Complete Sequence and Organization of pFR260, the &lt;b&gt;&lt;i&gt;Bacillus thuringiensis&lt;/i&gt;&lt;/b&gt; INTA Fr7-4 Plasmid Harboring Insecticidal Genes

Navas, Laura Emilce; Amadío, Ariel Fernando; Ortiz, Maximiliano; Sauka, Diego Herman; Benintende, Graciela Beatriz; Berretta, Marcelo Facundo; Zandomeni, Rubén

doi:10.1159/000451056

Cited by 6 publications

(4 citation statements)

References 63 publications

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“…no. KX258624) megaplasmid harbored by strain INTA Fr7-4 strain [ 21 ]. This megaplasmid, 260,595 bp in size, encodes Cry8Qa2, Cry8Kb3, and Cry8Pa3 proteins, along with two pairs of binary proteins, namely, Vpb1Ea1/Vpa2Ah1 and Vpb1Ea2/VpaAh2.…”

Section: Resultsmentioning

confidence: 99%

“…The genome sequence from strain Bt-UNVM-84 harbors seven insecticidal-like CDSs, showing similarity to well-known insecticidal proteins, including two pairs of binary Vpb1/Vpa2 proteins plus three Cry proteins showing similarity to Cry8 proteins ( Table 1 ). Analysis by reads mapping has shown that the insecticidal CDSs are highly conserved with those already described in plasmid pFR206 [ 21 ]. Strain Bt_UNVM-84 also encodes CDSs showing similarity to the Mpp4Aa1, Mpp46Ab1, and Xpp22Ba1 proteins, with the last one absent in strain INTA Fr7-4, which has been described to exhibit insecticidal activity against A. grandis and D. virgifera virgifera [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)

Sauka,

Peralta,

Pérez

et al. 2023

Toxins

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Bacillus thuringiensis is a Gram-positive bacterium known for its insecticidal proteins effective against various insect pests. However, limited strains and proteins target coleopteran pests like Anthonomous grandis Boheman, causing substantial economic losses in the cotton industry. This study focuses on characterizing a Bacillus sp. strain, isolated from Oncativo (Argentina), which exhibits ovoid to amorphous parasporal crystals and was designated Bt_UNVM-84. Its genome encodes genes for the production of two pairs of binary Vpb1/Vpa2 proteins and three Cry-like proteins showing similarity with different Cry8 proteins. Interestingly, this gene content was found to be conserved in a previously characterized Argentine isolate of B. thuringiensis designated INTA Fr7-4. SDS-PAGE analysis revealed a major band of 130 kDa that is proteolytically processed to an approximately 66-kDa protein fragment by trypsin. Bioassays performed with spore-crystal mixtures demonstrated an interesting insecticidal activity against the cotton boll weevil A. grandis neonate larvae, resulting in 91% mortality. Strain Bt_UNVM-84 is, therefore, an interesting candidate for the efficient biological control of this species, causing significant economic losses in the cotton industry in the Americas.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bacillus thuringiensis Bt_UNVM-84, a Novel Strain Showing Insecticidal Activity against Anthonomus grandis Boheman (Coleoptera: Curculionidae)

Sauka,

Peralta,

Pérez

et al. 2023

Toxins

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“…So the development of NGS technologies or metagenomic studies has provided the best way to understand the host-pathogen system. Through this technology, we can construct genome models of different organisms, which includes strains, their natural populations over time and their evolutionary histories (Navas et al, 2017;Sharma et al, 2021). These complicated interactions can be analyzed and integrated by viewing plant microbiomes as a system.…”

Section: Green Grammentioning

confidence: 99%

Interaction between bacterial endophytes and host plants

et al. 2023

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Endophytic bacteria are mainly present in the plant’s root systems. Endophytic bacteria improve plant health and are sometimes necessary to fight against adverse conditions. There is an increasing trend for the use of bacterial endophytes as bio-fertilizers. However, new challenges are also arising regarding the management of these newly discovered bacterial endophytes. Plant growth-promoting bacterial endophytes exist in a wide host range as part of their microbiome, and are proven to exhibit positive effects on plant growth. Endophytic bacterial communities within plant hosts are dynamic and affected by abiotic/biotic factors such as soil conditions, geographical distribution, climate, plant species, and plant-microbe interaction at a large scale. Therefore, there is a need to evaluate the mechanism of bacterial endophytes’ interaction with plants under field conditions before their application. Bacterial endophytes have both beneficial and harmful impacts on plants but the exact mechanism of interaction is poorly understood. A basic approach to exploit the potential genetic elements involved in an endophytic lifestyle is to compare the genomes of rhizospheric plant growth-promoting bacteria with endophytic bacteria. In this mini-review, we will be focused to characterize the genetic diversity and dynamics of endophyte interaction in different host plants.

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“…B. thuringiensis INTA Fr7-4 is a strain isolated from a soil sample in the province of Misiones, Argentina. We have previously reported the complete sequences of four plasmids from this strain named pFR12, pFR12.5, pFR55, and pFR260, according to their length in kilobase pairs ( 1 , 2 ). We have also characterized the insecticidal genes cry8Kb3 , cry8Pa3, and cry8Qa2 ( 3 , 4 ) present in a pathogenicity island, along with two vip2-vip1 operons in pFR260 ( 2 ).…”

Section: Genome Announcementmentioning

confidence: 99%