Complete genomic characterization of a Chinese isolate of porcine reproductive and respiratory syndrome virus

Zhu, Ling; Zhang, Guanqun; Ma, Jingyun; He, Xiaoming; Xie, Qingmei; Bee, Yingzuo; Gong, Shan Zhong

doi:10.1016/j.vetmic.2010.07.001

Cited by 24 publications

(15 citation statements)

References 31 publications

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“…However, it is possible that these isolates were never widespread because the large deletions may affect the ability of the virus to survive (12). In addition to the deletions mentioned above, other types of deletions, i.e., of 12 amino acids, 68 amino acids, 65 amino acids, 51 amino acids, and 131 amino acids, have been observed in other isolates in previous studies (14,16,19,29,45). It is interesting that the deletions in the viral genomes of field PRRSV isolates occur mostly in the Nsp2 region.…”

Section: Discussionmentioning

confidence: 92%

Epidemiology and Evolutionary Characteristics of the Porcine Reproductive and Respiratory Syndrome Virus in China between 2006 and 2010

Fang

Guo

et al. 2011

J Clin Microbiol

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In 2006, an emerging highly pathogenic strain of porcine reproductive and respiratory syndrome virus (PRRSV), which causes continuous high fever and a high proportion of deaths in vaccinated pigs of all ages, broke out in mainland China and spread rapidly to neighboring countries. To examine the epidemiology and evolutionary characteristics of Chinese PRRSV after the 2006 outbreak, we tested 2,981 clinical samples collected from 2006 to 2010 in China, determined 153 Nsp2 sequences and 249 ORF5 sequences, and analyzed the epidemiology and genetic diversity of Chinese PRRSV. Our results showed that the percentage of PRRSV-positive specimens collected from sick pigs averaged 60.85% in the past 5 years and that the highly pathogenic PRRSV has become the dominant strain in China. Furthermore, a reemerging strain which apparently evolved from the highly pathogenic PRRSV strain in 2006 appeared to be widely prevalent in China from 2009 onwards. Sequence analyses revealed that the hypervariable region of Nsp2 in most of the isolates contained a discontinuous deletion equivalent to 30 amino acids, along with other types of deletions. Extensive amino acid substitutions in the GP5 sequence translated from ORF5 were found, particularly in the potential neutralization epitope and the N-glycosylation sites. Our results suggest that Chinese PRRSV has undergone rapid evolution and can circumvent immune responses induced by currently used vaccines. Information from this study will help in understanding the evolutionary characteristics of Chinese PRRSV and assist ongoing efforts to develop and use PRRSV vaccines in the future.

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Section: Discussionmentioning

confidence: 92%

Epidemiology and Evolutionary Characteristics of the Porcine Reproductive and Respiratory Syndrome Virus in China between 2006 and 2010

Fang

Guo

et al. 2011

J Clin Microbiol

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“…A porcine reproductive and respiratory syndrome virus (PRRSV) field isolate, named GM2, was propagated on Marc-145 cells, and total viral RNA was extracted from the serum and infected cell culture and then used separately for the amplification of the genome. Basically, 16 primer pairs were used to generate overlapping amplicons by reverse transcription-PCR (RT-PCR) as reported previously (8), and 3=-terminal sequences of GM2 were obtained by a 3= rapid amplification of cDNA ends (RACE kit; TaKaRa). The PCR products were cloned into pMD19-T vector (TaKaRa), sequenced three times using an ABI 3730 Sanger-based genetic analyzer, and assembled using DNAStar version 7.0 to obtain the complete genome sequence.…”

mentioning

confidence: 99%

Complete Genome Sequence of a Novel Variant Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Strain: Evidence for Recombination between Vaccine and Wild-Type PRRSV Strains

