Complete nucleotide sequence of chikungunya virus and evidence for an internal polyadenylation site

Khan, Afjal Hossain; Morita, Kouichi; Parquet, Maria del Carmen; Hasebe, Futoshi; Mathenge, Edward Gitau Matumbi; Igarashi, Akira

doi:10.1099/0022-1317-83-12-3075

Cited by 250 publications

(198 citation statements)

References 34 publications

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“…Complete genomic sequence of CHIKV has been determined. [5] The complete genome was found to be 11,805 nucleotides in length. Coding sequences consisting of two large open reading frames of 7422 nucleotide and 3744 nucleotide encoding the non-structural polyprotein (2474 amino acids) and the structural polyprotein (1248 amino acids), respectively.…”

mentioning

confidence: 99%

Chikungunya fever: A re-emerging viral infection

Chhabra¹,

Mittal²,

Bhattacharya³

et al. 2008

Indian J Med Microbiol

129

145

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mentioning

confidence: 99%

Chikungunya fever: A re-emerging viral infection

Chhabra¹,

Mittal²,

Bhattacharya³

et al. 2008

Indian J Med Microbiol

129

145

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“…Another ORF, with 3,735 nucleotides, is initiated by a start codon at position 7,567±7,569 and terminated by a stop codon at position 11,299±11,301. This ORF encodes for a polyprotein of 1,244 amino acids, from which 5 structural proteins (capsid -CP, E3, E2, 6K and E1) are formed after proteolytic cleavage 8,9 .…”

Section: Figueiredo Mlg and Figueiredo Ltm -Emerging Alphaviruses In mentioning

confidence: 99%

Emerging alphaviruses in the Americas: Chikungunya and Mayaro

Figueiredo

2014

Rev. Soc. Bras. Med. Trop.

124

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Chikungunya virus (CHIKV) and Mayaro virus (MAYV) are emergent arthropod-borne viruses that produce outbreaks of acute febrile illness with arthropathy. Despite their different continental origins, CHIKV and MAYV are closely related and are components of the Semliki Forest Complex of the Alphavirus (Togaviridae). MAYV and, more recently, CHIKV, which are both transmitted by Aedes mosquitoes, have resulted in severe public health problems in the Americas, including Brazil. In this review, we present aspects of the pathogenesis, clinical presentation and treatment of febrile illnesses produced by CHIKV and MAYV. We also discuss the epidemiological aspects and effects related to the prophylaxis of infections by both viruses.

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“…Structurally, the chikungunya viral genome encodes four major non-structural proteins (nsP 1-4) and three major structural proteins (A capsid and two envelope proteins) (Khan et al, 2002). Of these, the function of nsP3 is poorly understood (Han et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Towards the Identification of Novel Phytochemical Leads as Macrodomain Inhibitors of Chikungunya Virus Using Molecular Docking Approach

2017

J App Pharma Sci

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The outbreak of Chikungunya fever worldwide has raised a threat to human health and an economic burden on the affected countries. Currently, there is no cure for Chikungunya. The computational methods assist in identifying the drug leads for the target. This work reports the potential phytochemical inhibitors against a unique macro domain present in the conserved N-terminal region of Non-structural protein nsP3 of Chikungunya virus. Macro domain contributes a lot to viral fusion and replication. The shape based docking protocol was applied to predict the ligand conformations and their relative orientations with 2-ADP Ribose binding site of the target. The phytochemicals showed favorable bonded and non-bonded interactions with crucial amino acids. The binding affinity of the ligands to the target structure was estimated with six wellknown scoring functions. An all-atom molecular dynamics simulation was performed for the macro domain in apo form and the complex with the best scoring ligand to study the stability and the conformational flexibility of the complexes. Based on the multiple scoring functions, drug-like properties, amino acid interactions and molecular dynamics studies, it is believed that the phytochemicals screened may well compete with the substrate. This is turn can inhibit the function of macro domain, eventually the loss of virulence of chikungunya virus. The work also gives a room for designing novel drugs using chemoinformatics, based on the structures of phytochemical leads reported in this study.

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Complete nucleotide sequence of chikungunya virus and evidence for an internal polyadenylation site

Cited by 250 publications

References 34 publications

Chikungunya fever: A re-emerging viral infection

Chikungunya fever: A re-emerging viral infection

Emerging alphaviruses in the Americas: Chikungunya and Mayaro

Towards the Identification of Novel Phytochemical Leads as Macrodomain Inhibitors of Chikungunya Virus Using Molecular Docking Approach

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