Complete Nucleotide Sequence of Two Multidrug-Resistant IncR Plasmids from Klebsiella pneumoniae

Abstract

fWe report here the complete nucleotide sequence of two IncR replicons encoding multidrug resistance determinants, including ␤-lactam (bla DHA-1 , bla SHV-12 ), aminoglycoside (aphA1, strA, strB), and fluoroquinolone (qnrB4, aac6=-1b-cr) resistance genes. The plasmids have backbones that are similar to each other, including the replication and stability systems, and contain a wide variety of transposable elements carrying known antibiotic resistance genes. This study confirms the increasing clinical importance of IncR replicons as resistance gene carriers.

Complete Sequence of a Novel IncR-F33:A–:B– Plasmid, pKP1034, Harboring fosA3 , bla KPC-2 , bla CTX-M-65 , bla SHV-12 , and rmtB from an Epidemic Klebsiella pneumoniae Sequence Type 11 Strain in China

“…However, bla SHV-12 instead of bla SHV-11 is identified in pKP1034. bla SHV-12 is carried by an IS26-flanked composite transposon, as previously reported (24,25). Nonetheless, the IS26 adjacent to Tn1721 was truncated, probably by Tn1721, at the same location as its counterpart in pKPC-LK30.…”

Third-Generation-Cephalosporin-Resistant Klebsiella pneumoniae Isolates from Humans and Companion Animals in Switzerland: Spread of a DHA-Producing Sequence Type 11 Clone in a Veterinary Setting

“…The bla DHA-1 and bla CTX-M-1 genes from animal isolates were associated with nonconjugative R plasmids. R plasmids are commonly found in K. pneumoniae isolates (17) and play an important role as collectors of resistance genes in both human and companion animal settings (2,3,17,18). The bla CMY-2 gene detected in ST1463 isolates was linked to I1 plasmids belonging to ST2 as determined by plasmid MLST (pMLST) (19), a combination already observed in E. coli from dogs in Denmark (20).…”

WITHDRAWN: Structure genomics of two chimera plasmids p675920-1 and p675920-2 coexisting in a multi-drug resistant Klebsiella pneumoniae isolate

“…The IncR replicon was initially discovered in 2009, and often coexists as an auxiliary replicon with other replicons such as IncA/C, IncF, and IncH [9], constituting multi-replicon plasmids. The IncR replicon alone can promote plasmid replication, and IncR plasmids are herein designated as those containing the sole IncR replicon.…”

“…The core backbone regions of IncR plasmids are composed of genes or gene loci responsible for plasmid replication initiation (repB) and maintenance (parAB, umuCD, vagCD, and resD) but lack conjugal transfer regions, and thus IncR plasmids are not self-transmissible [10]. An array of IncR plasmids, such as pEFER (IncR reference plasmid, accession number CU928144), pKPS30 (accession number KF793937) [9], pKPS77 (accession number KF954150) [9] , pKP1780 (accession number JX424614) [11] and pKPC-LK30 (accession number KC405622) [12], have been fully sequenced. IncR plasmids have been increasingly reported in Enterobacteriaceae species, and carry various kinds of antibiotic resistance genes.…”