Complete sequence of the floR-carrying multiresistance plasmid pAB5S9 from freshwater Aeromonas bestiarum

Gordon, Laurence; Cloeckaert, Axel; Doublet, Benoît; Schwarz, Štefan; Bouju-Albert, Agnès; Ganière, Jean‐Pierre; Bris, Hervé Le; Flèche‐Matéos, Anne Le; Giraud, Étienne

doi:10.1093/jac/dkn166

Cited by 68 publications

(48 citation statements)

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“…The pAB5S9 plasmid was first discovered in France (19). Our study identified, for the first time, a plasmid in the pAB5S9 family isolated in North America from a different Aeromonas species.…”

Section: Resultsmentioning

confidence: 70%

“…1, part A) had a complete putatively functional transposase and a truncated transposase (delta-tnp). These transposases have been reported to be part of an ISCR2 element in A. bestiarum (19). The other region (Fig.…”

Section: Resultsmentioning

confidence: 87%

“…salmonicida. Further analyses revealed a high level of homology with pAB5S9, a previously described plasmid from Aeromonas bestiarum (19), which carries multiple antibiotic resistance genes. pAB5S9b, the 2009-144K3 variant, exhibited a highly conserved gene distribution and gene order with its homolog except for a transposition of two regions (Fig.…”

Section: Resultsmentioning

confidence: 93%

“…The region is cut in half in pAB5S9 by the tetR and tet(Y) genes (19). Interestingly, the SXT resistance region in pAB5S9b in A. salmonicida subsp.…”

Section: Resultsmentioning

confidence: 99%

“…The large unmapped contigs were investigated by conducting BLASTx and BLASTn searches of the NCBI databases nr/nt. For the 2009-144K3 isolate, two contigs exhibited high degrees of identity with the pAB5S9 plasmid (19), while one contig exhibited a high degree of identity with the pRAS3 plasmid (8). The two pAB5S9-like contigs were assembled using Consed version 35 (20), and the junctions were verified by PCR amplifications.…”

Section: Methodsmentioning

confidence: 99%

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Detection of Variants of the pRAS3, pAB5S9, and pSN254 Plasmids in Aeromonas salmonicida subsp. salmonicida: Multidrug Resistance, Interspecies Exchanges, and Plasmid Reshaping

Vincent

Trudel

Paquet

et al. 2014

Antimicrob Agents Chemother

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The ubiquitous water-borne Gram-negative bacterium Aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a worldwide disease in fish farms. Plasmids carrying antibiotic resistance genes have already been described for this bacterium. The aim of the present study was to identify and characterize additional multidrug resistance plasmids in A. salmonicida subsp. salmonicida. We sequenced the plasmids present in two multiple antibiotic-resistant isolates using highthroughput technologies. We also investigated 19 other isolates with various multidrug resistance profiles by genotyping PCR and assessed their resistance to tetracycline. We identified variants of the pAB5S9 and pSN254 plasmids that carry several antibiotic resistance genes and that have been previously reported in bacteria other than A. salmonicida subsp. salmonicida, which suggests a high level of interspecies exchange. Genotyping analyses and the antibiotic resistance profiles of the 19 other isolates support the idea that multiple versions of pAB5S9 and pSN254 exist in A. salmonicida subsp. salmonicida. We also identified variants of the pRAS3 plasmid. The present study revealed that A. salmonicida subsp. salmonicida harbors a wide variety of plasmids, which suggests that this ubiquitous bacterium may contribute to the spread of antibiotic resistance genes in the environment. The Gram-negative bacterium Aeromonas salmonicida subsp. salmonicida is an opportunistic fish pathogen (1). It is the etiological agent of furunculosis, a disease that especially affects salmonids in fish farms (2). While antibiotics are commonly used to treat A. salmonicida subsp. salmonicida infections, multidrug-resistant isolates have been frequently detected (3-5), preventing the effective treatment of furunculosis.Many fully characterized plasmids from A. salmonicida subsp. salmonicida have provided antibiotic resistance to this species (2). All the known plasmids in A. salmonicida subsp. salmonicida harboring antibiotic resistance genes include at least a tetracycline resistance gene. The vast majority of the plasmids bearing antibiotic resistance genes confer multiple types of resistance to A. salmonicida subsp. salmonicida, including the large (167-kb) plasmid pAsa4, which provides resistance against chloramphenicol, spectinomycin, streptomycin, sulfonamides, tetracycline, mercury, and quaternary ammonium compounds (6). A plasmid bearing multiple resistance genes that is similar to the large pSN254 plasmid in Salmonella enterica (7) has been partially described in A. salmonicida subsp. salmonicida (3). This pSN254-like plasmid can be transferred via conjugation from A. salmonicida subsp. salmonicida to multiple receivers, including Escherichia coli, Edwardsiella tarda, and Aeromonas hydrophila (3).Plasmid variants appear to be relatively frequent in A. salmonicida subsp. salmonicida. The best example is the pRAS3 plasmid. To date, two variants of this plasmid (pRAS3.1 and pRAS3.2) have been described (8). The differences between them are very s...

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Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 93%

“…The region is cut in half in pAB5S9 by the tetR and tet(Y) genes (19). Interestingly, the SXT resistance region in pAB5S9b in A. salmonicida subsp.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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