Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome

Thumbi, David; Eveleigh, Robert; Lucarotti, Christopher J.; Lapointe, Renée; Graham, Robert I.; Pavlik, Lillian; Lauzon, Hilary A. M.; Arif, Basil M.

doi:10.3390/v3112301

Cited by 15 publications

(13 citation statements)

References 103 publications

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“…Most baculovirus genomes sequenced to date contain from 1 to 16 hrs [50]. These DNA elements are interspersed throughout the genome and have been implicated as putative origins of DNA replication ( oris) , as enhancers of gene transcription, and in homologous recombination [51].…”

Section: Resultsmentioning

confidence: 99%

Comparative Genome Sequence Analysis of Choristoneura occidentalis Freeman and C. rosaceana Harris (Lepidoptera: Tortricidae) Alphabaculoviruses

et al. 2013

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The complete genome sequences of Choristoneura occidentalis and C. rosaceana nucleopolyhedroviruses (ChocNPV and ChroNPV, respectively) (Baculoviridae: Alphabaculovirus) were determined and compared with each other and with those of other baculoviruses, including the genome of the closely related C. fumiferana NPV (CfMNPV). The ChocNPV genome was 128,446 bp in length (1147 bp smaller than that of CfMNPV), had a G+C content of 50.1%, and contained 148 open reading frames (ORFs). In comparison, the ChroNPV genome was 129,052 bp in length, had a G+C content of 48.6% and contained 149 ORFs. ChocNPV and ChroNPV shared 144 ORFs in common, and had a 77% sequence identity with each other and 96.5% and 77.8% sequence identity, respectively, with CfMNPV. Five homologous regions (hrs), with sequence similarities to those of CfMNPV, were identified in ChocNPV, whereas the ChroNPV genome contained three hrs featuring up to 14 repeats. Both genomes encoded three inhibitors of apoptosis (IAP-1, IAP-2, and IAP-3), as reported for CfMNPV, and the ChocNPV IAP-3 gene represented the most divergent functional region of this genome relative to CfMNPV. Two ORFs were unique to ChocNPV, and four were unique to ChroNPV. ChroNPV ORF chronpv38 is a eukaryotic initiation factor 5 (eIF-5) homolog that has also been identified in the C. occidentalis granulovirus (ChocGV) and is believed to be the product of horizontal gene transfer from the host. Based on levels of sequence identity and phylogenetic analysis, both ChocNPV and ChroNPV fall within group I alphabaculoviruses, where ChocNPV appears to be more closely related to CfMNPV than does ChroNPV. Our analyses suggest that it may be appropriate to consider ChocNPV and CfMNPV as variants of the same virus species.

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Section: Resultsmentioning

confidence: 99%

Comparative Genome Sequence Analysis of Choristoneura occidentalis Freeman and C. rosaceana Harris (Lepidoptera: Tortricidae) Alphabaculoviruses

et al. 2013

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“…Five representative baculoviruses were chosen for the comparative study of SujuNPV: Autographa californica MNPV (AcMNPV, group I alphabaculovirus) [25] , Helicoverpa armigera SNPV (HearNPV, group II alphabaculovirus) [26] , Cydia pomonella GV (CpGV, betabaculovirus), Neodiprion lecontei NPV (NeleNPV, gammabaculovirus) and Culex nigripalpus NPV (CuniNPV, deltabaculovirus). SujuNPV shared 102 ORFs with AcMNPV, 108 with HearNPV, 78 with CpGV, 43 with NeleNPV, and 39 with CuniNPV, with an average amino acid (aa) identity of 36.0%, 39.0%, 28.4%, 23.0% and 16.3%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Genomic Sequencing and Analysis of Sucra jujuba Nucleopolyhedrovirus

Yin

Zhu

et al. 2014

PLoS ONE

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The complete nucleotide sequence of Sucra jujuba nucleopolyhedrovirus (SujuNPV) was determined by 454 pyrosequencing. The SujuNPV genome was 135,952 bp in length with an A+T content of 61.34%. It contained 131 putative open reading frames (ORFs) covering 87.9% of the genome. Among these ORFs, 37 were conserved in all baculovirus genomes that have been completely sequenced, 24 were conserved in lepidopteran baculoviruses, 65 were found in other baculoviruses, and 5 were unique to the SujuNPV genome. Seven homologous regions (hrs) were identified in the SujuNPV genome. SujuNPV contained several genes that were duplicated or copied multiple times: two copies of helicase, DNA binding protein gene (dbp), p26 and cg30, three copies of the inhibitor of the apoptosis gene (iap), and four copies of the baculovirus repeated ORF (bro). Phylogenetic analysis suggested that SujuNPV belongs to a subclade of group II alphabaculovirus, which differs from other baculoviruses in that all nine members of this subclade contain a second copy of dbp.

