Complete sequence of Litopenaeus vannamei (Crustacea: Decapoda) vitellogenin cDNA and its expression in endocrinologically induced sub-adult females

Raviv, S.; Parnes, Shmuel; Segall, Carmen; Davis, Claytus; Sagi, Amir

doi:10.1016/j.ygcen.2005.06.009

Cited by 98 publications

(55 citation statements)

References 47 publications

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“…In L. vannamei, molt-coupled spermatophore disappearance is not merely the result of the reproductive system being 'switched off', like that of seasonal organisms, but the active degradation of old spermatophores before new ones occupy the ampoules. Based on our recent study of moltrelated cyclic vitellogenic activity and Vg gene expression in L. vannamei females (Raviv et al, 2006) and the current study on molt-related cyclic spermatophore degradation in L. vannamei males, it is clear that in these organisms no phenomenon can be separated from the molt cycle and examined in isolation. Because the reproductive organs of males and females develop from essentially the same embryonic tissue, one cannot escape the similarity in the cyclic nature of these systems in L. vannamei.…”

Section: Discussionmentioning

confidence: 91%

“…In penaeids, resources are more or less simultaneously utilized for weight gain and reproduction throughout the molt cycle (Adiyodi, 1985). Penaeid females may spawn several times during a single molt cycle, but if they do not spawn, the oocytes are resorbed before molting takes place (Emmerson, 1980;Emmerson, 1983;Quinitio et al, 1993;Raviv et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Males also have their time of the month! Cyclic disposal of old spermatophores, timed by the molt cycle, in a marine shrimp

Parnes

Raviv

Shechter

et al. 2006

Journal of Experimental Biology

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SUMMARY That sexually mature females go through hormonally regulated reproductive cycles is a well-established phenomenon in sexually reproducing organisms. Males, on the other hand, are commonly regarded as being continuously ready to mate. `Programmed sperm degradation' on a periodic basis or an innate sperm`expiry date' have never been shown. This manuscript describes a newly discovered molt-dependent mechanism by which old sperm is periodically removed from the reproductive system of male Litopenaeus vannamei shrimp. Firstly, it is shown that the spermatophores of males held in captivity become progressively melanized, a process that eventually renders them impotent. Then, by using melanin specks as a color marker, it is demonstrated that this phenomenon can be delayed and even reversed as long as the males remain sexually active. Lastly and most importantly, it is shown that male shrimp go through reproductive cycles that are strictly associated with their molt cycles, which, in turn, are hormonally regulated. Intact intermolt spermatophores disappeared about 12 h premolt, and a new pair of spermatophores appeared in the ampoules the day after the males had molted. This phenomenon was observed in an almost constant portion of males, both those in an all-male population and those in mixed male/female populations,even during the times that the females of those populations were not vitellogenically active. To the best of our knowledge, this is the first report of males of any animal species exhibiting endogenous reproductive cycles, as do females, and of the finding that spermatozoa have a predetermined expiry date, a feature that may possibly contribute to male fitness.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Males also have their time of the month! Cyclic disposal of old spermatophores, timed by the molt cycle, in a marine shrimp

Parnes

Raviv

Shechter

et al. 2006

Journal of Experimental Biology

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“…Additional sequences of LLTPs from arachnids may help define the context of the divergence of M-177. Importantly, the Vtg of decapodan Crustacea is not directly related to the Vtg genes of other taxons, as assumed by others (52)(53)(54)(55)(56)(57)(58)(59)(60), but is an apoB-like LLTP. This observation explains previous phylogenetic analyses on Vtgs only (57,62,69,70), in which decapodan Vtg failed to group with other invertebrate Vtgs.…”

Section: Three Families Of Lltpsmentioning

confidence: 87%

“…In another class of arthropods, the decapodan Crustacea (e.g., lobster, crayfish, crab, shrimp), two distinct LLTPs have been identified, a CP (7,8) and a yolk protein named Vtg (52)(53)(54)(55)(56)(57)(58)(59)(60). Although both LLTPs have been identified only in distinct species, the relatively close relationship among these species as well as the divergence of the identified proteins strongly suggest that the subset of LLTPs present in decapodan Crustacea con-sists at least of one CP and one Vtg.…”

