Complete nucleotide sequence of alfalfa mosaic virus RNA 4

Brederode, Frans Th.; Koper-Zwarthoff, Ellen C.; Bol, John F.

doi:10.1093/nar/8.10.2213

Cited by 98 publications

(21 citation statements)

References 19 publications

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“…Using alfalfa mosaic virus R N A (Cornelissen et al, 1983a, b;Barker et al, 1983: Brederode et al, 1980 and tobacco ringspot virus R N A (Murant et al, 1981) as standards, the molecular size of DVX R N A was estimated to be 6.0 kb (Fig. I a).…”

Section: Analysis Of D V X Rna Isolated Jrom Virus Particlesmentioning

confidence: 99%

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Guilford

Forster

1986

Journal of General Virology

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SUMMARYThe genomic RNA (6.0 kb) and five subgenomic RNAs of 4-9, 4.0, 2.1, 1.4 and 0-8 kb were detected by Northern hybridization in leaves ofNicotiana clevelandii infected with daphne virus X (DVX). Also, five species of dsRNA specific to DVX were extracted from infected leaves. In denaturing gels the dsRNAs gave bands with mobilities similar to those of the genomic RNA and the 4.9, 4.0, 2.1 and 0-8 kb subgenomic RNAs. The single-stranded RNAs apparently contain a tract of poly(A) as shown by oligo(dT)-cellulose chromatography and by an increased mobility on agarose gels following digestion with RNase H in the presence of oligo(dT)l 2-~ 8. When added to reticulocyte lysate, DVX RNA directed the synthesis of a 160000 mol. wt. (160K) protein and several smaller proteins. The coat protein (26K) was identified by immunoprecipitation with antiserum to DVX particles among the translation products of poly(A)-containing RNA isolated from infected leaves. This protein (26K) was translated from the smallest subgenomic RNA (0-8 kb) but was not translated from fulllength DVX RNA isolated by centrifugation through formamide-sucrose gradients.

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Section: Analysis Of D V X Rna Isolated Jrom Virus Particlesmentioning

confidence: 99%

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Guilford

Forster

1986

Journal of General Virology

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“…are shown; these are not necessarily a true indication of the relative sizes of 5' leader, 3' tail and intercistronic regions. The information used is given in the references in Table 3 and the following: TMV (type strain), Richards et al (1978); TYMV, ; SBMV, Ghosh et al (1981); BMV, Ahlquist et al, (1981 b); A1MV, Brederode et al (1980), Pinck et al (1981); BSMV, A. Jackson, personal communication; CPMV, Rezelman et al (1980), Franssen et al (1982 …”

Section: ' Terminal Structuresmentioning

confidence: 99%

Genome Expression of Plant Positive-strand RNA Viruses

Davies

Hull

1982

Journal of General Virology

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“…Sizes of the four ccRNAs were estimated by electrophoresis in 6~ polyacrylamide gel slabs (40 x 20 x 0.05 cm) containing 98~ formamide (Maniatis & Efstradiatis, 1980 (Spohr et al, 1976); alfalfa mosaic virus (AMV) RNA 4, 881 nucleotides (Brederode et al, 1980); yeast 5-8S RNA, 158 nucleotides (Rubin, 1973); yeast 5S RNA, 121 nucleotides (Miyazaki, 1974), Escherichia coli phenylalanine tRNA, 76 nucleotides (Barrell & Sanger, 1969).…”

Section: Source Of Infected Materialmentioning

confidence: 99%

Characterization of the Different Electrophoretic Forms of the Cadang-Cadang Viroid

Mohamed¹,

Haseloff

Imperial³

et al. 1982

Journal of General Virology

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SUMMARYThe relationship between symptom development and changes in the fast and slow electrophoretic forms of the two cadang-cadang disease-associated RNAs (ccRNA-1 and ccRNA-2) has been examined. The fast form of each is present in trees for up to 2 years before the appearance of symptoms, indicating an incubation period of about 2 years. Trees showing the first symptoms (on the nuts) contain only the fast form of the ccRNAs; the development of leaf symptoms coincides with the first detection of the slow form. After this time, both slow and fast forms are found in the newly developing fronds for the next 9 to 12 months but then, as symptoms develop, new fronds contain only the slow form. Therefore, at later stages of disease, only the slow form can be detected in all fronds. The fast form of ccRNA-1 and/or ccRNA-2 therefore appears to be the infectious form in nature. The number of nucleotides in the four ccRNAs as determined by polyacrylamide gel electrophoresis under denaturing conditions are: ccRNA-I fast, 250; ccRNA-1 slow, 300; ccRNA-2 fast, 500; ccRNA-2 slow, 600. On the basis of two-dimensional fingerprints of ribonuclease A and T1 digests of the four ccRNAs, it was concluded that the ccRNAs are composed of repeated sequences of ccRNA-I fast, and each of the ccRNA-2 forms is a dimer of the respective ccRNA-1 form.

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Complete nucleotide sequence of alfalfa mosaic virus RNA 4

Cited by 98 publications

References 19 publications

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Genome Expression of Plant Positive-strand RNA Viruses

Characterization of the Different Electrophoretic Forms of the Cadang-Cadang Viroid

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