Complex Biochemical Analysis of Fruiting Bodies from Newly Isolated Polish <i>Flammulina velutipes</i> Strains

Osińska-Jaroszuk, Monika; Jaszek, Magdalena; Sulej, Justyna; Stefaniuk, Dawid; Urbaniak, Monika; Siwulski, Marek; Janusz, Grzegorz

doi:10.5604/17331331.1215609

Cited by 4 publications

(3 citation statements)

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“…This family is involved in β-glucan assembly in Saccharomyces and has putative signaling roles through an interaction with MAP kinases 29 . Although generally linked to cellulose degradation 30,31 , expansins, lytic polysaccharide monooxygenases and cellobiose dehydrogenases have recently been shown to target chitin polymers 32,33 or are expressed in fruiting bodies of Pycnoporus 34 and Flammulina 35 , suggesting a role in fruiting body development. In addition, developmental expression of two alginate lyase-like families (Table 1) were shared by 6 species and that of a β-glucuronidase (GH79_1) was shared by 4 species (Armillaria, Coprinopsis, Rickenella and Lentinus ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Krizsán

Almási

Merényi

et al. 2018

Preprint

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We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall (FCW) remodeling, targeted protein degradation, signal transduction, adhesion and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, protein kinases and cadherin-like proteins, showed massive expansions in Agaricomycetes, with many convergently expanded in multicellular plants and/or animals too, reflecting broad genetic convergence among independently evolved complex multicellular lineages. This study provides a novel entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Krizsán

Almási

Merényi

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…This family is involved in β-glucan assembly in Saccharomyces and has putative signaling roles through an interaction with MAP kinases (32). Although generally linked to cellulose degradation (33,34), expansins, lytic polysaccharide monooxygenases, and cellobiose dehydrogenases have recently been shown to target chitin polymers (35,36) or to be expressed in fruiting bodies of Pycnoporus (37) and Flammulina (38), suggesting a role in fruiting body development. In addition, developmental expression of two alginate lyase-like families (SI Appendix, Table S2) were shared by six species, while that of a β-glucuronidase (GH79 1) was shared by four species (Armillaria, Coprinopsis, Rickenella, and Lentinus).…”

Section: Signatures As Shown Inmentioning

confidence: 99%

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi

Krizsán

Almási

Merényi

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

131

364

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The evolution of complex multicellularity has been one of the major transitions in the history of life. In contrast to simple multicellular aggregates of cells, it has evolved only in a handful of lineages, including animals, embryophytes, red and brown algae, and fungi. Despite being a key step toward the evolution of complex organisms, the evolutionary origins and the genetic underpinnings of complex multicellularity are incompletely known. The development of fungal fruiting bodies from a hyphal thallus represents a transition from simple to complex multicellularity that is inducible under laboratory conditions. We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall remodeling, targeted protein degradation, signal transduction, adhesion, and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, expansin-like proteins, protein kinases, and transcription factors, showed expansions in Agaricomycetes, many of which convergently expanded in multicellular plants and/or animals too, reflecting convergent solutions to genetic hurdles imposed by complex multicellularity among independently evolved lineages. This study provides an entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms. complex multicellularity | evolution | fungi | comparative genomics | fruiting body development F ungi represent a diverse lineage of complex multicellular organisms with a unique evolutionary history compared with complex multicellular animals, embryophytes, florideophytes, and laminarean brown algae (1-4). Within the fungal kingdom, complex multicellularity is discussed mostly in the context of fruiting bodies, which are found in at least eight independent lineages (2), of which the Pezizomycotina (Ascomycota) and the Agaricomycetes (Basidiomycota) contain the vast majority of species. The mushroom-forming fungi (Agaricomycetes) comprise >21,000 species and originated 350 million years ago (5), approximately coinciding with the origin of tetrapods. Fruiting bodies of mushroom-forming fungi have immense importance in agriculture, ecology, and medicine; they represent an important and sustainable food source, with favorable medicinal properties (e.g., antitumor, immunomodulatory) (6). Mushroom-forming fungi share a single origin of fruiting body formation that probably dates to the most recent common ancestor of the Agaricomycetes, Dacrymycetes, and Tremellomycetes (2).Fruiting body development in mushroom-forming fungi has been subject to surprisingly few studies (see, e.g., refs. 7-10), result...

