Complete nucleotide sequence and genome organization of a dsRNA partitivirus infecting Pleurotus ostreatus

Lim, Won-Seok; Jeong, Ji Hye; Jeong, Rae‐Dong; Yoo, Young Bok; Yie, Se Won; Kim, Kook‐Hyung

doi:10.1016/j.virusres.2004.08.017

Cited by 53 publications

(41 citation statements)

References 26 publications

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“…2 because of the complete lack of sequences in the databases. A clade of fungal viruses assigned to the genus Partitivirus is also evident in both trees, although in this case two divergent subclades are clearly seen, as has been repeatedly noted in previous reports [1,2,5,9,25,[31][32][33]. Fungal viruses in the family Partitiviridae thus appear to be polyphyletic, and the genus Partitivirus may warrant future partition into two or more genera reflecting these subclades [9].…”

Section: Phylogenetic Relationshipssupporting

confidence: 66%

Cryspovirus: a new genus of protozoan viruses in the family Partitiviridae

et al. 2009

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The family Partitiviridae includes plant and fungal viruses with bisegmented dsRNA genomes and isometric virions in which the two genome segments are packaged separately and used as templates for semiconservative transcription by the viral polymerase. A new genus, Cryspovirus, has been approved for this family. Its name is based on that of the host genus, Cryptosporidium, which encompasses several species of apicomplexan parasites that infect a wide range of mammals, birds, and reptiles, and are a major cause of human diarrheal illness worldwide. The type species of the new genus is Cryptosporidium parvum virus 1. Distinguishing characteristics include infection of a protozoan host, a smaller capsid protein than found in other members of the family Partitiviridae, and sequence-based phylogenetic divergence.

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Section: Phylogenetic Relationshipssupporting

confidence: 66%

Cryspovirus: a new genus of protozoan viruses in the family Partitiviridae

et al. 2009

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“…OMSV from P. ostreatus with the genome size of 5.78 kbp was the first reported by Yu et al (2003). Characteristics of PeSV do not relate it to any known mycoviruses such as OMSV, OMIV, and PoV1 in P. ostreatus (Lim et al 2005), LIV in A. bisporus (Hollings 1962;Goodin et al 1992), or FvSV in Flammulina velutips. Further characterization including analysis of genome sequence will provide additional information on the nature of the virus.…”

Section: Discussionmentioning

confidence: 94%

Isolation and characterization of a novel mycovirus, PeSV, in Pleurotus eryngii and the development of a diagnostic system for it

Kang

et al. 2006

Biotechnol Lett

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A novel mycovirus was isolated from a cultivated edible mushroom, Pleurotus eryngii, with severe epidemic symptoms. Purification of the virus was carried out by a sequential procedure of polyethylene glycol precipitation, differential centrifugation, and equilibrium centrifugation in a CsCl gradient. Nuclease digestion assay and protein analysis revealed that the virus consisted of a single-stranded RNA (ssRNA) genome of 7.8 kbp which was encapsulated by a coat protein of 22 kDa. Transmission electron microscope showed that it was spherical with a diameter of 31 nm. Since there was neither a previous report on discovery of a virus in P. eryngii, nor known mushroom viruses with similar characteristics, we concluded that this is a novel virus and thus have named it as P. e ryngii Spherical Virus (PeSV). Because of a diagnostic test would be helpful in preventing the PeSV-related disease outbreaks, we developed a triple antibody sandwich-ELISA (TAS-ELISA) system using anti-PeSV mouse monoclonal and anti-PeSV rabbit polyclonal antibodies. The TAS-ELISA system successfully detected less than 0.5 microg of the virus particles in 1 g diseased mushroom tissue collected from various commercial farms.

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“…Oyster mushroom spherical virus 1 (OMSV-1, not yet classified by ICTV), has a positive-sense ssRNA genome Yu et al, 2003), and Pleurotus ostreatus virus 1 (PoV-1, family Partitiviridae, genus Partitivirus) has a dsRNA genome (Lim et al, 2005). Thus far, no clear association has EFFECTS OF VIRUSES ON FUNGAL FITNESS been found between virus infection and any adverse phenotypes in P. ostreatus.…”

Section: Diseases Of Cultivated Mushroomsmentioning

confidence: 99%