Complete polarization state generator with one variable retarder and its application for fast and sensitive measuring of two-dimensional birefringence distribution

Shribak, Michael

doi:10.1364/josaa.28.000410

Cited by 33 publications

(20 citation statements)

References 25 publications

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“…For correction of the background gradient vector field it is possible to employ special procedure, which mathematical description we published early [34, 35]. The procedure is based on specimen images, which are recorded with no structures in the optical path.…”

Section: Data Processingmentioning

confidence: 99%

Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation

Shribak¹

2013

J. Opt. Soc. Am. A

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We describe quantitative orientation-independent differential interference contrast (OI-DIC) microscope, which allows the bias retardation to be modulated and shear directions to be switched rapidly without any mechanically moving. The shear direction is switched by regular liquid crystal cell sandwiched between two standard DIC prisms. Another liquid crystal cell modulates the bias. Techniques for measuring parameters of DIC prisms and calibrating the bias are shown. Two sets of raw DIC images with the orthogonal shear directions are captured within a second. Then the quantitative image of optical path gradient distribution within a thin optical section is computed. The gradient data are used to obtain quantitative distribution of optical path, which represents refractive index gradient or height distribution. Computing enhanced regular DIC images with any desired shear direction is also possible.

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Section: Data Processingmentioning

confidence: 99%

Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation

Shribak¹

2013

J. Opt. Soc. Am. A

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“…Later, Shribak proposed the SLC-polscope that employs an alternative universal compensator (also called complete polarization state generator) that is built from a single LC-based variable retarder (the LC-PolScope employs two LC retarders), a rotatable linear polarizer and a quarter wave film. (Shribak, 2011). The SLC-polscope uses a new 3-frame algorithm.…”

Section: Enhancing Polarized Light Microscopy Using Liquid Crystal Dementioning

confidence: 99%

Living Cells and Dynamic Molecules Observed with the Polarized Light Microscope: the Legacy of Shinya Inoué

Tani

Shribak

Oldenbourg

2016

The Biological Bulletin

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In 1948, Shinya Inoué arrived in the United States for graduate studies at Princeton. A year later he came to Woods Hole, starting a long tradition of summer research at MBL, which quickly became Inoué’s scientific home. Primed by his Japanese mentor Katsuma Dan, Inoué followed Dan’s mantra to work with healthy living cells, on a fundamental problem (mitosis), with a unique tool set that he refined for precise and quantitative observations (polarized light microscopy), and a fresh and brilliant mind that was unafraid of challenging current dogma. Building on this potent combination, Inoué contributed landmark observations and concepts in cell biology, including the notion of dynamic fine structures inside living cells in which molecular assemblies such as mitotic spindle fibers exist in a delicate equilibrium with their molecular building blocks suspended in the cytoplasm. In the late 1970s and 80s, Inoué and others at the MBL were instrumental in conceiving of video microscopy, a groundbreaking technique that married light microscopy and electronic imaging and ushered in a revolution in how we know and what we know about living cells and the molecular mechanisms of life. This article recounts some of Inoué’s accomplishments and how his legacy has shaped current activities in polarized light imaging at the MBL.

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“…These schemes invariably involve the traditional compensator, which is either rotated under computer control (Glazer et al 1996) or replaced by electro-optical modulators, such as Pockel-cells (Allen et al 1963), Faraday rotators (Kuhn et al 2001), and liquid crystal variable retarders (Laude-Boulesteix et al 2004; Oldenbourg and Mei 1995; Shribak 2011). Alternatively, one can split the imaging beam and simultaneously capture several images, analyzing each for a different polarization state (Kaminsky et al 2007; Shribak et al 2006).…”

Section: The Lc-polscopementioning

confidence: 99%

Polarized light imaging of birefringence and diattenuation at high resolution and high sensitivity

Mehta

Shribak

Oldenbourg

2013

J. Opt.

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108

103

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Polarized light microscopy provides unique opportunities for analyzing the molecular order in man-made and natural materials, including biological structures inside living cells, tissues, and whole organisms. 20 years ago, the LC-PolScope was introduced as a modern version of the traditional polarizing microscope enhanced by liquid crystal devices for the control of polarization, and by electronic imaging and digital image processing for fast and comprehensive image acquisition and analysis. The LCPolScope is commonly used for birefringence imaging, analyzing the spatial and temporal variations of the differential phase delay in ordered and transparent materials. Here we describe an alternative use of the LC-PolScope for imaging the polarization dependent transmittance of dichroic materials. We explain the minor changes needed to convert the instrument between the two imaging modes, discuss the relationship between the quantities measured with either instrument, and touch on the physical connection between refractive index, birefringence, transmittance, diattenuation, and dichroism.

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Complete polarization state generator with one variable retarder and its application for fast and sensitive measuring of two-dimensional birefringence distribution

Cited by 33 publications

References 25 publications

Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation

Quantitative orientation-independent differential interference contrast microscope with fast switching shear direction and bias modulation

Living Cells and Dynamic Molecules Observed with the Polarized Light Microscope: the Legacy of Shinya Inoué

Polarized light imaging of birefringence and diattenuation at high resolution and high sensitivity

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