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“…Пастеризацію варіантів субстратів проводили за наступним алгоритмом: А) Складали варіанти формул субстратів відповідно до даних попередніх дослідників, але з урахуванням особливостей місцевої сировини [19][20][21]. Було зроблено 8 варіантів:…”

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Technological principles for the introduction of winter mushroom (Flammulina velutipes (Curtis) Singer) into the industrial culture

Bandura¹,

Bisko²,

Кулик³

et al. 2020

Naukovì dopovìdì Nacìonalʹnogo unìversitetu bìoresursiv ì priro

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Flammulina velutipes - enokitake or shortly - "enoki", is one of the most widely cultivated mushrooms on the planet. The value of the fruit bodies of the “gold mushroom” is determined by their high functional properties, in particular, the presence of bio-active proteins FIP-fve (fungal immunomodulatory protein), and special polysaccharides. Consumers like its delicate texture and bright rich aroma. In Ukraine, this mushroom is successfully grown in small-scale production, but the technological foundations for introducing it into industrial culture have not been developed until current time. The aim of the work was to determine the effective technical operations of the Flammulina industrial production using local agricultural wastes. The substrate formula (sawdust 400 g; straw 400 g; wheat bran 180g; chalk (CaCO3) 20g; water 1850 g) after sterilization (under 121°C for 120 minutes) was used for the cultivation. Ten strains from the IBK mushroom culture collection were checked, and three strains: 2038 (white race), 2039 and 2337 (yellow race), were selected. These strains have shown the best technological characteristics – short harvesting time (38 days for 2039 and 2037, 45 days for 2038) and the high level of biological efficiency (2037 -52,6%, 2039-51,3%, 2038-45,4%) in comparison with strain 1994 (white race) with the worse indexes (62 days of fruiting and 25,7% of biological efficiency) in the screening experiment. In addition, any bacterial diseases did not detect during cultivation process, except strain 1880. In the second part of the experiment selected strains have been grown in industrial conditions and their main technical characteristics were studied: biological efficiency and the time of first flush harvesting on 8 substrate compositions from local agricultural wastes. Pellets from sunflower husks as a substrate ingredient has had some technical advantages: the time of substrate preparation was shorter because it was soaked in a few minutes and the necessary density was reached. The statistical analyses data show significant differences in the parameters of vegetative growth time as for one strain on the different substrate composition as between strains. The best overall rate of vegetative development rate (U-test) was obtained in strain 2337. The transition to the generative stage was the shortest when the substrate of formula 8 was used (2337 and 2039 with a term of 27 and 28 days, respectively). The lowest time was recorded in treatment 2 (39-42 days for these strains). The most biological efficiency index (81,2%) was noted on substrate composition 8, and the lowest (35,4%) on formula 2 in strain 2039. The selected substrate formula 8 (sunflower husk 400 g, pellets of sunflower husk 300 g, corn bran 200 g, rapeseed 90 g, chalk 10 g, water 1800 g) was used for the estimation of effect weight of substrate bag to the biological efficiency of strain 2039. As a result, the biological efficiency of 2039 strain was in 1,6 times more in case of using smaller bags with 1500 g weight in comparison with treatment of bigger bag with weight 3000 g, 121,2±17,3% and 75,8±9,4% respectively. However, statistical analyses did not reveal the effect of weight for first harvesting time of 2039 strain (no statistical differences between 39±3 and 44±2 days for 1500 g and 3000 g bag weight respectively). F. velutipes strains 2037, 2039 (yellow race) and 2038 (white race) can be recommended for cultivation, but according to our research, the further studies of local agro wastes using, the influence of micro-climate on the growing process, morphological characteristics and biochemical content are importantly necessary for the development of successful industrial production.

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Complex Biochemical Analysis of Fruiting Bodies from Newly Isolated Polish Flammulina velutipes Strains

Cited by 4 publications

References 58 publications

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Transcriptomic atlas of mushroom development highlights an independent origin of complex multicellularity

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi

Technological principles for the introduction of winter mushroom (Flammulina velutipes (Curtis) Singer) into the industrial culture

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