Complete sequences of a novel blaNDM-1-harbouring plasmid from Providencia rettgeri and an FII-type plasmid from Klebsiella pneumoniae identified in Canada

Mataseje, Laura; Lefebvre, Brigitte; Roscoe, Diane; Boyd, David A.; Bryce, Elizabeth; Conly, John; Deheer, J.; Embil, John M.; Embreé, Joanne; Evans, Gregory L.; Forgie, Shaun A.; Frenette, Charles; Lemieux, Claude; Golding, George R.; Gravel, Denise; Henderson, Elizabeth; Hutchinson, J.; John, Michael; Johnston, Leland H.; Katz, Kevin; Kibsey, Pamela; Kuhn, Michael; Langley, Joanne M.; Lesaux, N.; Loeb, Marilyn R.; Matlow, Anne; McGeer, Allison; Miller, Mark A.; Mitchell, Robin E.; Moore, Deanna; Mounchili, Aboubakar; Mulvey, Michael R.; Pelude, Linda; Roth, Virginia; Simor, Andrew E.; Suh, Kathryn N.; Taylor, Geoffrey; Thomas, Evangelos; Turgeon, Nathalie; Vearncombe, Mary; Vayalumkal, Joseph; Weiss, Karl Heinz; Wong, A. Y.

doi:10.1093/jac/dkt445

Cited by 42 publications

(36 citation statements)

References 19 publications

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“…2). The mobile element harboring the bla NDM-1 gene in pHNEC55 and pHNEC46 contained a structure identical to the ones found in an Acinetobacter species (18), Citrobacter freundii (19), Providencia rettgeri (20), and a Klebsiella species (GenBank accession number CP010390.1) of human origin. A 2-nucleotide deletion was observed in the aphA6 gene located in pHNEC55 and pHNEC46, resulting in early termination of the protein and inactivation of this gene product; this observation was therefore consistent with the loss of the aminoglycoside resistance phenotype in E. coli strains carrying these two plasmids (see Table S1 in the supplemental material).…”

Section: Figmentioning

confidence: 86%

IncFII Conjugative Plasmid-Mediated Transmission of bla _NDM-1 Elements among Animal-Borne Escherichia coli Strains

Lin

Xie

et al. 2017

Antimicrob Agents Chemother

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This study aims to investigate the prevalence and transmission dynamics of the bla NDM-1 gene in animal Escherichia coli strains. Two IncFII bla NDM-1 -encoding plasmids with only minor structural variation in the MDR region, pHNEC46-NDM and pHNEC55-NDM, were found to be responsible for the transmission of bla NDM-1 in these strains. The bla NDM-1 gene can be incorporated into plasmids and stably inherited in animal-borne E. coli strains that can be maintained in animal gut microflora even without carbapenem selection pressure.

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Section: Figmentioning

confidence: 86%

IncFII Conjugative Plasmid-Mediated Transmission of bla _NDM-1 Elements among Animal-Borne Escherichia coli Strains

Lin

Xie

et al. 2017

Antimicrob Agents Chemother

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“…Data obtained from sequencing of the sil gene PCR products from SRKP and SREC suggested that the sil gene homologues contained in these isolates differ from those of pMG101. Sequence analysis suggested that the sil genes from SRKP more likely originated from a plasmid such as pKPN3, one of many IncFII-type resistance plasmids commonly found in the K. pneumoniae taxid (31). From a clinical perspective, IncFII plasmids are persistent, highly mobile genetic elements that have the capability to spread sil gene homologs.…”

Section: Discussionmentioning

confidence: 99%

Unprecedented Silver Resistance in Clinically Isolated Enterobacteriaceae: Major Implications for Burn and Wound Management

