Complete sequence and genome properties of Chinese wheat mosaic virus, a new furovirus from China.

Diao, Aipo; Chen, Jianping; Yao, Rong; Zheng, Tao; Yu, Shanquan; Antoniw, J. F.; Adams, M. J.

doi:10.1099/0022-1317-80-5-1141

Cited by 71 publications

(58 citation statements)

References 9 publications

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“…The putative methyltransferase region was most similar to sequences of Soil-borne cereal mosaic virus (Diao et al, 1999a;Koenig et al, 1999), Soil-borne wheat mosaic virus (Shirako & Wilson, 1993;Ratti et al, 2004) and Oat golden stripe virus (Diao et al, 1999a) (unassigned members of Furovirus), Fragaria chiloensis latent virus (Tzanetakis & Martin, 2005) (Bromoviridae: Ilarvirus), Chinese wheat mosaic virus (Yang et al, 2001;Diao et al, 1999b) (unclassified Furovirus) and Pepper mild mottle virus (Velasco et al, 2002; GenBank accession no. BAD90599) (unassigned Tobamovirus), and the expect value ranged from 3.5E-06 to 6E-03.…”

mentioning

confidence: 79%

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

Tuomivirta¹,

Kaitera²,

Hantula³

2009

Journal of General Virology

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A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities Ascospore and mycelial isolates of Gremmeniella abietina type B were found to contain three different dsRNA molecules with approximate lengths of 11, 5 and 3 kb. The 11 kb dsRNA encoded the genome of a putative virus and is named Gremmeniella abietina type B RNA virus XL (GaBRV-XL). GaBRV-XL probably exists in an unencapsulated state. We identified two distinct dsRNAs (10 374 and 10 375 bp) of GaBRV-XL, both of which coded for the same putative polyprotein (3249 amino acids) and contained four regions similar to putative viral methyltransferases, DExH box helicases, viral RNA helicase 1 and RNA-dependent RNA polymerases. While a cysteine-rich region with several CxCC motifs in GaBRV-XL was similar to that of putative endornaviruses, cluster analyses of conserved regions revealed GaBRV-XL to be distinct from a broad range of viral taxa but most closely related to Discula destructiva virus 3. Collectively, these findings suggest that GaBRV-XL represents a novel virus group related to endornaviruses. INTRODUCTIONRecently recognized by the International Committee on Taxonomy of Viruses (ICTVdB Management, 2006), endornaviruses are usually cryptic and non-enveloped plant and fungal dsRNA viruses that spread efficiently through mitotic and meiotic cells but not through grafts (Fukuhara et al., 1995;Moriyama et al., 1999;Wakarchuk & Hamilton, 1990;Pfeiffer, 1998 Fungal viruses of the G. abietina species complex have been studied thoroughly in type A and are known to include putative members of the virus families Narnaviridae, Totiviridae and Partitiviridae, some of which can co-infect a single fungal isolate (Tuomivirta et al., 2002;Tuomivirta & Hantula, 2005).The goals of this study include a survey of dsRNA molecules in G. abietina type B, molecular characterization of the most common dsRNA type and its comparison to representatives of similar viral taxa. METHODSG. abietina type B strains. G. abietina type B strains (see Supplementary Table S1, available in JGV Online) were identified by the random amplified microsatellite (RAMS) technique of Hantula & Müller (1997). Branches from P. sylvestris and Pinus contorta ,2 m in height (,20 years old) with symptoms of scleroderris canker were collected in artificially or naturally regenerated stands between June 1994 and June 1995 in northern Finland. Disease symptoms included death of lateral branches over several internodes, cankers, yellowgreen woody tissues, pycnidia and apothecia. Fungi were isolated either from pycnidia or from infected wood adjacent to apothecia.Nucleic acid isolation and electrophoresis. G. abietina type B isolates were grown at 20 uC on modified orange serum agar covered with a cellophane membrane (Müller et al., 1994). dsRNA isolationThe GenBank/EMBL/DDBJ accession numbers for the complete sequences of the 11 kb dsRNAs of isolates AU58 (GaBRV-XL1) and E46 (GaBRV-XL2) are respectively DQ399289 and DQ399290.Details of ...

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mentioning

confidence: 79%

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

Tuomivirta¹,

Kaitera²,

Hantula³

2009

Journal of General Virology

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“…These viruses are transmitted to the roots of their host plants by Polymyxa graminis Led., a soil-borne plasmodiophorid protist that can preserve its infectivity in soil for 10 years or more (Rao and Brakke 1969;Kendall and Lommel 1988). SBCMV is prevalent in Europe, CWMV in Asia, and SBWMV (the furovirus type member) in North America (Diao et al 1999;Kanyuka et al 2003;Ratti et al 2005;Budge et al 2008). In Italy, SBCMV is widespread in the main wheat growing areas, and especially in the northern and central regions of Italy, where it causes grain yield losses often above 50% on susceptible cultivars of durum wheat (AABB genome) and common wheat This thus suggests that this cultivar and its derivatives carry a major gene or gene-block for SBCMV resistance.…”

Section: Introductionmentioning

confidence: 99%

A major QTL for resistance to soil-borne cereal mosaic virus derived from an old Italian durum wheat cultivar

