Complete sequence of the IncPβ plasmid R751: implications for evolution and organisation of the IncP backbone

Thorsted, Peter; Macartney, Donia P.; Akhtar, Parveen; Haines, Anthony S.; Ali, Nasima; Davidson, Philip R.; Stafford, Theresa; Pocklington, Michael J.; Pansegrau, Werner; Wilkins, Brian M.; Lanka, Erich; Thomas, Christopher M.

doi:10.1006/jmbi.1998.2060

Cited by 205 publications

(249 citation statements)

References 94 publications

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“…Previous approaches to examine the role of horizontal gene transfer in microbial evolution have involved the use of a comparative approach to reconstruct historical gene transfer events (for example, Boucher et al, 2003;Kunin et al, 2005;Comas et al, 2006;Doolittle and Bapteste, 2007), the use of in vivo or in vitro gene transfer experiments where gene transfer rates are estimated under controlled conditions (for example, Dahlberg et al, 1998;van Elsas and Bailey, 2002) or molecular investigations of cellular regulation and machinery involved in the process (for example, Thorsted et al, 1998;Lambertsen et al, 2004). While these methods have merit for revealing evolutionary relationships among organisms and the mechanisms that lead to transfer, they rarely reflect in situ gene transfer potential in microbial assemblages.…”

Section: Introductionmentioning

confidence: 99%

Influence of industrial contamination on mobile genetic elements: class 1 integron abundance and gene cassette structure in aquatic bacterial communities

et al. 2008

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The acquisition of new genetic material via horizontal gene transfer allows bacteria to rapidly evolve. One key to estimating the contribution of horizontal gene transfer to bacterial evolution is to quantify the abundance of mobile genetic elements (MGEs) in bacterial communities under varying degrees of selective pressure. We quantified class 1 integrase (intI1) gene abundance in total community DNA extracted from contaminated and reference riverine and estuarine microhabitats, and in metal-or antibiotic-amended freshwater microcosms. The intI1 gene was more abundant in all contaminant-exposed communities indicating that relative gene transfer potential is higher in these communities. A second key to assessing the contributions of MGEs to bacterial evolution is to examine the structure and function of the MGE-associated gene pool. We determined that the gene cassette pool is a novel and diverse resource available for bacterial acquisition, but that contamination has no discernible effect on cassette richness. Gene cassette profiles were more similar within sites than among sites, yet bacterial community profiles were not, suggesting that selective pressures can shape the structure of the gene cassette pool. Of the 46 sequenced gene cassette products, 37 were novel sequences, while the 9 gene cassettes with similarity to database sequences were primarily to hypothetical proteins. That class 1 integrons are ubiquitous and abundant in environmental bacterial communities indicates that this group of MGEs can play a substantial role in the acquisition of a diverse array of gene cassettes beyond their demonstrated impact in mediating multidrug resistance in clinical bacteria.

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Section: Introductionmentioning

confidence: 99%

Influence of industrial contamination on mobile genetic elements: class 1 integron abundance and gene cassette structure in aquatic bacterial communities

et al. 2008

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“…From the 1970s, many new members of the IncP-1 group have been discovered in bacteria from aquatic and soil environments (pB3, Heuer et al, 2004; pADP-1, Martinez et al, 2001; pB10, Schlüter et al, 2003; pB8, Schlüter et al, 2005; pUO1, Sota et al, 2003; pB4, Tauch et al, 2003; pTB11, Tennstedt et al, 2005; pJP4, Trefault et al, 2004; pEST4011, Vedler et al, 2004). The complete genome sequences of these newly identified IncP-1 plasmids have been determined, and the sequence data revealed that these plasmids have IncP-1-specific backbone modules for their replication, stable inheritance and conjugative transfer which show high similarity to the corresponding modules of two wellcharacterized IncP-1 plasmids, the IncP-1a subgroup plasmid RP4 (Pansegrau et al, 1994) and the IncP-1b subgroup plasmid R751 (Thorsted et al, 1998). Their genomes have various mobile genetic elements (transposons and their remnants) that carry genes encoding antibiotic resistance or degradation of xenobiotic compounds.…”

