Complete Sequence of Bacillus subtilis Plasmid p1414 and Comparison with Seven Other Plasmid Types Found in Russian Soil Isolates of Bacillus subtilis

Thorsted, Peter; Thomas, Christopher M.; Полуэктова, Е. У.; Prozorov, A. A.

doi:10.1006/plas.1999.1393

Cited by 21 publications

(16 citation statements)

References 29 publications

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“…Upstream from oriT is a large ORF, ORF19, which encodes a predicted protein that has similarity to the TraA or Mob proteins encoded by various plasmids of grampositive bacteria, including pIP501 and pMRC01 (10,48). In addition, there is another possible Mob coding region (ORF9) whose product exhibits amino acid similarity to the products of ORFs on rolling circle plasmids pTA1015, pTA1040, and pTA1060 (29) and p1414 of Bacillus subtilis (44). Adjacent to this gene is a possible single strand origin (sso) on the opposite strand whose sequence and position are similar to those of the same B. subtilis plasmids (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…A possible glutathione synthase S6 ribosomal modifying enzyme (ORF3, GshB) was present, and the highest level of similarity was the level of similarity to an enzyme encoded by the recently sequenced genome of the deepsea extremely halotolerant and alkaliphilic species Oceanobacillus iheyensis, a microorganism related to Bacillus (42). In addition, a putative biotin carboxylase (encoded by ORF5) (44). Eleven other possible coding regions had similarity to regions encoding hypothetical proteins with no known functions in various genera and plasmids and are listed in Table 2.…”

Section: Resultsmentioning

confidence: 99%

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Molecular Characterization of Plasmid pBM300 from Bacillus megaterium QM B1551

Kunnimalaiyaan

Vary

2005

Appl Environ Microbiol

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Strain QM B1551 of Bacillus megaterium contains seven compatible plasmids: two small rolling circle plasmids and five theta-replicating plasmids with cross-hybridizing replicons. To expand our understanding of these plasmids, the replicon region (6.7 kb) from pBM300 was cloned, sequenced, and functionally characterized. Sequence analysis showed that the replication protein (RepM300) was highly homologous to two other plasmid Rep proteins of the same strain but to no other known proteins. Furthermore, the location of the replication origin was within the RepM300 coding region, and the origin contained three 12-base direct repeats. Deletion analysis of the replicon confirmed the role of the Rep protein and showed that open reading frame 2 (ORF2) was required for stability. However, the protein encoded by ORF2 is entirely different from the replicon stability proteins encoded by the other two replicons. The entire plasmid was isolated from the plasmid array by integrating a spectinomycin resistance gene and transforming a plasmidless strain, PV361. Complete sequencing showed that pBM300 was 26,300 bp long, had a G؉C content of 35.2%, and contained 20 ORFs, two of which encoded proteins that had no similarity to other proteins in the database. The proteins encoded by the plasmid ORFs had similarity to proteins for mobilization and transfer, an integrase, a rifampin resistance protein, a cell wall hydrolase, glutathione synthase, and a biotin carboxylase. The similarities were to several gram-positive genera and a few gram-negative genera and archaea. oriT and ssoT-like regions were detected near two mob genes. These results suggest that pBM300 is a mobilizable hybrid plasmid that confers increased metabolic and germination ability on its host. Its replicon also helps define a new plasmid family.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Plasmid pBM300 from Bacillus megaterium QM B1551

Kunnimalaiyaan

Vary

2005

Appl Environ Microbiol

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“…plasmid-containing strains are shown in Table 1. Oligonucleotides BACREPF (5P-GGT TAT GYC CKA TGT GTG C-3P) and BACREPR2 (5P-TCC GTA TCY TTM ACY GGA TA-3P) to amplify partial rep gene regions were designed to conserved regions in the rep genes from the Bacillus RCR plasmids: pTA1015, pTA1040, pTA1060 [3], pFTB14 [10], pBAA1 [11], pBS2 [12], p1414 [13], pPL10 (AF036712), pSH1452 [14] and pUH1 [15]. Primers BACMOBF (5P-GTC AAA CGG AAG GTC CGA GC-3P) and BACMOBR (5P-TCA TGT TCA ATA GTC GGC AT-3P) were designed to conserved regions in the mob genes of pTA1015, pTA1060 [3], p1414 [13] and pUH1 [15].…”

