Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA

Carattoli, Alessandra; Aschbacher, Richard; March, Albert; Larcher, Clara; Livermore, David M.; Woodford, Neil

doi:10.1093/jac/dkq269

Cited by 62 publications

(55 citation statements)

References 12 publications

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“…In addition, combinations of fosfomycin with meropenem, colistin, and gentamicin prevented the development of resistance to fosfomycin in 69.2, 53.8, and 81.8% of examined isolates, respectively. Similar results were obtained by another study that demonstrated synergy of fosfomycin with imipenem, meropenem, doripenem, colistin, netilmicin, and tigecycline for 74,70,74,36,42, and 30% of 50 KPC-producing K. pneumoniae isolates, respectively (228).…”

Section: In Vitro Synergysupporting

confidence: 87%

“…Indeed, insertion elements (ISs) such as IS26, ISEc33, ISSen4, and ISAba125, either alone or as parts of transposons (e.g., Tn3 and Tn1696), commonly flank M␤L-encoding regions (Fig. 1, structures II to VI) (42,59,114,165,167,215,218,273). At least some of the latter elements apparently participate in the spread of M␤L-encoding sequences among different genetic units.…”

Section: M␤lsmentioning

confidence: 99%

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Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions

et al. 2012

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SUMMARY The spread of Enterobacteriaceae , primarily Klebsiella pneumoniae , producing KPC, VIM, IMP, and NDM carbapenemases, is causing an unprecedented public health crisis. Carbapenemase-producing enterobacteria (CPE) infect mainly hospitalized patients but also have been spreading in long-term care facilities. Given their multidrug resistance, therapeutic options are limited and, as discussed here, should be reevaluated and optimized. Based on susceptibility data, colistin and tigecycline are commonly used to treat CPE infections. Nevertheless, a review of the literature revealed high failure rates in cases of monotherapy with these drugs, whilst monotherapy with either a carbapenem or an aminoglycoside appeared to be more effective. Combination therapies not including carbapenems were comparable to aminoglycoside and carbapenem monotherapies. Higher success rates have been achieved with carbapenem-containing combinations. Pharmacodynamic simulations and experimental infections indicate that modification of the current patterns of carbapenem use against CPE warrants further attention. Epidemiological data, though fragmentary in many countries, indicate CPE foci and transmission routes, to some extent, whilst also underlining the lack of international collaborative systems that could react promptly and effectively. Fortunately, there are sound studies showing successful containment of CPE by bundles of measures, among which the most important are active surveillance cultures, separation of carriers, and assignment of dedicated nursing staff.

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Section: In Vitro Synergysupporting

confidence: 87%

Section: M␤lsmentioning

confidence: 99%

Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions

et al. 2012

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“…The same reading frame was also detected on the IncN plasmid R46 (NC_003292). Immediately upstream of this open reading frame, an incomplete reading frame for a protein was detected, of which the C-terminal 186 aa corresponded exactly to the Mrr protein of the IncN plasmid pKOX105 from Klebsiella oxytoca (7).…”

Section: Resultsmentioning

confidence: 99%

Analysis of bla _CTX-M -Carrying Plasmids from Escherichia coli Isolates Collected in the BfT-GermVet Study

Schink

Kadlec

Schwarz

2011

Appl Environ Microbiol

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In this study, 417 Escherichia coli isolates from defined disease conditions of companion and farm animals collected in the BfT-GermVet study were investigated for the presence of extended-spectrum ␤-lactamase (ESBL) genes. Three ESBL-producing E. coli isolates were identified among the 100 ampicillin-resistant isolates. The E. coli isolates 168 and 246, of canine and porcine origins, respectively, harbored bla CTX-M-1 , and the canine isolate 913 harbored bla CTX-M-15 , as confirmed by PCR and sequence analysis. The isolates 168 and 246 belonged to the novel multilocus sequence typing (MLST) types ST1576 and ST1153, respectively, while isolate 913 had the MLST type ST410. The ESBL genes were located on structurally related IncN plasmids in isolates 168 and 246 and on an IncF plasmid in isolate 913. The bla CTX-M-1 upstream regions of plasmids pCTX168 and pCTX246 were similar, whereas the downstream regions showed structural differences. The genetic environment of the bla CTX-M-15 gene on plasmid pCTX913 differed distinctly from that of both bla CTX-M-1 genes. Detailed sequence analysis showed that the integration of insertion sequences, as well as interplasmid recombination events, accounted for the structural variability in the bla CTX-M gene regions.

