Complete Nucleotide Sequences of S1 and N Genes of Infectious Bronchitis Virus Isolated in Japan and Taiwan

Shieh, Happy K.; Shien, Jui-Hung; Chou, Ho-Yen; Shimizu, Yukiomi; Chen, Jen‐Ni; Chang, Poa-Chun

doi:10.1292/jvms.66.555

Cited by 24 publications

(29 citation statements)

References 11 publications

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“…However it is necessary to confirm the intergenic recombination event by sequencing larger gene segments, as extensively reported (Estevez et al, 2003;Huang et al, 2004;Shieh et al, 2004;Jackwood et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Molecular studies of the Brazilian infectious bronchitis virus isolates

Abreu

Mourão

Santos

et al. 2010

Rev. Bras. Cienc. Avic.

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Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).

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Section: Resultsmentioning

confidence: 99%

Molecular studies of the Brazilian infectious bronchitis virus isolates

Abreu

Mourão

Santos

et al. 2010

Rev. Bras. Cienc. Avic.

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“…So far, based on phylogenetic analysis of the S1 and N genes, genetic recombinants of IBV were suspected to exist in several countries including Japan [1, 8,21,27]. Moreover, based on phylogenetic analysis of several regions of the S2 gene, the Japanese KB8523 strain [22] was considered to be a potential recombinant [12].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Avian Infectious Bronchitis Viruses in Japan Based on Analysis of S2 Glycoprotein Gene

Mase

Inoue

Yamaguchi

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. To understand the genetic diversity of the S2 gene of infectious bronchitis viruses (IBV) isolated in Japan, we determined the nucleotide sequences of these IBVs using the reverse transcriptase polymerase chain reaction method coupled with direct sequencing. IBV isolated in Japan were classified into six different groups by phylogenetic analysis based on the S2 gene. However, the classification based on the S2 gene of IBV isolated in Japan was different for some of the strains from those obtained with our previous analysis of the S1 gene. This suggested that genetic recombination between the virus strains classified into different genetic groups had occurred in poultry, and that recombinant viruses might be epidemic in Japan. KEY WORDS: diversity, infectious bronchitis virus, S2 gene.

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“…Meanwhile, Zwaagstra et al [8] reported that the N gene is highly conserved among different IBV isolates. Nevertheless, analysis based on S1 and N gene sequences may provide valuable information on the evolutionary of IBV [9][10][11]. Hence, this study focus on the molecular characterization and phylogenetic analysis of the structural (S1, S2, M, and N) genes of two Malaysian IBV isolates, MH5365/95 and V9/04.…”

Section: Introductionmentioning

confidence: 99%

Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia

2009

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Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region of both IBV isolates. Phylogenetic analysis of S1 gene and sequences analysis of M gene indicated that MH5365/95 and V9/04 belong to non-Massachusetts strain. However, both isolates share only 77% identity. Analysis based on S1 gene showed that MH5365/95 shared more than 87% identity to several Chinese strains. Meanwhile, V9/04 showed only 67-77% identity to all the previously studied IBV strains included in this study suggesting it is a variant of IBV isolate that is unique to Malaysia. Phylogenetic analysis suggests, although both isolates were isolated 10 years apart from different states in Malaysia, they shared a common origin. Analysis based on S2 and N genes indicated that both strains are highly related to each other, and there are fewer mutations which occurred in the respective genes.

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Complete Nucleotide Sequences of S1 and N Genes of Infectious Bronchitis Virus Isolated in Japan and Taiwan

Cited by 24 publications

References 11 publications

Molecular studies of the Brazilian infectious bronchitis virus isolates

Molecular studies of the Brazilian infectious bronchitis virus isolates

Genetic Diversity of Avian Infectious Bronchitis Viruses in Japan Based on Analysis of S2 Glycoprotein Gene

Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia

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