Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China

Abstract

A strain of transmissible gastroenteritis virus (TGEV), SHXB, was isolated in Shanghai, China. The complete genome of strain SHXB was sequenced, and its sequence was compared those of other TGEV strains in the GenBank database. The comparison showed that there were no insertions or deletions in the 5' and 3'- non-translated regions, in the nonstructural genes ORF1, ORF3, and ORF7, or in the genes encoding the structural proteins envelope (E), membrane (M) and nucleoprotein (N). A phenomenon in common with other strains was that nucleotide (nt) 655 of the spike (S) gene was G, and a common change in nt 1753 of the S gene was a T-to-G mutation that caused a serine-to-alanine mutation at amino acid 585, which is in the region of the main major antigenic sites A and B of the TGEV S protein. A 6-nt deletion was also found at nt 1123-1128 in all Purdue strains except the strain Virulent Purdue. Phylogenetic analysis showed that TGEV SHXB was closely related to the Purdue strains and shared a common ancestor with the Miller strains as well as strain PRCV-ISU-1.

ORF3adeletion in field strains of porcine-transmissible gastroenteritis virus in China: A hint of association with porcine respiratory coronavirus

“…CH‐SY and CH‐ZB were closely correlated with Miller M6, which is a virulent TGEV strain, while CH‐SH was correlated with Miller M60, which is an attenuated TGEV strain. Previous reports showed that the Purdue cluster also occurred in China (Weiwei et al., ). The current results thus indicate that the Miller, as well as the Purdue cluster, exists in Chinese field strains of TGEV.…”

“…Transmissible gastroenteritis virus is currently divided into two distinct genogroups: the Miller cluster and the Purdue cluster (Hu et al., ). Transmissible gastroenteritis virus occurs in suckling piglets in swine‐producing areas in China (Weiwei et al., ). The TGEV genome was first sequenced by the analysis of polymerase chain reaction (PCR) products in 1993 (Chen, Pocock, & Britton, ).…”

Epidermal growth factor receptor is a co-factor for transmissible gastroenteritis virus entry

“…Transmissible gastroenteritis virus (strain SHXB) was isolated in Shanghai, China. The complete genome sequence for TGEV SHXB is available in GenBank (KP202848.1) (Weiwei et al, 2014). To analyze viral entry, cells were incubated with TGEV at a multiplicity of infection of 2 (MOI = 2) for 1 h at 4°C.…”

Transmissible Gastroenteritis Virus Infection Enhances SGLT1 and GLUT2 Expression to Increase Glucose Uptake

“…TGEV strain SHXB was isolated in Shanghai, China. The complete genome sequence for this virus is available at the GenBank database (ID number: KP202848.1) [13]. For experimental assays, cells were incubated with TGEV at a multiplicity of infection (MOI) of 2 for 1 h at 4˚C in serum-free medium and washed with phosphate-buffered saline (PBS; pH 7.2) at 4˚C three times to remove unbound virus.…”