2015

View full text Add to dashboard Cite

Microbes belonging to the phylum Actinobacteria are prolific sources of antibiotics, clinically useful bioactive compounds and industrially important enzymes. The focus of the current review is on the diversity and potential applications of thermophilic and alkaliphilic actinobacteria, which are highly diverse in their taxonomy and morphology with a variety of adaptations for surviving and thriving in hostile environments. The specific metabolic pathways in these actinobacteria are activated for elaborating pharmaceutically, agriculturally, and biotechnologically relevant biomolecules/bioactive compounds, which find multifarious applications.

show abstract

Section: Alkaliphilic and Alkalitolerant Actinobacteriamentioning

confidence: 99%

Thermophilic and alkaliphilic Actinobacteria: biology and potential applications

2015

View full text Add to dashboard Cite

show abstract

“…In comparison, the smallest Arthrobacter plasmid known until now, pAG001 (3.7 kb) of Arthrobacter sp. IHBB 11108, contains 6 genes (Kiran et al, 2015), while the largest, unnamed2 (754.2 kb), isolated from strain ERGS1:01, carries 675 genes (Kumar et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Insight Into the Diversity and Possible Role of Plasmids in the Adaptation of Psychrotolerant and Metalotolerant Arthrobacter spp. to Extreme Antarctic Environments

2018

View full text Add to dashboard Cite

Arthrobacter spp. are coryneform Gram-positive aerobic bacteria, belonging to the class Actinobacteria. Representatives of this genus have mainly been isolated from soil, mud, sludge or sewage, and are usually mesophiles. In recent years, the presence of Arthrobacter spp. was also confirmed in various extreme, including permanently cold, environments. In this study, 36 psychrotolerant and metalotolerant Arthrobacter strains isolated from petroleum-contaminated soil from the King George Island (Antarctica), were screened for the presence of plasmids. The identified replicons were thoroughly characterized in order to assess their diversity and role in the adaptation of Arthrobacter spp. to harsh Antarctic conditions. The screening process identified 11 different plasmids, ranging in size from 8.4 to 90.6 kb. A thorough genomic analysis of these replicons detected the presence of numerous genes encoding proteins that potentially perform roles in adaptive processes such as (i) protection against ultraviolet (UV) radiation, (ii) resistance to heavy metals, (iii) transport and metabolism of organic compounds, (iv) sulfur metabolism, and (v) protection against exogenous DNA. Moreover, 10 of the plasmids carry genetic modules enabling conjugal transfer, which may facilitate their spread among bacteria in Antarctic soil. In addition, transposable elements were identified within the analyzed plasmids. Some of these elements carry passenger genes, which suggests that these replicons may be actively changing, and novel genetic modules of adaptive value could be acquired by transposition events. A comparative genomic analysis of plasmids identified in this study and other available Arthrobacter plasmids was performed. This showed only limited similarities between plasmids of Antarctic Arthrobacter strains and replicons of other, mostly mesophilic, isolates. This indicates that the plasmids identified in this study are novel and unique replicons. In addition, a thorough meta-analysis of 247 plasmids of psychrotolerant bacteria was performed, revealing the important role of these replicons in the adaptation of their hosts to extreme environments.

show abstract

“…The quality and quantity of extracted genomic DNA were determined by a Qubit 2.0 fluorometer (Invitrogen, USA). Genomic DNA was sheared using Covaris g-Tubes to construct a library with a 10-kb DNA insert size using the PacBio SMRTbell template preparation kit version 1.0, as described earlier ( 8 ). The prepared SMRTbell library was sequenced using the PacBio RS II system on two SMRT cells using P6 polymerase-C4 sequencing chemistry with a 240-min movie.…”

Section: Genome Announcementmentioning

confidence: 99%

Genome Assembly of Chryseobacterium polytrichastri ERMR1:04, a Psychrotolerant Bacterium with Cold Active Proteases, Isolated from East Rathong Glacier in India

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Complete Genome Sequencing of Protease-Producing Novel Arthrobacter sp. Strain IHBB 11108 Using PacBio Single-Molecule Real-Time Sequencing Technology

Cited by 14 publications

References 12 publications

Thermophilic and alkaliphilic Actinobacteria: biology and potential applications

Thermophilic and alkaliphilic Actinobacteria: biology and potential applications

Insight Into the Diversity and Possible Role of Plasmids in the Adaptation of Psychrotolerant and Metalotolerant Arthrobacter spp. to Extreme Antarctic Environments

Genome Assembly of Chryseobacterium polytrichastri ERMR1:04, a Psychrotolerant Bacterium with Cold Active Proteases, Isolated from East Rathong Glacier in India

Contact Info

Product

Resources

About