Complete pulpodentin complex regeneration by modulating the stiffness of biomimetic matrix

Qu, Tiejun; Jing, Junjun; Ren, Yinshi; Ma, Chi; Feng, Jian Q.; Yu, Qing; Liu, Xiaohua

doi:10.1016/j.actbio.2015.01.029

Cited by 78 publications

(55 citation statements)

References 48 publications

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“…This may provide a promising new therapeutic strategy for dentin-pulp tissue engineering for future endodontic treatment. Additionally, DPSCs seeded in a new developed S-scaffold which integrate the low and high-stiffness gelatin matrices into a single scaffold has been demonstrated to successfully regenerate pulp-dentin like tissue48. Therefore, further in-depth investigation shall be performed to evaluate the effect of IFN-γ induced DPSCs in the new developed S-scaffold.…”

Section: Discussionmentioning

confidence: 99%

“…NF-gelatin scaffolds with a porosity of 96.5 ± 0.2% and macropores ranging from 250 to 420 μm were kindly provided by Dr. Tiejun Qu as described previously48.…”

Section: Methodsmentioning

confidence: 99%

“…After decalcification in 17% ethylenediamine tetra-acetic acid, the samples were processed for histological observation by H&E, Masson’s trichrome and Van Gieson staining as previously described48.…”

Section: Methodsmentioning

confidence: 99%

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IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

Jiang

Luo

et al. 2017

Sci Rep

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During caries, dental pulp expresses a range of pro-inflammatory cytokines in response to the infectious challenge. Interferon gamma (IFN-γ) is a dimerized soluble cytokine, which is critical for immune responses. Previous study has demonstrated that IFN-γ at relative high concentration (100 ng/mL) treatment improved the impaired dentinogenic and immunosuppressive regulatory functions of disease-derived dental pulp stem cells (DPSCs). However, little is known about the regulatory effects of IFN-γ at relative low concentration on healthy DPSC behavior (including proliferation, migration, and multiple-potential differentiation). Here we demonstrate that IFN-γ at relatively low concentrations (0.5 ng/mL) promoted the proliferation and migration of DPSCs, but abrogated odonto/osteogenic differentiation. Additionally, we identified that NF-κB and MAPK signaling pathways are both involved in the process of IFN-γ-regulated odonto/osteogenic differentiation of DPSCs. DPSCs treated with IFN-γ and supplemented with pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) or SB203580 (a MAPK inhibitor) showed significantly improved potential for odonto/osteogenic differentiation of DPSCs both in vivo and in vitro. These data provide important insight into the regulatory effects of IFN-γ on the biological behavior of DPSCs and indicate a promising therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.

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Section: Discussionmentioning

confidence: 99%

“…NF-gelatin scaffolds with a porosity of 96.5 ± 0.2% and macropores ranging from 250 to 420 μm were kindly provided by Dr. Tiejun Qu as described previously48.…”

Section: Methodsmentioning

confidence: 99%

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IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

Jiang

Luo

et al. 2017

Sci Rep

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“…In the cell transplantation, the stem cells are isolated, cultivated in vitro, increased in number, inserted in soft scaffolds or hydrogels with or without addition of signaling molecules and calcium-phosphate nanoparticles and at last implanted in the receiving site, the empty and sterile endodontic space [140,141,142,143,144,145,146,147,148,149,150,151,152]. Instead, in the cell homing, where it is not necessary to isolate and manipulate in vitro stem cells, it is tried to reach pulpodentinal complex regeneration through the stimulation of residual stem cells by means of molecules veicolate by hydrogels [153,154,155].…”

Section: Experimental Studies In Dentistry With the Use Of Nanomatmentioning

confidence: 99%

Nanomaterials for Tissue Engineering In Dentistry

et al. 2016

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The tissue engineering (TE) of dental oral tissue is facing significant changes in clinical treatments in dentistry. TE is based on a stem cell, signaling molecule, and scaffold triad that must be known and calibrated with attention to specific sectors in dentistry. This review article shows a summary of micro- and nanomorphological characteristics of dental tissues, of stem cells available in the oral region, of signaling molecules usable in TE, and of scaffolds available to guide partial or total reconstruction of hard, soft, periodontal, and bone tissues. Some scaffoldless techniques used in TE are also presented. Then actual and future roles of nanotechnologies about TE in dentistry are presented.

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“…[21] Briefly, the total RNA was extracted using RNeasy plus MiniKit (Qiangen, USA) and first-strand cDNA was reverse-transcribed with a QuantiTect Rev. Transcription Kit (Qiangen, USA).…”

Section: Methodsmentioning

confidence: 99%

Hierarchical Nanofibrous Microspheres with Controlled Growth Factor Delivery for Bone Regeneration

Jing

Sun

et al. 2015

Adv Healthcare Materials

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The integration of controlled growth factor delivery and biomimetic architecture into a microsphere is a challenging but attractive strategy for developing new injectable biomaterials for tissue engineering. In this work, we developed a unique hierarchical nanosphere-encapsulated-in-microsphere scaffolding system for bone tissue regeneration. First, we synthesized heparin-conjugated gelatin (HG) that provides binding domains for bone morphogenetic protein 2 (BMP2) to stabilize this growth factor, protect it from denaturation and proteolytic degradation, and subsequently prolong its sustained release. Next, we developed a unique approach that includes a water-in-oil-in-oil (W/O/O) double emulsion process and a thermally induced phase separation to encapsulate BMP2-binding HG nanospheres into nanofibrous microspheres. The nanofibrous microsphere was self-assembled from synthetic nanofibers, and had superior surface area, high porosity, low density, and was an excellent carrier to support cell adhesion and tissue in-growth. BMP2 in the hierarchical microsphere was released in a multiple-controlled manner (by the binding with heparin and encapsulation of the nanosphere and microsphere) and retained its high bioactivity. An in vivo calvarial defect model confirmed that this unique hierarchical microsphere was an excellent osteoinductive scaffold for enhanced bone regeneration. By choosing different growth factors, this hierarchical microsphere system can be easily applied to other types of tissue regeneration. Our work expands the ability to develop new injectable biomaterials for advanced regenerative therapies.

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Complete pulpodentin complex regeneration by modulating the stiffness of biomimetic matrix

Cited by 78 publications

References 48 publications

IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

Nanomaterials for Tissue Engineering In Dentistry

Hierarchical Nanofibrous Microspheres with Controlled Growth Factor Delivery for Bone Regeneration

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