Complete Genomic Sequence of the Filamentous Nitrogen-fixing Cyanobacterium Anabaena sp. Strain PCC 7120

Kaneko, Takakazu; Nakamura, Yasukazu; Wölk, C. Peter; Kuritz, Tanya; Sasamoto, Shigemi; Watanabe, Akïharu; Iriguchi, Mayumi; Ishikawa, Atsuko; Kawashima, Kumiko; Kimura, Takaharu; Kishida, Yoshie; Kohara, Mitsuyo; Matsumoto, Midori; Matsuno, Ai; Muraki, Akiko; Nakazaki, Naomi; Shimpo, Sayaka; Sugimoto, Masako; Takazawa, Masaki; Yamada, Manabu; Yasuda, Miho; Tabata, Satoshi

doi:10.1093/dnares/8.5.205

Cited by 629 publications

(449 citation statements)

References 38 publications

Supporting

Mentioning

444

Contrasting

Unclassified

Order By: Relevance

“…PCC 7120 (19), an ORF all1173 of 188 codons was revealed. The putative protein (annotated as 'DNA binding protein, starvation inducible') shares high identity with the amino acid sequence of Dps from E. coli (36%), A. tumefaciens (41%), and L. innocua (29%) respectively (Fig.…”

Section: Sequence Analysis and Transcriptional Identification Of Orfmentioning

confidence: 99%

Identification and biochemical properties of Dps (starvation‐induced DNA binding protein) from cyanobacterium Anabaena sp. PCC 7120

Xiong

et al. 2007

IUBMB Life

View full text Add to dashboard Cite

DNA‐binding Proteins from Starved cells (Dps) are anti‐stress iron proteins preserving bacteria from oxidative damage. Based on sequence alignment, a 564‐bp open reading frame (all1173) encoding product in Anabaena sp. PCC 7120 shared high similarity with Dps family proteins. RT‐PCR showed all1173 is active at transcriptional level in Anabaena sp. PCC 7120 cells. We accordingly cloned the all1173 into prokaryotic expression system, purified the corresponding recombinant protein (Dps1173) and characterized its properties in vitro. According to CD spectrum and non‐denaturing electrophoresis assays, recombinant Dps1173 was alpha helix riched, and was likely to form dodecametric oligomer under native conditions. Fluorescence titration experiment revealed two major iron binding sites within Dps1173 monomer, indicating its potential ferroxidase activity. Although phenomena of direct DNA binding was not observed in Electrophoretic mobility shift assay, Dps1173 could also protect DNA from H2O2 stress for its iron scavenging capacity. This is the first description of Dps from heterocystous cyanobacterium Anabaena sp. PCC 7120. IUBMB Life, 59: 675‐681, 2007

show abstract

Section: Sequence Analysis and Transcriptional Identification Of Orfmentioning

confidence: 99%

Identification and biochemical properties of Dps (starvation‐induced DNA binding protein) from cyanobacterium Anabaena sp. PCC 7120

Xiong

et al. 2007

IUBMB Life

View full text Add to dashboard Cite

show abstract

“…Anabaena sp. PCC 7120 has been chosen as a model strain to improve nitrogenasebased H 2 production (Masukawa et al 2002a) because it is amenable to genetic engineering (Elhai and Wolk 1988) and its complete genomic sequence is available (Kaneko et al 2001). As this organism contains both types of hydrogenases, each individual and both hydrogenases were inactivated by targeted gene disruption, producing two single mutants, DHup and DHox, and a double mutant, DHupDHox (Masukawa et al 2002a).…”

Section: Inactivation Of Uptake Hydrogenase (Hup)mentioning

confidence: 99%

“…The cyanobacterium Anabaena sp. PCC 7120 has two homocitrate synthase genes, nifV1 and nifV2, in its chromosome (Kaneko et al 2001). With the DHup strain as the parental strain, two single gene disruption mutants, DHupDNifV1 and DHupDNifV2, and a double gene disruption mutant, DHupDNifV1DNifV2, were constructed .…”

