Complete Whole-Genome Sequences of Two Raoultella terrigena Strains, NCTC 13097 and NCTC 13098, Isolated from Human Cases

Fazal, Mohammed-Abbas; Alexander, Sarah; Grayson, Nicholas; Deheer-Graham, Ana; Oliver, Karen; Holroyd, Nancy; Parkhill, Julian; Russell, Julie E.

doi:10.1128/mra.00239-19

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…The results also showed that the PCM8 strain had 393 subsystems with a coding sequence of 5513 (0,096%) and 117 RNA. This result is close to the results of the research of Fazal et al (2019) which states that the R. terrigena strain NCTC 13097 has a nucleotide base of 5.574.669 bp and a GC content of 57,3% with 5,386 coding sequences and 119 RNA genes. Even so, this study has not analyzed the genomic function of R. terrigena strain NCTC 13097 so it is not known whether this strain has the acdS gene.…”

Section: Ani Value Analysis Results and Wgs Annotations Of The Acds Gensupporting

confidence: 90%

Endophytic bacteria from paddy with double 1-aminocyclopropane-1-carboxylic acid deaminase and nitrogenase activity

Simarmata,

Ngadiman,

Rohman

et al. 2023

Emir J Food Agric

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Plant growth promoting bacteria with dual activity, 1-aminocyclopropane-1-carboxylic-acid deaminase (ACCD) and nitrogenase, is more effective in supporting plant growth under stress condition. Previously, we were obtained several endophytic bacterial strains that exhibited dual activity, one of which was Raoultella terrigena PCM8. This study aimed to characterize the ACCD and nitrogenase genes of PCM8 strain. The acdS gene was obtained from the results of Whole Genomic Sequencing analyis, while the nifH gene was obtained by PCR. The characterization of both of the genes was carried out by means of in-silico analysis. WGS annotation analysis, showed that the acdS gene of PCM8 was located at the locus 19090 of genomic DNA and contains 978 nucleotides. In silico analysis of both acdS and nifH gene products showed that the ACCD enzyme of PCM8 had 325 amino acids, with molecular weight of 34.95 kDa, while nitrogenase as represented by nifH subunit product consist of 96 amino acids with molecular weight of 93.98 kDa, respectively. The ACCD had pI value of 5.06, and catalytic residues of Lys51, Ser78, Tyr287, and Thr288, while nifH gene product had the pI value of 11.77. The results suggested that R. terrigena PCM8 potentially produce double activity of ACCD and nitrogenase and therefore it can be a good candidate as plant growth promoting under stress condition. Keywords: acdS gene, 1-aminocyclopropane-1-carboxylic acid deaminase, endophytic bacteria, nifH gene, nitrogenase Raoultella terrigena

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Section: Ani Value Analysis Results and Wgs Annotations Of The Acds Gensupporting

confidence: 90%

Endophytic bacteria from paddy with double 1-aminocyclopropane-1-carboxylic acid deaminase and nitrogenase activity

Simarmata,

Ngadiman,

Rohman

et al. 2023

Emir J Food Agric

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“…Endophytes as a ubiquitous associate of the plant are considered to play a pivotal role in regulating the primary and secondary metabolism of their host plant, and in providing elicitors to improve plant health, ameliorating stress tolerance in plants and source of therapeutically important secondary metabolites ( Pandey et al, 2022 ). R. terrigena is considered to be an environmental bacterium, which is isolated mainly from soil and aquatic environments ( Fazal et al, 2019 ). It is also isolated from plant roots of Nicotiana tabacum and switchgrass Panicum virgatum , and considered to be an endophytic nitrogen-fixing bacterium ( Schicklberger et al, 2015 ; Gushgari-Doyle et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Plant quercetin degradation by gut bacterium Raoultella terrigena of ghost moth Thitarodes xiaojinensis

2022

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Associated microbes of several herbivorous insects can improve insect fitness. However, the contribution of specific insect gut bacterium to plant toxin toxification for its host fitness remains scarce. Here, a gut bacterium Raoultella terrigena from the ghost moth Thitarodes xiaojinensis larvae was identified. This bacterium grew unhindered in the presence of Polygonum viviparum, which is a natural food for ghost moth larvae but showed significant growth inhibition and toxicity against Spodoptera litura. S. litura reared on artificial diets containing 5, 15 and 25% P. viviparum powder after 7 days coculture with R. terrigena were found to have shorter larval and pupal durations than on the diets containing P. viviparum powder but without R. terrigena coculture. HPLC analysis revealed that the content of quercetin in mineral medium containing 15% P. viviparum powder after 7 days coculture with R. terrigena was significantly decreased (79.48%) as compared with that in P. viviparum powder without R. terrigena coculture. In vitro fermentation further verified that R. terrigena could degrade 85.56% quercetin in Lucia-Bertani medium. S. litura reared on artificial diets containing 0.01, 0.05 and 0.1 mg/g quercetin after 48 h coculture with R. terrigena were also found to have shorter larval, prepupal and pupal durations, as well as higher average pupal weight and adult emergence rate than on the diets containing quercetin, but without R. terrigena coculture. In addition, R. terrigena was detected in the bud and root tissues of the sterilized P. viviparum, indicating that T. xiaojinensis larvae might acquire this bacterium through feeding. These results demonstrate that the gut bacteria contribute to the degradation of plant toxic molecules to improve the development of herbivorous insects and provide fundamental knowledge for developing effective methods for beneficial insect rearing and pest control.

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“…One of the strains also contained an 86 kb plasmid. Bioinformatic analysis of the R. terrigena genome identified 5500 protein‐coding sequences in addition to 85 tRNA and 25 rRNA sequences 21 …”

Section: Discussionmentioning

confidence: 99%

Raoultellaterrigena: Current state of knowledge, after two recently identified clinical cases in Eastern Europe

Lekhniuk

Фесенко

Pidhirnyy

et al. 2021

Clinical Case Reports

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Complete Whole-Genome Sequences of Two Raoultella terrigena Strains, NCTC 13097 and NCTC 13098, Isolated from Human Cases

Cited by 3 publications

References 12 publications

Endophytic bacteria from paddy with double 1-aminocyclopropane-1-carboxylic acid deaminase and nitrogenase activity

Endophytic bacteria from paddy with double 1-aminocyclopropane-1-carboxylic acid deaminase and nitrogenase activity

Plant quercetin degradation by gut bacterium Raoultella terrigena of ghost moth Thitarodes xiaojinensis

Raoultellaterrigena: Current state of knowledge, after two recently identified clinical cases in Eastern Europe

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