Complete Sequence and Enhancer Function of the Homologous DNA Regions of
            <i>Autographa californica</i>
            Nuclear Polyhedrosis Virus

Guarino, Linda A.; Gonzalez, Mark A.; Summers, Max D.

doi:10.1128/jvi.60.1.224-229.1986

Cited by 163 publications

(60 citation statements)

References 35 publications

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“…Most baculoviral genomes contain homologous repeat sequences (hrs), which typically consist of a few repeated sequences with imperfect palindromes that are interspersed within the genome. The hrs of different baculoviruses are highly variable (Ferrelli et al 2012), and previous studies have suggested that hrs can act as origins of DNA replication and enhancers of gene expression (Guarino et al 1986;Kool et al 1993;Rodems and Friesen 1995;Theilmann and Stewart 1992). A total of 13 hrs were identified in the CyunNPV genome ( Fig.…”

Section: Repeat Regionsmentioning

confidence: 99%

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Zhu

Wang

et al. 2018

Virol. Sin.

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The Cyclophragma undans nucleopolyhedrovirus (CyunNPV), a potential pest control agent, was isolated from Cyclophragma undans (Lepidoptera: Lasiocampidae), an important forest pest. In the present study, we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group I alphabaculoviruses. Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G + C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group I Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to CyunNPV. In addition, the genome contains 13 homologous repeated sequences (hrs). Phylogenetic analysis groups CyunNPV under a distinct branch within clade "a" of Group I in the genus Alphabaculovirus. Unlike other members of Group I, CyunNPV harbors only nine of the 11 genes previously determined to be specific to Group I viruses. Furthermore, the CyunNPV lacks the tyrosine phosphatase gene and the ac30 gene. The CyunNPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pre-transmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings imply that CyunNPV is a distinct species under Group I Alphabaculovirus.

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Section: Repeat Regionsmentioning

confidence: 99%

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Zhu

Wang

et al. 2018

Virol. Sin.

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“…pAcIE1, pIE1HRGalT, pIE1HRST6, and pIE1Hygro are immediate early expression plasmids that encode AcMNPV IE1 (Guarino and Summers, 1987), bovine ␤1,4-galactosyltransferase (␤1,4GalT; Shaper et al, 1986), rat ␣2,6-sialyltransferase (␣2,6SiaT; Weinstein et al, 1987), or hygromycin phosphotransferase (Yates et al, 1985), respectively, under the transcriptional control of the AcMNPV ie1 promoter (Guarino and Summers, 1987) and hr5 enhancer (Guarino et al, 1986). The construction of each of these plasmids has been described previously (Hollister and Jarvis, 2000;Hollister et al, 1998).…”

Section: Plasmid Constructionsmentioning

confidence: 99%

Improved glycosylation of a foreign protein by Tn‐5B1‐4 cells engineered to express mammalian glycosyltransferases

Breitbach

Jarvis

2001

Biotech & Bioengineering

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The major advantages of using the baculovirus-insect cell system for recombinant protein production are its ability to produce large amounts of recombinant proteins and its ability to provide eucaryotic modifications, such as glycosylation. However, the glycans linked to recombinant glycoproteins produced by this system typically differ from those found on native mammalian products. This is an important problem because glycans on mammalian glycoproteins can influence their functions in many different ways. The inability of baculovirus-infected insect cells to produce glycans identical to those found on native mammalian glycoproteins is due, in part, to the absence of functional levels of certain glycosyltransferases in insect cells. Thus, the purpose of this study was to engineer these activities into Tn-5B1-4, an established insect cell line that is widely used as a host for baculovirus-mediated protein production. Expression plasmids were constructed in which cDNAs encoding mammalian beta1,4-galactosyltransferase and alpha2,6-sialyltransferase were placed under the transcriptional control of a baculovirus immediate early promoter. These plasmids were then used to isolate two different transgenic Tn-5B1-4 derivatives and the biological and biochemical properties of these cell lines were examined. The results show that both of the engineered insect cell lines have improved glycoprotein-processing capabilities, relative to the parental cell line.

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“…Each hr contains from two to eight 24-bp imperfect palindromes with naturally occurring EcoRI sites at their core. The hrs function as enhancers of early viral gene expression (11,12). The baculovirus transactivator IE1 binds to DNA sequences within the enhancer regions (9,10).…”

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confidence: 99%

The lef-3 gene of Autographa californica nuclear polyhedrosis virus encodes a single-stranded DNA-binding protein

Hang

Dong

Guarino

1995

J Virol

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The Autographa californica nuclear polyhedrosis virus (AcNPV) replicates in the nuclei of infected cells and encodes several proteins required for viral DNA replication. As a first step in the functional characterization of viral replication proteins, we purified a single-stranded DNA-binding protein (SSB) from AcNPV-infected insect cells. Nuclear extracts were chromatographed on single-stranded DNA agarose columns. An abundant protein with an apparent molecular weight of 43,000 was eluted from the columns at 0.9 to 1.0 M NaCl. This protein was not evident in extracts prepared from control cells, suggesting that the SSB was encoded by the virus. SSB bound to single-stranded DNA in solution, and binding was nonspecific with respect to base sequence, as single-stranded vector DNA competed as efficiently as single-stranded DNA containing the AcNPV origin of DNA replication. Competition binding experiments indicated that SSB showed a preference for single-stranded DNA over double-stranded DNA. To determine whether SSB was encoded by the lef-3 gene of AcNPV, the lef-3 open reading frame was cloned under the control of the bacteriophage T7 promoter. Immunochemical analyses indicated that LEF-3 produced in bacteria or in rabbit reticulocyte lysates specifically reacted with antiserum produced by immunization with purified SSB. Immunoblot analyses of infected cell extracts revealed that SSB/LEF-3 was detected by 4 h postinfection and accumulated through 48 h postinfection.

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Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus

Cited by 163 publications

References 35 publications

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

Improved glycosylation of a foreign protein by Tn‐5B1‐4 cells engineered to express mammalian glycosyltransferases

The lef-3 gene of Autographa californica nuclear polyhedrosis virus encodes a single-stranded DNA-binding protein

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