Section: Bacterial Strains Plasmids and Oligonucleotidesmentioning

confidence: 99%

Diversity and linkage of replication and mobilisation genes in Bacillus rolling circle-replicating plasmids from diverse geographical origins

Mason

2002

FEMS Microbiology Ecology

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The prevalence, diversity and linkage of pTA-related plasmid rep and mob genes in 18 plasmids from three closely related species of Bacillus from diverse geographical locations have been investigated using PCR. pTA-related rep and mob sequences were both amplified from 13 of the 18 plasmids. For one plasmid, pBM9, only a pTA-related mob gene was amplified, whilst only a pTA-related rep gene was amplified from the Bacillus licheniformis plasmid pBL2. No products were amplified for either gene from two other B. licheniformis plasmids or from a larger 16-kb Bacillus subtilis plasmid, pBS6. Whilst simple gene detection suggests close linkage of both pTA-related rep and mob genes, on most of these plasmids, sequence analysis of amplified genes revealed more complex linkage relationships varying with geographical origin, plasmid size and bacterial host.

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“…ORF6 seems to be a conserved gene product encoded by plasmids from many gram-positive bacteria and is present on at least five other QM B1551 plasmids. It is similar to possible DNA binding proteins encoded by other plasmids, including ORF2C by B. subtilis plasmids pTA1060 and pTA1040 (33), Ptr by p1414 (54), and ORF 68 by B. anthracis virulence plasmid pXO2 (36).…”

Section: Construction Of An Integrative-replicative Vectormentioning

confidence: 70%

Sequencing and Characterization of pBM400 from Bacillus megaterium QM B1551

Scholle

White

Kunnimalaiyaan

et al. 2003

Appl Environ Microbiol

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Bacillus megaterium QM B1551 plasmid pBM400, one of seven indigenous plasmids, has been labeled with a selectable marker, isolated, completely sequenced, and partially characterized. A sequence of 53,903 bp was generated, revealing a total of 50 predicted open reading frames (ORFs); 33 were carried on one strand and 17 were carried on the other. These ORFs comprised 57% of the pBM400 sequence. Besides the replicon region and a complete rRNA operon that have previously been described, several interesting genes were found, including genes for predicted proteins for cell division (FtsZ and FtsK), DNA-RNA interaction (FtsK, Int/Rec, and reverse transcriptase), germination (CwlJ), styrene degradation (StyA), and heavy metal resistance (Cu-Cd export and ATPase). Three of the ORF products had high similarities to proteins from the Bacillus anthracis virulence plasmid pXO1. An insertion element with similarity to the IS256 family and several hypothetical proteins similar to those from the chromosomes of other Bacillus and Lactococcus species were present. This study provides a basis for isolation and sequencing of other high-molecular-weight plasmids from QM B1551 and for understanding the role of megaplasmids in gram-positive bacteria. The genes carried by pBM400 suggest a possible role of this plasmid in the survival of B. megaterium in hostile environments with heavy metals or styrene and also suggest that there has been an exchange of genes within the gram-positive bacteria, including pathogens.

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Complete Sequence of Bacillus subtilis Plasmid p1414 and Comparison with Seven Other Plasmid Types Found in Russian Soil Isolates of Bacillus subtilis

Cited by 21 publications

References 29 publications

Molecular Characterization of Plasmid pBM300 from Bacillus megaterium QM B1551

Molecular Characterization of Plasmid pBM300 from Bacillus megaterium QM B1551

Diversity and linkage of replication and mobilisation genes in Bacillus rolling circle-replicating plasmids from diverse geographical origins

Sequencing and Characterization of pBM400 from Bacillus megaterium QM B1551

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