Complete sequence of the blaNDM-1-carrying plasmid pNDM-AB from Acinetobacter baumannii of food animal origin

Zhang, Wanjiang; Lu, Zexi; Schwarz, Štefan; Zhang, Rong-Min; Wang, Xiumei; Si, Wei; Yu, Shenye; Chen, Liping; Liu, Siguo

doi:10.1093/jac/dkt066

Cited by 103 publications

(85 citation statements)

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“…Such strains have harbored extensive antibiotic resistance repertoires. Furthermore, A. baumannii strains harboring extensive antibiotic resistance have contaminated commercial food, including meat, vegetables, and various types of livestock, suggesting multiple environmental routes of transmission into human populations (3,(17)(18)(19). However, non-baumannii Acinetobacter spp.…”

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Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges

et al. 2017

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SUMMARY Acinetobacter is a complex genus, and historically, there has been confusion about the existence of multiple species. The species commonly cause nosocomial infections, predominantly aspiration pneumonia and catheter-associated bacteremia, but can also cause soft tissue and urinary tract infections. Community-acquired infections by Acinetobacter spp. are increasingly reported. Transmission of Acinetobacter and subsequent disease is facilitated by the organism's environmental tenacity, resistance to desiccation, and evasion of host immunity. The virulence properties demonstrated by Acinetobacter spp. primarily stem from evasion of rapid clearance by the innate immune system, effectively enabling high bacterial density that triggers lipopolysaccharide (LPS)–Toll-like receptor 4 (TLR4)-mediated sepsis. Capsular polysaccharide is a critical virulence factor that enables immune evasion, while LPS triggers septic shock. However, the primary driver of clinical outcome is antibiotic resistance. Administration of initially effective therapy is key to improving survival, reducing 30-day mortality threefold. Regrettably, due to the high frequency of this organism having an extreme drug resistance (XDR) phenotype, early initiation of effective therapy is a major clinical challenge. Given its high rate of antibiotic resistance and abysmal outcomes (up to 70% mortality rate from infections caused by XDR strains in some case series), new preventative and therapeutic options for Acinetobacter spp. are desperately needed.

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Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges

et al. 2017

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“…NDM-1 has also been reported in Acinetobacter spp. from food animals (chicken and pig farms) in China (14,15). However, knowledge about carbapenemase-producing Acinetobacter spp.…”

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“…Comparison of human and animal carbapenem-resistant Acinetobacter isolates is important to enhance the knowledge of the potential routes of transfer of these bacteria and resistance genes in different ecosystems. Few studies have been published describing the dissemination of carbapenem-resistant Acinetobacter isolates in food animals and wild animals (11)(12)(13)(14)(15). All these points and the clonality between a panel of clinical strains showed the possible exchange place between the A. baumannii populations in infected humans and water.…”

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First Description of Two Sequence Type 2 Acinetobacter baumannii Isolates Carrying OXA-23 Carbapenemase in Pagellus acarne Fished from the Mediterranean Sea near Bejaia, Algeria

Brahmi

Touati

Cadière

et al. 2016

Antimicrob Agents Chemother

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e To determine the occurrence of carbapenem-resistant Acinetobacter baumannii in fish fished from the Mediterranean Sea near the Bejaia coast (Algeria), we studied 300 gills and gut samples that had been randomly and prospectively collected during 1 year. After screening on selective agar media, using PCR arrays and whole-genome sequencing, we identified for the first time two OXA-23-producing A. baumannii strains belonging to the widespread sequence type 2 (ST2)/international clone II and harboring aminoglycoside-modifying enzymes [aac(6=)-Ib and aac(3=)-I genes]. Acinetobacter baumannii is an opportunistic aerobic nonfermentative Gram-negative rod found ubiquitously in the environment (1). This bacterium has emerged as an important cause of nosocomial infections, most notably ventilator-associated pneumonia and bacteremia associated with high mortality, urinary tract infections, and endocarditis (2). Moreover, it is highly capable of developing resistance to antimicrobial agents (1). Over the last 10 years, an increase in carbapenem-resistant A. baumannii strains has been observed worldwide; in particular, we noted a high prevalence in different countries in the south of Europe (3). The most common mechanism of carbapenem resistance in Acinetobacter species is the production of acquired carbapenem-hydrolyzing OXA-type class D ␤-lactamases (4). They are represented worldwide by six gene clusters: intrinsic chromosomal OXA-51-like, of which there are over 70 variants, and the acquired OXA-23-like, OXA-24/40-like, OXA-58-like, OXA-143-like, and OXA-235-like ␤-lactamases (5-7). Worldwide dissemination of OXA-23-producing A. baumannii is now well established and, notably, has been observed among Algerian hospitals in recent years (8-10). While A. baumannii is isolated from patients and hospital environmental sources during outbreaks, the reservoir outside the hospital is not well delineated. Several investigators have suspected that the survival of A. baumannii in the environment (in particular in water) could contribute to the transmission of the organism during outbreaks (3). Moreover, recent reports have also described the presence of carbapenemresistant Acinetobacter spp. from animals. The OXA-23 carbapenemase has been found in Acinetobacter spp. from cattle, horses, and cats (11-13). NDM-1 has also been reported in Acinetobacter spp. from food animals (chicken and pig farms) in China (14, 15). However, knowledge about carbapenemase-producing Acinetobacter spp. of animal origin remains very limited, making it difficult to assess its impact on public health.Between 1 March 2012 and 28 February 2013, we randomly and prospectively screened a total of 300 samples from different fish fished in the Mediterranean Sea (2 km from the Bejaia coast, Algeria). Sampling was carried out from Sardina pilchardus (n ϭ 62), Engraulis encrasicolus (n ϭ 38), Trachurus trachurus (n ϭ 45), Sarpa salpa (n ϭ 60), Pagellus acarne (n ϭ 55), and Boops boops (n ϭ 40). The gills and gut of each fish sample were collected by opening the gut...

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“…It has been proposed that the dissemination of the bla NDM-1 gene among Acinetobacter strains is mediated by a composite transposon designated Tn125, with two ISAba125 copies bracketing the resistance gene module (6). Although in Acinetobacter the bla NDM-1 has most frequently been found chromosomally located, some reports have described this gene located on plasmids (7,8).…”

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Characterization of Tn 3000 , a Transposon Responsible for bla _NDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

Campos

Silva

Santos³

et al. 2015

Antimicrob Agents Chemother

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kIn Enterobacteriaceae, the bla NDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the bla NDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFII K and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the bla NDM-1 gene may be transmitted by Tn3000 in different parts of the world.

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Complete sequence of the blaNDM-1-carrying plasmid pNDM-AB from Acinetobacter baumannii of food animal origin

Cited by 103 publications

References 10 publications

Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges

Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges

First Description of Two Sequence Type 2 Acinetobacter baumannii Isolates Carrying OXA-23 Carbapenemase in Pagellus acarne Fished from the Mediterranean Sea near Bejaia, Algeria

Characterization of Tn 3000 , a Transposon Responsible for bla _NDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

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Complete sequence of the blaNDM-1-carrying plasmid pNDM-AB from Acinetobacter baumannii of food animal origin

Cited by 103 publications

References 10 publications

Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges

Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges

First Description of Two Sequence Type 2 Acinetobacter baumannii Isolates Carrying OXA-23 Carbapenemase in Pagellus acarne Fished from the Mediterranean Sea near Bejaia, Algeria

Characterization of Tn 3000 , a Transposon Responsible for bla NDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

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Characterization of Tn 3000 , a Transposon Responsible for bla _NDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India