Wei

Zhang

et al. 2012

J Virol

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Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiologic agent of porcine reproductive and respiratory syndrome (PRRS), which can evolve continuously by random mutation or intragenic recombination. Here we report the complete genomic sequence of a PRRSV variant with nucleotide acid deletions and insertions in the nonstructural protein 2 (nsp2) gene and a possible recombination event between a modified live virus (MLV) vaccine strain and a prototype Chinese field strain. P orcine reproductive and respiratory syndrome (PRRS) outbreaks occur worldwide and cause significant economic losses in the swine industry (6, 7). Even though modified live virus (MLV) vaccines were shown to provide solid protection against PRRSV infection, there were substantial barriers to the use of MLV vaccines in the field (2, 5). Considering the extensive use of MLV vaccines for preventing and controlling PRRS in mainland China, the appearance of a natural recombination event between a vaccine strain and a field strain is noteworthy.Serum and tissue samples were collected from suspected growing pigs in Guangdong Province in 2011. Infected pigs were about 10 weeks old and displayed symptoms similar to those of original PRRS, including severe respiratory problems, diarrhea, and poor growth. In addition, this disease resulted in a ϳ2.2% mortality rate on this farm. A porcine reproductive and respiratory syndrome virus (PRRSV) field isolate, named GM2, was propagated on Marc-145 cells, and total viral RNA was extracted from the serum and infected cell culture and then used separately for the amplification of the genome. Basically, 16 primer pairs were used to generate overlapping amplicons by reverse transcription-PCR (RT-PCR) as reported previously (8), and 3=-terminal sequences of GM2 were obtained by a 3= rapid amplification of cDNA ends (RACE kit; TaKaRa). The PCR products were cloned into pMD19-T vector (TaKaRa), sequenced three times using an ABI 3730 Sanger-based genetic analyzer, and assembled using DNAStar version 7.0 to obtain the complete genome sequence. The completed sequence showed that, excluding the poly(A) tail, the genomic sequence of GM2 was 15,513 nucleotides in length. Genetic analysis demonstrated that GM2 shared 90.2% genome similarity with VR-2332, but a lower degree of genetic homology (87.7%) was observed than with the representative highly pathogenic PRRSV JXA1 strain. Compared with the VR-2332 strain, 36-amino-acid insertions and two continuous amino acid deletions in the nonstructural protein 2 (nsp2) region of GM2 were found. However, GM2 shared a very low identity with the SP strain (68.8%) and the Japanese strain EDRD-1 (70.5%), which was characterized by similar insertions within nsp2.Recombination events were detected using both Simplot version 3.5.1 (3) and the recombination detection program version 4.1.3 (RDP4) (4). The results indicated that GM2 was a potential recombinant between the MLV RespPRRS/Repro vaccine strain and a recently emerging prototype Chinese field strain, named QYYZ. P...

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“…Amplicon with molecular size of 490 bp containing complete ORF7 (372 bp) was amplified using ORF7 specific primer pairs [15] . For DNA sequencing, PCR products were purified using a QIAquick Extraction Kit (Qiagen, Germany) and directly sequenced in both directions (Macrogen, Korea).…”

Section: Methodsmentioning

confidence: 99%

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Thúy¹,

Thu²,

Son³

et al. 2015

Kafkas Univ Vet Fak Derg

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Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases for the swine industry worldwide. The 372-bp complete nuclear capsid protein (N-protein) encoding gene (ORF7) of 36 field PRRSV isolates from Vietnam collected during 2008-2012 were sequenced and compared with certain vaccines and published strains of PRRSV. The ORF7 nucleotide sequence (nt) similarity and amino acid (aa) identity among 36 strains showed the highest and ranged from 95.1 to 100%. These isolates shared similarities with VR-2332 (nt 91.9-94.3%, aa 92.6-96.7%) and LV (nt 62.7-65.3%, aa 58.5-60.1%). There were higher level of similarity with QN07 (nt 96.2-99.1%, aa 96.7-99.1%) from the 2007 PRRS outbreak in Quang Nam province, CH-1a (nt 93.0-96.2%, aa 92.6-95.9%) isolated in China in 1995 and JXA1 (nt 96.7-99.7%, aa 97.5-100%), the highly pathogenic strain from China isolated in 2006. Six aa mutations located in both variable and conserved regions of N-protein amino acid sequence were detected in most of the 36 isolates and highly pathogenic PRRSV strains in China in comparison to the prototype strain VR-2332. Results of sequence analyses indicated that PRRSVs isolated in Vietnam during 2008-2012 were classified as North American genotype. Phylogenetic tree also clustered those 36 PRRSV isolates, other recently reported Vietnamese strains, highly pathogenic Chinese strains and JXA1-R vaccine strain in the same cluster and separated from the prototype strain (VR-2332) and Ingelvac MLV/Besta vaccine strains. The result on genetic characterization of ORF7 of circulating PRRSV strains may assist the development of effective strategies for monitoring and controlling PRRS in Vietnam. Keywords: Genetic variation of ORF7, North American genotype, Phylogenetic analysis, PRRSV Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni'nin Genetik Analizi

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Complete genomic characterization of a Chinese isolate of porcine reproductive and respiratory syndrome virus

Cited by 24 publications

References 31 publications

Epidemiology and Evolutionary Characteristics of the Porcine Reproductive and Respiratory Syndrome Virus in China between 2006 and 2010

Epidemiology and Evolutionary Characteristics of the Porcine Reproductive and Respiratory Syndrome Virus in China between 2006 and 2010

Complete Genome Sequence of a Novel Variant Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Strain: Evidence for Recombination between Vaccine and Wild-Type PRRSV Strains

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

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