Finley

Norton²,

Austin

et al. 2015

Antimicrob Agents Chemother

100

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bIncreased utilization of inorganic silver as an adjunctive to many medical devices has raised concerns of emergent silver resistance in clinical bacteria. Although the molecular basis for silver resistance has been previously characterized, to date, significant phenotypic expression of these genes in clinical settings is yet to be observed. Here, we identified the first strains of clinical bacteria expressing silver resistance at a level that could significantly impact wound care and the use of silver-based dressings. Screening of 859 clinical isolates confirmed 31 harbored at least 1 silver resistance gene. Despite the presence of these genes, MIC testing revealed most of the bacteria displayed little or no increase in resistance to ionic silver (200 to 300 M Ag ؉ ). However, 2 isolates (Klebsiella pneumonia and Enterobacter cloacae) were capable of robust growth at exceedingly high silver concentrations, with MIC values reaching 5,500 M Ag ؉ . DNA sequencing of these two strains revealed the presence of genes homologous to known genetic determinants of heavy metal resistance. Darkening of the bacteria's pigment was observed after exposure to high silver concentrations. Scanning electron microscopy images showed the presence of silver nanoparticles embedded in the extracellular polymeric substance of both isolates. This finding suggested that the isolates may neutralize ionic silver via reduction to elemental silver. Antimicrobial testing revealed both organisms to be completely resistant to many commercially available silver-impregnated burn and wound dressings. Taken together, these findings provide the first evidence of clinical bacteria capable of expressing silver resistance at levels that could significantly impact wound management.

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“…Similar associations were subsequently found between intact or truncated IS1071 copies and different catabolic gene clusters from a variety of bacterial isolates (5,14). Another "transposition module-only" Tn3-family transposon, IS3000, was identified adjacent to the extended-spectrum β-lactamase CTX-M-9 gene from enterobacteria, and more recently, on the multi-resistance Klebsiella pneumoniae plasmid pKp11-42 (15,16).…”

Section: Transposition Module-only Tn3-family Elementsmentioning

confidence: 99%

The Tn 3 -family of Replicative Transposons

Lambin²,

et al. 2015

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Transposons of the Tn3 family form a widespread and remarkably homogeneous group of bacterial transposable elements in terms of transposition functions and an extremely versatile system for mediating gene reassortment and genomic plasticity owing to their modular organization. They have made major contributions to antimicrobial drug resistance dissemination or to endowing environmental bacteria with novel catabolic capacities. Here, we discuss the dynamic aspects inherent to the diversity and mosaic structure of Tn3-family transposons and their derivatives. We also provide an overview of current knowledge of the replicative transposition mechanism of the family, emphasizing most recent work aimed at understanding this mechanism at the biochemical level. Previous and recent data are put in perspective with those obtained for other transposable elements to build up a tentative model linking the activities of the Tn3-family transposase protein with the cellular process of DNA replication, suggesting new lines for further investigation. Finally, we summarize our current view of the DNA site-specific recombination mechanisms responsible for converting replicative transposition intermediates into final products, comparing paradigm systems using a serine recombinase with more recently characterized systems that use a tyrosine recombinase.

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Complete sequences of a novel blaNDM-1-harbouring plasmid from Providencia rettgeri and an FII-type plasmid from Klebsiella pneumoniae identified in Canada

Abstract: pPrY2001 differed from all known plasmids due to its novel backbone and repB. pKp11-42 was similar to IncFIIk plasmids and contained a number of genes that aid in plasmid persistence.

Cited by 42 publications

References 19 publications

IncFII Conjugative Plasmid-Mediated Transmission of bla _NDM-1 Elements among Animal-Borne Escherichia coli Strains

IncFII Conjugative Plasmid-Mediated Transmission of bla _NDM-1 Elements among Animal-Borne Escherichia coli Strains

Unprecedented Silver Resistance in Clinically Isolated Enterobacteriaceae: Major Implications for Burn and Wound Management

The Tn 3 -family of Replicative Transposons

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Complete sequences of a novel blaNDM-1-harbouring plasmid from Providencia rettgeri and an FII-type plasmid from Klebsiella pneumoniae identified in Canada

Abstract: pPrY2001 differed from all known plasmids due to its novel backbone and repB. pKp11-42 was similar to IncFIIk plasmids and contained a number of genes that aid in plasmid persistence.

Cited by 42 publications

References 19 publications

IncFII Conjugative Plasmid-Mediated Transmission of bla NDM-1 Elements among Animal-Borne Escherichia coli Strains

IncFII Conjugative Plasmid-Mediated Transmission of bla NDM-1 Elements among Animal-Borne Escherichia coli Strains

Unprecedented Silver Resistance in Clinically Isolated Enterobacteriaceae: Major Implications for Burn and Wound Management

The Tn 3 -family of Replicative Transposons

Contact Info

Product

Resources

About

IncFII Conjugative Plasmid-Mediated Transmission of bla _NDM-1 Elements among Animal-Borne Escherichia coli Strains

IncFII Conjugative Plasmid-Mediated Transmission of bla _NDM-1 Elements among Animal-Borne Escherichia coli Strains