Complete mitochondrial DNA sequence of a Conoidean gastropod, Lophiotoma (Xenuroturris) cerithiformis: Gene order and gastropod phylogeny

The mitochondrial genome of Conus textile, coxI–coxII intergenic sequences and Conoidean evolution

“…Genomic DNA was prepared from 20mg of frozen tissue from each species using the Gentra PUREGENE DNA Isolation Kit (Gentra Systems, Minneapolis, MN) according to the manufacturer's standard protocol (Sambrook and Russel, 2001). Segments of mitochondrial DNA were amplified by polymerase chain reaction (PCR) using degenerate primers described in (Bandyopadhyay et al, 2006). Primers were designed from amino acid sequences conserved in aligned mitochondrial protein and RNA sequences (Table I).…”

“…This is the first such analysis for any cone snail. As will be reported below, the gene order in the mitochondrial genome of Conus textile and of the turrid Lophiotoma cerithiformis (Powell, 1964), the only other species in the superfamily for which a complete mitochondrial DNA sequence is available (Bandyopadhyay et al, 2006), is conserved, suggesting that gene order is not useful for phylogenetic analysis of Conoidean gastropods.…”

Conservation and variation in mitochondrial genomes of gastropods Oncomelania hupensis and Tricula hortensis, intermediate host snails of Schistosoma in China

“…This variation may be considered as extremely high if at intra-species level, even though it is lower than that between genera comparisons, 15.8% and 17.4% at levels of genome and protein-coding genes, respectively for comparison between O. hupensis hupensis and T. hortensoni, and 16% and 17.5% between O. hupensis robertsoni and T. hortensis, and even lower than other comparisons (26-30%) for species in different family or order (Bandyopadhyay et al, 2006(Bandyopadhyay et al, , 2008Simison et al, 2006). In consideration of the difference in morphology and distribution (for details see Liu et al, 1981 andDavis et al, 1995), and in mitochondrial genomes as revealed in the present study, the ribbed-shelled O. hupensis hupensis and the smooth-shelled O. hupensis robertsoni may be considered as separate species, rather than sub-species.…”

“…These genes can also be fold into a tRNA-like secondary structure, except that the sequence lying between the aminoacyl and anticodon arms formed as a simple loop designated the DHU-arm replacement loop. DHU-arm replacement loop-containing tRNA ser were also found in other molluscs (Bandyopadhyay et al, 2006;Littlewood et al, 2006).…”

“…Mitochondrial (mt) genomes have been proved to be a powerful marker in resolving phylogenetic relationship among intraphylum taxa (e.g., Bandyopadhyay et al, 2006;Knudsen et al, 2006;Zarowiecki et al, 2007), and comparisons of mt genomes may provide further information in understanding the genetic difference among taxa (Boore et al, 2005;Hassanin et al, 2005). The present study was thus designed to clone the complete mt genomes of the ribbed-shelled O. hupensis hupensis, the smooth-shelled but with varix O. hupensis hupensis, and the smooth-shelled O. hupensis robertsoni, and T. hortensis.…”