Russo

Ficco

Marone

et al. 2011

Journal of Plant Interactions

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“…Bars below the aa sequence alignment indicate identified NLSs. +, Amino acid positions of 16K PpK20 PS mutants displaying tolerance (class I and II mutants) to pentapeptide insertions in silencing suppression ability; $, 16K residues highly conserved between CRPs derived from different virus genera (Morozov et al, 1989;Savenkov et al, 1998;Diao et al, 1999;Te et al, 2005). TRV 16K-PpK20 (GenBank accession no.…”

Section: Subcellular Localization Of Trv 16kmentioning

confidence: 99%

“…Thus, it can be concluded that the 16K suppression of gene silencing is organism-specific, depending on the assay applied. It is well-known from several previous studies that CRPs from different plant RNA viruses possess only distant sequence homology relationships (Koonin et al, 1991;Savenkov et al, 1998;Diao et al, 1999;Te et al, 2005). This homology is mainly restricted to a conserved motif (Cys-Gly...Cys-Gly-X-X-His, see the tobravirus CRP alignment in Fig.…”

mentioning

confidence: 99%

“…Tobacco rattle virus (TRV), a member of the genus Tobravirus, is a bipartite positive-sense, single-stranded RNA virus which forms rod-shaped particles (MacFarlane, Supplementary material is available with the online version of this paper. Koonin et al, 1991;Savenkov et al, 1998;Diao et al, 1999;Te et al, 2005). The two clusters of N-terminal cysteine residues of barley stripe mosaic virus (BSMV) CRP cb have been demonstrated to bind zinc experimentally , whereas the TRV 16K cysteine residues have only been suggested to possess zinc-binding properties (MacFarlane et al, 1989).…”

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confidence: 99%

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Functional characterization and subcellular localization of the 16K cysteine-rich suppressor of gene silencing protein of tobacco rattle virus

Ghazala

Waltermann

Pilot

et al. 2008

Journal of General Virology

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The 16 kDa cysteine-rich protein (16K) of tobacco rattle virus (TRV) is known to partially suppress RNA silencing in Drosophila cells. In this study, we show that 16K suppresses RNA silencing in green fluorescent protein (GFP)-transgenic Nicotiana benthamiana plants using an Agrobacterium-mediated transient assay. 16K slightly reduced the accumulation of short interfering RNAs (siRNA) of GFP, suggesting that the protein may interfere with the initiation and/ or maintenance of RNA silencing. Deletion of either the N-or C-terminal part of 16K indicated that the entire 16K open reading frame (ORF) is necessary for its silencing suppression function. Pentapeptide insertion scanning mutagenesis (PSM) revealed that only two short regions of 16K tolerated five extra amino acid insertions without considerable reduction in its silencing suppression function. The tolerant regions coincide with sequence variability between tobravirus cysteine-rich proteins, indicating a strong functional and/or structural conservation of TRV 16K. Confocal laser scanning microscopy of transiently expressed 16K fusions to red fluorescent protein (RFP) revealed a predominant cytoplasmic localization and, in addition, a nuclear localization. In contrast, fusions of RFP with the N-terminal region of 16K localized exclusively to the cytoplasm, whereas fusions between RFP and the C-terminal region of 16K displayed an exclusive nuclear localization. Further analysis of 16K-derived peptide fusions demonstrated that the 16K C-terminal region contained at least two functional bipartite nuclear localization signals which were independently capable of nuclear targeting. INTRODUCTIONRNA silencing in higher plants represents a natural host defence response involving the selective degradation of invading virus RNAs (Voinnet, 2001;Moissiard & Voinnet, 2004). Double-stranded RNAs (dsRNA) (Bass, 2000), formed during the replication of plant RNA viruses, are the key trigger molecules of virus-induced RNA silencing. After processing of dsRNAs into 21-26 nt double-stranded fragments by an RNase III-type Dicer endonuclease (Bernstein et al., 2001), these so-called small interfering RNAs (siRNAs) (Hamilton & Baulcombe, 1999;Elbashir et al., 2001) are integrated into a multicomponent RNase, the RNA-induced silencing complex (RISC), which then mediates specific cleavage of complementary single-stranded RNA (ssRNA) (Hammond et al., 2000). To counteract this resistance mechanism, plant viruses have evolved proteins that suppress RNA silencing at different stages (Moissiard & Voinnet, 2004; Silhavy & Burgyán, 2004;Voinnet, 2005). To date, plant virus silencing suppressors of 23 different virus genera have been identified, displaying high genetic diversity (Li & Ding, 2006). The current knowledge on silencing suppressor proteins and the molecular mechanisms of silencing suppression has been reviewed recently (Moissiard & Voinnet, 2004; Silhavy & Burgyán, 2004;Li & Ding, 2006). Although the molecular basis for suppressor activity has been described only for selected vir...

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Complete sequence and genome properties of Chinese wheat mosaic virus, a new furovirus from China.

Cited by 71 publications

References 9 publications

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

A major QTL for resistance to soil-borne cereal mosaic virus derived from an old Italian durum wheat cultivar

Functional characterization and subcellular localization of the 16K cysteine-rich suppressor of gene silencing protein of tobacco rattle virus

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