Complete nucleotide sequence of alfalfa mosaic virus RNA 2

Cornelissen, Ben J. C.; Brederode, Frans Th.; Veenerman, Gerrit H.; Boorn, Jacques H. Van; Bol, John F.

doi:10.1093/nar/11.10.3019

Cited by 78 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mass of protein synthesized was calculated from the amount of[35S]methionine in the bands and the number of methionine residues calculated to be in each protein (i.e. 18, 16, 7 and 2 respectively for PI, P2, P3 and P4) from the sequences of RNA 1 (strain 425, Cornelissen et al, 1983a), RNA 2 (strain 425, Cornelissen et al, 1983b) and RNA 3 (S-strain, Ravelonandro et al, 1984), allowing for the removal of the N-terminal methionine residue from coat protein P4 synthesized in the wheat germ extracts (Thivent, 1983).…”

Section: Methodsmentioning

confidence: 99%

Preparation of an Antiserum against an in vitro Translation Product of Alfalfa Mosaic Virus RNA 3

Berna

Godefroy-Colburn

Stussi-Garaud

1985

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYAntisera were raised against P3 (mol. wt. 32 000), the full-length translation product of alfalfa mosaic virus (A1MV) RNA 3. P3 was made by translation in wheat germ extracts, using unfractionated AlMV RNA as message, and the products were then fractionated either by centrifugation through a sucrose gradient, or by phosphocellulose chromatography, each technique being followed by SDS-PAGE. Preparations made by each method were used in sequence to immunize a rabbit. The resulting antisera reacted on immunoblots with P3 synthesized in vitro, at dilutions of about 1 : 10000, but did not react with the translation products of A1MV RNA 1, RNA 2 or RNA 4. Although the antisera contained antibodies against some wheat germ components, most were removed by preabsorbing the antisera with the wheat germ extract. A minor wheat germ translation product of RNA 3, P'3, also reacted with the antisera. Since the mol. wt. of P'3 is apparently 1500 higher than that of P3, it is possible that P'3 is a readthrough product of the P3 cistron. With one antiserum preparation we were able to detect P3 in extracts of tobacco plants infected with AIMV.

show abstract

Section: Methodsmentioning

confidence: 99%

Preparation of an Antiserum against an in vitro Translation Product of Alfalfa Mosaic Virus RNA 3

Berna

Godefroy-Colburn

Stussi-Garaud

1985

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The RNA-2 of members of the Bromoviridae encodes a polymerase (Ahlquist et al, 1984;Cornelissen et al, 1983;Rezaian et al, 1984). As RNA dependent polymerases are probably among the most ancient of viral enzymes and appear to be more highly conserved than coat proteins, they are potentially 'the best ruler' for molecular taxonomy of RNA viruses (Bruenn, 1991).…”

mentioning

confidence: 99%

The nucleotide sequence of citrus leaf rugose ilarvirus RNA-2

Scott

1994

Journal of General Virology

View full text Add to dashboard Cite

The nucleotide sequence of citrus leaf rugose ilarvirus (CiLRV) RNA-2 consists of 2990 nucleotides and contains one open reading frame (ORF) which encodes a deduced translation product of 832 amino acids with a calculated M~. of 95501 (95K). The 5' terminus of the RNA has a mTGppp cap. Both the nucleotide sequence of CiLRV RNA-2 and its translated polypeptide share homologies with the nucleotide sequence and translated polypeptide, respectively, of RNA-2 of alfalfa mosaic virus (AlMV). The homology occurs in the central region of both the nucleic acid sequence and the polypeptide. Homologies between either CiLRV or A1MV and other Bromoviridae (cucumber mosaic virus -CMV, brome mosaic virus -BMV and cowpea chlorotic mottle virus -CCMV) were far less. Alignment of the 104 amino acid region (polymerase signature) of the 95K protein against 10 other 'alpha-like' plant viral polymerase signatures showed that CiLRV and AlMV are more closely related to each other than to CMV, BMV or CCMV. This is the first report of the full-length sequence of RNA-2 of an ilarvirus.

show abstract

“…Tobacco plants (Nicotiana tabacum L.var. Samsun NN) were inoculated with alfalfa mosaic virus, a plant virus with a tripartite genome (3) consisting in total of 8274 bases (2,5,6). We used our standard strain AMV 425 which is fully adapted to tobacco.…”

mentioning

confidence: 99%

Plant-virus-based vectors for gene transfer will be of limited use because of the high error frequency during viral RNA synthesis

1985

View full text Add to dashboard Cite

The error frequency during the RNA replication of alfalfa mosaic virus (AMV) was calculated to be significantly higher than 10(-5). It may be expected that RNA synthesis in general will have low fidelity compared to DNA synthesis. The low fidelity of RNA replication will severely restrict the usefulness of vectors for genetic engineering which are based on RNA viruses, viroids or DNA viruses which are replicated via an RNA intermediate (e.g. caulimoviruses). Spontaneous mutants selected by host shift were found to be much less stable than UV-induced mutants. This difference points to variations in fidelity during RNA synthesis, probably due to the local sequence of the template.

show abstract

Complete nucleotide sequence of alfalfa mosaic virus RNA 2

Cited by 78 publications

References 24 publications

Preparation of an Antiserum against an in vitro Translation Product of Alfalfa Mosaic Virus RNA 3

Preparation of an Antiserum against an in vitro Translation Product of Alfalfa Mosaic Virus RNA 3

The nucleotide sequence of citrus leaf rugose ilarvirus RNA-2

Plant-virus-based vectors for gene transfer will be of limited use because of the high error frequency during viral RNA synthesis

Contact Info

Product

Resources

About