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“…Persentase kerusakan daun kelapa sawit akibat serangan Orgyia sp. (Thumbi, et al, 2011). OrleNPV ini juga, kedepan dapat digunakan sebagai salah satu agens hayati yang dapat dikembangkan untuk pengendalian hama Orgyia sp.…”

Section: Gejala Serangan Dan Kerusakan Tanamanunclassified

Ulat Bulu Orgyia sp. (Lepidoptera: Erebidae), Hama Potensial pada Tanaman Kelapa Sawit [Hairy Caterpillar, Orgyia sp. (Lepidoptera: Erebidae), Potential Pest on Oil Palm]

Hosang¹,

Alouw²,

Djufry

2017

Bull. Palma

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ABSTRAKLedakan serangan hama ulat bulu pada tanaman kelapa sawit, cukup parah di salah satu perkebunan sawit swasta di Papua Barat terjadi pada tahun 2016. Jenis ulat bulu dan tingkat kerusakannya belum diketahui secara pasti. Penelitian ini bertujuan untuk mengidentifikasi jenis hama ulat bulu, tingkat kerusakan, populasi hama, dan musuh alaminya. Survei hama dilakukan di tiga lokasi di Distrik Marmare, Kabupaten Manokwari, Papua Barat. Pada masing-masing lokasi dipilih 30 pohon contoh secara acak pada lokasi serangan hama kemudian diestimasi tingkat kerusakan. Hasil identifikasi menunjukkan bahwa jenis ulat yang menyerang tanaman kelapa sawit muda di PT Yongjing Investindo, Papua Barat adalah ulat bulu Orgyia sp. Dari 90 tanaman contoh, kerusakan ringan (5-20%) akibat serangan Orgyia sp. dapat mencapai 81,1% (73 tanaman) dan sebanyak 18,9% (17 tanaman), termasuk tingkat serangan sedang (30-40%). Serangan ulat bulu Orgyia sp. pada perkebunan sawit masih terbatas di Distrik Marmare, tetapi berpotensi meluas ke areal pertanaman sawit lainnya. Oleh karena itu, perlu dilakukan tindakan monitoring dan pengendalian di lapangan untuk mencegah kerusakan lebih parah. Kata kunci: Kelapa sawit, Orgyia. kerusakan tanaman. ABSTRACTThe outbreaks of hairy caterpillar pests on oil palm plants that are quite severe in one of the estate oil palm plantations in West Papua occurred in 2016. Species of hairy caterpillar and the level of its damage are not known yet for certain. The purposes of this research were to identify the pest causing oil palm damage and to determine the level of palm damage, pest population and their natural enemies. Three locations were selected on the Marmare Sub District, Manokwari, West Papua. In each location, 30 plants were selected randomly in the area of the pest attack and the leaf damage was estimated. Identification result of the pest that attack young oil palm trees in PT Yongjing Investindo, West Papua was the hairy caterpillar called tusock moth, Orgyia sp. The pest caused low (5-20%) and moderate (30-40%) level of foliar damage found in 81.1% (73 plants) and 18.9% (17 plants) palm population respectively. Since palm damage potentially reduced oil palm production, regularly monitoring pest populations are needed to prevent pest outbreak.

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Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome

Cited by 15 publications

References 103 publications

Comparative Genome Sequence Analysis of Choristoneura occidentalis Freeman and C. rosaceana Harris (Lepidoptera: Tortricidae) Alphabaculoviruses

Comparative Genome Sequence Analysis of Choristoneura occidentalis Freeman and C. rosaceana Harris (Lepidoptera: Tortricidae) Alphabaculoviruses

Genomic Sequencing and Analysis of Sucra jujuba Nucleopolyhedrovirus

Ulat Bulu Orgyia sp. (Lepidoptera: Erebidae), Hama Potensial pada Tanaman Kelapa Sawit [Hairy Caterpillar, Orgyia sp. (Lepidoptera: Erebidae), Potential Pest on Oil Palm]

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