Section: Lltp Family Membersmentioning

confidence: 99%

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Molecular diversity and evolution of the large lipid transfer protein superfamily

Smolenaars

Madsen

Rodenburg

et al. 2007

Journal of Lipid Research

158

160

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Circulatory lipid transport in animals is mediated to a substantial extent by members of the large lipid transfer (LLT) protein (LLTP) superfamily. These proteins, including apolipoprotein B (apoB), bind lipids and constitute the structural basis for the assembly of lipoproteins. The current analyses of sequence data indicate that LLTPs are unique to animals and that these lipid binding proteins evolved in the earliest multicellular animals. In addition, two novel LLTPs were recognized in insects. Structural and phylogenetic analyses reveal three major families of LLTPs: the apoB-like LLTPs, the vitellogenin-like LLTPs, and the microsomal triglyceride transfer protein (MTP)-like LLTPs, or MTPs. The latter are ubiquitous, whereas the two other families are distributed differentially between animal groups. Besides similarities, remarkable variations are also found among LLTPs in their major lipid-binding sites (i.e., the LLT module as well as the predicted clusters of amphipathic secondary structure): variations such as protein modification and number, size, or occurrence of the clusters. Strikingly, comparative research has also highlighted a multitude of functions for LLTPs in addition to circulatory lipid transport. The integration of LLTP structure, function, and evolution reveals multiple adaptations, which have come about in part upon neofunctionalization of duplicated genes. Moreover, the change, exchange, and expansion of functions illustrate the opportune application of lipid-binding proteins in nature.Accordingly, comparative research exposes the structural and functional adaptations in animal lipid carriers and brings up novel possibilities for the manipulation of lipid transport.

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Identification of Receptor‐Interacting Regions of Vitellogenin within Evolutionarily Conserved β‐Sheet Structures by Using a Peptide Array

et al. 2013

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Vitellogenesis, a key process in oviparous animals, is characterized by enhanced synthesis of the lipoprotein vitellogenin, which serves as the major yolk-protein precursor. In most oviparous animals, and specifically in crustaceans, vitellogenin is mainly synthesized in the hepatopancreas, secreted to the hemolymph, and taken up into the ovary by receptor-mediated endocytosis. In the present study, localization of the vitellogenin receptor and its interaction with vitellogenin were investigated in the freshwater prawn Macrobrachium rosenbergii. The receptor was immuno-histochemically localized to the cell periphery and around yolk vesicles. A receptor blot assay revealed that the vitellogenin receptor interacts with most known vitellogenin subunits, the most prominent being the 79 kDa subunit. The receptor was, moreover, able to interact with trypsin-digested vitellogenin peptides. By combining a novel peptide-array approach with tandem mass spectrometry, eleven vitellogenin-derived peptides that interacted with the receptor were identified. A 3D model of vitellogenin indicated that four of the identified peptides are N-terminally localized. One of the peptides is homologous to the receptor-recognized site of vertebrate vitellogenin, and assumes a conserved β-sheet structure. These findings suggest that this specific β-sheet region in the vitellogenin N-terminal lipoprotein domain is the receptor-interacting site, with the rest of the protein serving to enhance affinity for the receptor. The conservation of the receptor recognition site in invertebrate and vertebrate vitellogenin might have vast implications for oviparous species reproduction, development, immunity, and pest management.

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Complete sequence of Litopenaeus vannamei (Crustacea: Decapoda) vitellogenin cDNA and its expression in endocrinologically induced sub-adult females

Cited by 98 publications

References 47 publications

Males also have their time of the month! Cyclic disposal of old spermatophores, timed by the molt cycle, in a marine shrimp

Males also have their time of the month! Cyclic disposal of old spermatophores, timed by the molt cycle, in a marine shrimp

Molecular diversity and evolution of the large lipid transfer protein superfamily

Identification of Receptor‐Interacting Regions of Vitellogenin within Evolutionarily Conserved β‐Sheet Structures by Using a Peptide Array

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