Complete nucleotide sequence of the genomic RNA of Narcissus yellow stripe virus from Chinese narcissus in Zhangzhou city, China

The genetic diversity of narcissus viruses related to turnip mosaic virus blur arbitrary boundaries used to discriminate potyvirus species

“…One unequivocal recombination site was found in the middle of the CI coding region (near nt 4600) of the NYSV Zhangzhou sequence (NC_011541) as previously reported [ 27 ]. The genomes most closely related to the two regions of the NYSV Zhangzhou isolate are those of NY-HR38 and NY-OI1 isolates with statistical support (i.e.…”

“…TuMV is a member of a clearly defined lineage of potyviruses, the TuMV phylogenetic group, all of which, except TuMV, have been isolated from monocotyledonous plants [ 3 ]. The TuMV group consists of Japanese yam mosaic virus (JYMV) [ 19 – 21 ], narcissus late season yellows virus (NLSYV) [ 22 , 23 ], narcissus yellow stripe virus (NYSV) [ 23 – 27 ], scallion mosaic virus (ScaMV) [ 14 , 28 ], wild onion symptomless virus (WoSV) [ 13 ] and TuMV [ 11 , 12 , 29 ].…”

“…N . tazetta , a feral, is known to be infected with several different potyviruses including NYSV, which was the first narcissus virus to be described [ 24 – 26 , 31 ], and also NLSYV, Indian narcissus virus, lily mottle virus, narcissus degeneration virus (NDV), ornithogalum mosaic virus, cyrtanthus elatus virus A (CyEVA) and iris severe mosaic virus [ 15 , 22 , 27 , 32 – 35 ]. Early attempts to resolve this complex proved to be difficult [ 36 ] before enzyme-linked immunosorbent assay (ELISA) tests were used.…”

The ‘emergence’ of turnip mosaic virus was probably a ‘gene-for-quasi-gene’ event

“…TuMV has a wide natural and experimental host range among dicots, especially brassicas, whereas TuMV-OM infects orchids and, in experiments, a few dicots, but not brassicas. TuMV and TuMV-OM, together with several viruses of monocots, form the TuMV group; Japanese yam mosaic virus [11], scallion mosaic virus [12], narcissus yellow stripe virus [13], narcissus late season yellows virus [14,15], all known from complete genomic sequences, and a seventh, Indian narcissus potyvirus-isolate 2 Lucknow [16], which is known from a partial genomic sequence. Thus TuMV is the only species of the TuMV group known to infect dicots in nature, all the others infect monocots.…”

First Genome Sequence of Wild Onion Symptomless Virus, a Novel Member of Potyvirus in the Turnip Mosaic Virus Phylogenetic Group

“…The complete two genome sequences (only three mismatches), excluding a 25-nt 5′-Tu5T5P primer sequence used for amplifying the genome, were 9,369 nt long. The polyprotein sequence comparisons using EMBOSS Needle ( http://www.ebi.ac.uk/Tools/psa/emboss_needle/ ) between the WoSV-TUR256-1 and TUR256-2 sequences and TuMV group virus sequences, TuMV (accession numbers AB701690 and AB093598) ( 8 , 9 ), NLSYV (accession numbers JQ326210, NC_023628, and JX156421) ( 10 , 11 ), NYSV (accession numbers NC_011541, JQ911732, and JQ395042) ( 12 , 13 ), ScaMV (accession no. NC_003399) ( 14 ), and JYMV (accession numbers KJ789140, KJ701427, NC_000947, and AB016500) ( 15 – 17 ), showed 60 to 67% nucleotide identities.…”