Section: Modification Of the Catalytic Active Center Of Nitrogenasementioning

confidence: 99%

Genetic Engineering of Cyanobacteria to Enhance Biohydrogen Production from Sunlight and Water

Masukawa

Kitashima

Inoue

et al. 2012

AMBIO

View full text Add to dashboard Cite

To mitigate global warming caused by burning fossil fuels, a renewable energy source available in large quantity is urgently required. We are proposing large-scale photobiological H 2 production by mariculture-raised cyanobacteria where the microbes capture part of the huge amount of solar energy received on earth's surface and use water as the source of electrons to reduce protons. The H 2 production system is based on photosynthetic and nitrogenase activities of cyanobacteria, using uptake hydrogenase mutants that can accumulate H 2 for extended periods even in the presence of evolved O 2 . This review summarizes our efforts to improve the rate of photobiological H 2 production through genetic engineering. The challenges yet to be overcome to further increase the conversion efficiency of solar energy to H 2 also are discussed.

show abstract

“…This problem has attracted the attention of researchers worldwide which has led to the evolution of more reliable methods for assessing cyanobacterial taxonomy and diversity like DNA base composition studies (Kaneko et al 2001), DNA hybridization-based assessments (Kondo et al 2000), gene sequencing approaches (Nübel et al 1997), and PCR fingerprinting strategies (Versalovic et al 1991;Rasmussen and Svenning 1998;Shalini and Gupta 2008) PCR-based techniques based especially on DNA polymorphism and fingerprinting of repetitive DNA fragments have been developed and applied in cyanobacterial phylogenetic studies. RFLP (Iteman et al 2002), RAPD (Prabina et al 2005), STRR (Wilson et al 2000;Chonudomkul et al 2004;Valério et al 2009;Akoijam and Singh 2011), and highly iterated palindromes (HIP1) (Orcutt et al 2002;Zheng et al 2002;Neilan et al 2003;Wilson et al 2005) have been attempted with an overall aim to provide better resolution amongst closely related species.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of heterocystous cyanobacteria (Subsections IV and V) using highly iterated palindromes as molecular markers

Singh

Kaushik

Srivastava

et al. 2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Highly iterated palindromes (HIP) have been used as high resolution molecular markers for assessing the genetic variability and phylogenetic relatedness of heterocystous cyanobacteria (subsections IV and V) representing 12 genera of heterocystous cyanobacteria, collected from different geographical areas of India. DNA fingerprints generated using four HIP markers viz. HIP-AT, HIP-CA, HIP-GC, and HIP-TG showed 100 % polymorphism in all the heterocystous cyanobacteria studied and each marker produced unique and strain-specific banding pattern. Furthermore, phylogenetic affinities based on the dendrogram constructed using HIP DNA profiles of heterocystous cyanobacteria suggest the monophyletic origin of this entire heterocystous clade along with a clear illustration of the polyphyletic origin of the branched Stigonematalean order (Subsection V). In addition, phylogenetic affinities were validated by principal component analysis of the HIP fingerprints. The overall data obtained by both the phylogeny and principal component assessments proved that the entire heterocystous clade was intermixed, and there are immediate needs for classificatory reforms that satisfy morphological plasticity and environmental concerns.

show abstract

Complete Genomic Sequence of the Filamentous Nitrogen-fixing Cyanobacterium Anabaena sp. Strain PCC 7120

Cited by 629 publications

References 38 publications

Identification and biochemical properties of Dps (starvation‐induced DNA binding protein) from cyanobacterium Anabaena sp. PCC 7120

Identification and biochemical properties of Dps (starvation‐induced DNA binding protein) from cyanobacterium Anabaena sp. PCC 7120

Genetic Engineering of Cyanobacteria to Enhance Biohydrogen Production from Sunlight and Water

Phylogenetic analysis of heterocystous cyanobacteria (Subsections IV and V) using highly iterated palindromes as molecular markers

Contact Info

Product

Resources

About