Section: Introductionmentioning

confidence: 99%

Plasmid pBP136 from Bordetella pertussis represents an ancestral form of IncP-1β plasmids without accessory mobile elements

et al. 2006

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The complete 41 268 bp nucleotide sequence of the IncP-1b plasmid pBP136 from the human pathogen Bordetella pertussis, the primary aetiological agent of whooping cough, was determined and analysed. This plasmid carried a total of 46 ORFs: 44 ORFs corresponding to the genes in the conserved IncP-1b backbone, and 2 ORFs similar to the XF1596 and XF1597 genes with unknown function of the plant pathogen Xylella fastidiosa. Interestingly, pBP136 had no accessory genes carrying genetic traits such as antibiotic or mercury resistance and/or xenobiotic degradation. Moreover, pBP136 had only two of the kle genes (kleAE) that have been reported to be important for the stability of IncP-1 plasmid in Pseudomonas aeruginosa. Phylogenetic analysis of the Kle proteins revealed that the KleA and KleE of pBP136 were phylogenetically distant from those of the present IncP-1 plasmids. In contrast, IncC1 and KorC, encoded upstream and downstream of the kle genes respectively, and the replication-initiation protein, TrfA, were closely related to those of the IncP-1b 'R751 group'. These results suggest that (i) pBP136 without any apparent accessory genes diverged early from an ancestor of the present IncP-1b plasmids, especially those of the R751 group, and (ii) the kle genes might be incorporated independently into the backbone region of the IncP-1 plasmids for their stable maintenance in various host cells. INTRODUCTIONThe bacterial incompatibility group P-1 (IncP-1) plasmids can replicate and be stably maintained in almost all Gramnegative bacteria, and these plasmids are very promiscuous as either antibiotic-resistance or xenobiotic-degradative plasmids (Adamczyk & Jagura-Burdzy, 2003;Dennis, 2005;Thomas, 2000;Thomas & Smith, 1987). From the 1970s, many new members of the IncP-1 group have been discovered in bacteria from aquatic and soil environments (pB3, Heuer et al., 2004; pADP-1, Martinez et al., 2001; pB10, Schlüter et al., 2003; pB8, Schlüter et al., 2005; pUO1, Sota et al., 2003; pB4, Tauch et al., 2003; pTB11, Tennstedt et al., 2005; pJP4, Trefault et al., 2004; pEST4011, Vedler et al., 2004). The complete genome sequences of these newly identified IncP-1 plasmids have been determined, and the sequence data revealed that these plasmids have IncP-1-specific backbone modules for their replication, stable inheritance and conjugative transfer which show high similarity to the corresponding modules of two wellcharacterized IncP-1 plasmids, the IncP-1a subgroup plasmid RP4 (Pansegrau et al., 1994) and the IncP-1b subgroup plasmid R751 (Thorsted et al., 1998). Their genomes have various mobile genetic elements (transposons and their remnants) that carry genes encoding antibiotic resistance or degradation of xenobiotic compounds. Most of these accessory genes are inserted in one or both of two specific regions of the backbone, e.g. the IncP-1b plasmid pUO1 contains two haloacetate-catabolic transposons in the oriV-trfA region and two mercury-resistance transposons in the trb-tra region (Sota et al., 2003). In general, IncP-...

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“…Most bacteria are capable of becoming hosts to a large array of different plasmids commonly found in other strains, other species or even other genera. Plasmids thus represent a large genetic resource for diversity and adaptation of bacteria (Thorsted et al, 1998).…”

Section: Plasmidsmentioning

confidence: 99%

Detection of Conjugative Plasmid Encoded Ampicillin and Tetracycline Resistant in Klebsiella pneumonia

Ozdemir¹,

Hassan²,

Kala³

2013

IJB

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Klebsiella pneumonia were isolated and identified on the basis of morphology, growth, and biochemical characteristics. All the isolates offered different resistance patterns against antibiotics including Ampicillin, Cefotaxime, Erythromycin, tetracycline and Chloramphenicol. Transformation and conjugation techniques were used for plasmid transfer studies. The conjugation experiment showed that ≈ 51kbp conjugative plasmid conferring resistance to tetracycline and ampicillins were successfully transferred to the recipient cells E. coli MM294. The rest of the plasmid borne markers was non-conjugative/nontransferable. Conjugative plasmids carry a tremendous potential to disseminate resistance markers to distant recipient cells. The protocol is reliable enough to be used for large-scale visualization of native plasmids and we have used it to visualize and isolate DNA from hundreds of multidrug resistance plasmids.

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Complete sequence of the IncPβ plasmid R751: implications for evolution and organisation of the IncP backbone

Cited by 205 publications

References 94 publications

Influence of industrial contamination on mobile genetic elements: class 1 integron abundance and gene cassette structure in aquatic bacterial communities

Influence of industrial contamination on mobile genetic elements: class 1 integron abundance and gene cassette structure in aquatic bacterial communities

Plasmid pBP136 from Bordetella pertussis represents an ancestral form of IncP-1β plasmids without accessory mobile elements

Detection of Conjugative Plasmid Encoded Ampicillin and Tetracycline Resistant in Klebsiella pneumonia

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