Complex alpha and beta mannan foraging by the human gut bacteria

Panwar, Deepesh; Shubhashini, A.; Kapoor, M.

doi:10.1016/j.biotechadv.2023.108166

Cited by 8 publications

(2 citation statements)

References 126 publications

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“…The fourth novel large phage caudovirus D_HF5_2C (OR148986) is predicted to infect bacteria in the Bacillota phylum ( Figure 7 ). Bacillota, a phylum comprised of over 200 bacterial genera, has been found to consistently be one of the dominant groups in many human gut microbiome studies [ 77 , 78 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Diverse Anelloviruses, Cressdnaviruses, and Bacteriophages in the Human Oral DNA Virome from North Carolina (USA)

Paietta,

Kraberger,

Custer

et al. 2023

Viruses

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The diversity of viruses identified from the various niches of the human oral cavity—from saliva to dental plaques to the surface of the tongue—has accelerated in the age of metagenomics. This rapid expansion demonstrates that our understanding of oral viral diversity is incomplete, with only a few studies utilizing passive drool collection in conjunction with metagenomic sequencing methods. For this pilot study, we obtained 14 samples from healthy staff members working at the Duke Lemur Center (Durham, NC, USA) to determine the viral diversity that can be identified in passive drool samples from humans. The complete genomes of 3 anelloviruses, 9 cressdnaviruses, 4 Caudoviricetes large bacteriophages, 29 microviruses, and 19 inoviruses were identified in this study using high-throughput sequencing and viral metagenomic workflows. The results presented here expand our understanding of the vertebrate-infecting and microbe-infecting viral diversity of the human oral virome in North Carolina (USA).

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Section: Resultsmentioning

confidence: 99%

Characterization of Diverse Anelloviruses, Cressdnaviruses, and Bacteriophages in the Human Oral DNA Virome from North Carolina (USA)

Paietta,

Kraberger,

Custer

et al. 2023

Viruses

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show abstract

“…Depending on their sources, these β-mannans have been categorized into four subtypes; linear β-mannan, galactomannan, glucomannan and galactoglucomannan (6). Norway spruce wood-derived galactoglucomannan, for instance, was developed as an MDF in weaning piglets and was shown to be highly selective for Roseburia intestinalis as well as (6)(7)(8)(9). Despite preliminary indications via taxonomic surveys that Carnobacterium, Roseburia and Faecalibacterium spp.…”

Section: Observationmentioning

confidence: 99%

The need for high-resolution gut microbiome characterization to design efficient strategies for sustainable aquaculture production

Gupta,

de León,

Kodama

et al. 2024

Preprint

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Microbiome-directed dietary interventions such as microbiota-directed fibers (MDFs) have a proven track record in eliciting responses in beneficial gut microbes and are increasingly being promoted as an effective strategy to improve animal production systems. Here we used initial metataxonomic data on fish gut microbiomes as well as a wealth of a priori mammalian microbiome knowledge on α-MOS and β-mannan-derived MDFs to study effects of such feed supplements in Atlantic salmon (Salmo salar) and their hitherto poorly characterized gut microbiomes. Our multi-omic analysis revealed that the investigated MDFs (two α-mannans and an acetylated β-galactoglucomannan), at a dose of 0.2%, had negligible effects on both host gene expression, and gut microbiome structure and function under studied conditions. While a subsequent trial using a higher (4%) dietary inclusion of β-mannan significantly shifted the gut microbiome composition, there were still no biologically relevant effects on salmon metabolism and physiology. Only a singleBurkholderia-Caballeronia-Paraburkholderia(BCP) population demonstrated consistent and significant abundance shifts across both feeding trials, although with no evidence of β-mannan utilization capabilities or changes in gene transcripts for producing metabolites beneficial to the host. In light of these findings, we revisited our omics data to predict and outline novel and potentially beneficial endogenous lactic acid bacteria that should be targeted with future, conceivably more suitable, MDF strategies for salmon.IMPORTANCEThis study focuses on the potential of MDFs to improve aquaculture production. Despite preliminary 16S rRNA amplicon data suggested that populations in the salmon gut microbiome could utilize structurally complex mannans, our findings indicates that endogenous microbes could not metabolize it, nor the host responds to its dietary inclusion, at least not under the trial conditions investigated in this study. We highlight that high-resolution and host-specific microbiome characterization can greatly improve trial design and selection of candidate MDFs for future nutritional interventions. Understanding the intricate interplay between host and its gut microbiome is paramount in studies seeking to leverage endogenous microbial communities to benefit the host. While each new condition, whether it is a disease onset or a nutritional stressor, has the potential to profoundly reshape the microbial diversity, composition and outputs, the functional microbiome information gained under healthy conditions represent a pivotal step towards designing more effective trials involving microbiome-reprogramming feed additives. Overall, we envisage that these results will lead to improved focus on coupling fundamental microbiome characterization to the design of next-generation feeds for salmon aquaculture.

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Application of two crucial hemicellulolytic enzymes in fish diet: Xylanase and mannanase

yan Wang,

li Ding,

sheng Zhang

et al. 2025

Animal Feed Science and Technology

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Complex alpha and beta mannan foraging by the human gut bacteria

Cited by 8 publications

References 126 publications

Characterization of Diverse Anelloviruses, Cressdnaviruses, and Bacteriophages in the Human Oral DNA Virome from North Carolina (USA)

Characterization of Diverse Anelloviruses, Cressdnaviruses, and Bacteriophages in the Human Oral DNA Virome from North Carolina (USA)

The need for high-resolution gut microbiome characterization to design efficient strategies for sustainable aquaculture production

Application of two crucial hemicellulolytic enzymes in fish diet: Xylanase and mannanase

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