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“…Whole-plasmid sequencing and comparative genomic analyses have identified common functional modules and unique acquired genes that provide important molecular information for developing epidemiological tools to identify and track the spread of resistance plasmids within and among Enterobacteriaceae species (17)(18)(19)(20). Various bla KPC-2 -and bla KPC-3 -harboring plasmids belonging to different Inc groups have been successfully sequenced, including IncN plasmids p9 and p12 (10), IncFII plasmids pKPHS2 (21), pKP048 (19), pKpQIL (20), pKpQIL-IT (18), and pSLMT (HQ589350), and ColE1 plasmid p15S (10).…”

mentioning

confidence: 99%

Complete Nucleotide Sequences of bla _KPC-4 - and bla _KPC-5 -Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey

Chen

Chavda

Fraimow

et al. 2013

Antimicrob Agents Chemother

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h Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have emerged as major nosocomial pathogens. bla KPC , commonly located on Tn4401, is found in Gram-negative bacterial strains, with the two most common variants, bla KPC-2 and bla KPC-3 , identified in plasmids with diverse genetic backgrounds. In this study, we examined bla KPC-4 -and bla KPC-5 -bearing plasmids recovered from two K. pneumoniae strains, which were isolated from a single New Jersey hospital in 2005 and 2006, respectively. IncN plasmid pBK31551 is 84 kb in length and harbors bla KPC-4 , bla TEM-1 , qnrB2, aac(3)-Ib, aph(3=)-I, qacF, qacE⌬1, sul1, and dfrA14, which confer resistance to ␤-lactams, quinolones, aminoglycosides, quaternary ammonium compounds, and co-trimoxazole. The conserved regions within pBK31551 are similar to those of other IncN plasmids. Surprisingly, analysis of the Tn4401 sequence revealed a large IS110-and Tn6901-carrying element (8.3 kb) inserted into the istA gene, encoding glyoxalase/bleomycin resistance, alcohol dehydrogenase, and S-formylglutathione hydrolase. Plasmid pBK31567 is 47 kb in length and harbors bla KPC-5 , dfrA5, qacE⌬1, and sul1. pBK31567 belongs to a novel IncX subgroup (IncX5) and possesses a highly syntenic plasmid backbone like other IncX plasmids; however, sequence similarity at the nucleotide level is divergent. The bla KPC-5 gene is carried on a Tn4401 element and differs from the genetic environment of bla KPC-5 described in Pseudomonas aeruginosa strain P28 from Puerto Rico. This study underscores the genetic diversity of multidrug-resistant plasmids involved in the spread of bla KPC genes and highlights the mobility and plasticity of Tn4401. Comparative genomic analysis provides new insights into the evolution and dissemination of KPC plasmids belonging to different incompatibility groups.

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Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA

Cited by 62 publications

References 12 publications

Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions

Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions

Analysis of bla _CTX-M -Carrying Plasmids from Escherichia coli Isolates Collected in the BfT-GermVet Study

Complete Nucleotide Sequences of bla _KPC-4 - and bla _KPC-5 -Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey

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Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA

Cited by 62 publications

References 12 publications

Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions

Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions

Analysis of bla CTX-M -Carrying Plasmids from Escherichia coli Isolates Collected in the BfT-GermVet Study

Complete Nucleotide Sequences of bla KPC-4 - and bla KPC-5 -Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey

Contact Info

Product

Resources

About

Analysis of bla _CTX-M -Carrying Plasmids from Escherichia coli Isolates Collected in the BfT-GermVet Study

Complete Nucleotide Sequences of bla _KPC-4 - and bla _KPC-5 -Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey