Complex equilibria in capillary zone electrophoresis and their use for the separation of rare earth metal ions

Vogt, Carsten; Conradi, S.

doi:10.1016/0003-2670(94)80189-4

Cited by 48 publications

(22 citation statements)

References 15 publications

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“…A very good separation of 13 lanthanides together with some alkali, alkaline earth and transition metals was achieved also in the lactate electrolyte. Systematic investigations of effects of the HIBA concentration, the pH value, and the presence of secondary complexing ligand were performed by Vogt and Conradi [48]. It was proven that it is the combination of ligands possessing different abilities for the complexation of lanthanide ions (like HIBA and acetate) that leads to a better separation as opposed to the combination of ligands with similar complexing behavior (like HIBA and lactate).…”

Section: Separation Modes and Strategiesmentioning

confidence: 99%

Analytical separations of lanthanides and actinides by capillary electrophoresis

Janoš

2003

Electrophoresis

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The separation of lanthanide and actinide elements belongs to one of the most challenging tasks of the separation science, due to a great similarity in their physical and chemical properties. The electrophoretic separation can be accomplished in the presence of suitable complex-forming agents, from which alpha-hydroxyisobutyric acid (HIBA) has been used most often. In the most effective capillary electrophoretic mode--capillary zone electrophoresis (CZE)--a complete separation of lanthanide ions can be accomplished within a few minutes. Various electrophoretic methods can be relatively easily adopted for the determinations of individual lanthanide elements in certain kinds of technical materials, concentrates, precursors, etc., where the high speed and low costs of analysis characteristics of capillary electrophoresis (CE) may be advantageously exploited. Electrophoretic techniques may also be employed for speciation studies, especially for examinations of the behavior of actinides in the environment.

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Section: Separation Modes and Strategiesmentioning

confidence: 99%

Analytical separations of lanthanides and actinides by capillary electrophoresis

Janoš

2003

Electrophoresis

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“…In addition to these techniques, applications of capillary electrophoresis for Ianthanide analysis have appeared recently (13,14). Capillary electrophoretic separations rely on differences in the electrophoretic mobility of analyte species in an electrolyte buffer while under the influence of an applied electric field.…”

Section: Description Of the Methodsmentioning

confidence: 99%

Analytical-Scale Separations of the Lanthanides: A Review of Techniques and Fundamentals

Nash

Jensen

2001

Separation Science and Technology

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MEDM, which is derived from Motif Editor and DisplayManager,is the primarygraphicaluser interface to the EPICScontrolsystemand has also been used for other control systems. IvlEDMhas two modes of operation,EDIT and EXECUTE. In its EDIT mode it provides the drawing tools needed to design control screensfor operatorinterfaces. In its EXECUTEmode it manages those screens to communicate with the control system. MEDM provides a set of interface objects that falls into three main categories: (1) Monitors, suchas text,meters,and plots;(2) Controllers, such as buttons,menus, and sliders; and (3) Drawing Objects,such as lines,rectangles,and images. Each of these objects has many options, allowing for the developmentof screens ranging from simple to quite sophisticated.MEDMhas been developedover the last decade,primarilyat ArgonneNationalLaboratory,and is a large,welltested,extensivelyusedprogram. It runs on most flavors of UNIX, VMS, and Windows 951981NT.It has been used to design thousandsof controlscreens,such as the one shownin Fig. 1, at the AdvancedPhoton Source and other sites around the world. This paper presentsan overviewof MEDMand its features.

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“…In the literature, capillary electrophoresis is used to study the kinetics of several complex formation of lanthanide ions with chelating ligands, 24 or to separate and determine rare earth ions by using different kinetics of each ion in the electrophoresis. 25,26 The use of a lanthanide chelate for biomolecules as a luminescent tag in capillary gel electrophoresis poses several obstacles to overcome that are specific to lanthanide chelates, as compared to conventional organic dye labels, i.e., the high electric voltage used in electrophoresis might cause dissociation of the positively charged metal ion from the chelate ligands. The second problem is that the mobility of the labeled proteins might be disturbed under an electric field by such a heavy label, like DTBTA-Eu 3+ with a negative charge (-1) as a whole, and the protein ladder commonly used to deduce the molecular weight of unknown proteins may not hold.…”

Section: Introductionmentioning

confidence: 99%

“…Previously, a few analyses of several lanthanide complexes by capillary electrophoresis had been reported, but in those systems the metal ion was complexed with a ligand either on the column with a free ligand added in the carrier buffer to avoid metal dissociation, [24][25][26][27][28][29] or a metal ion was introduced after capillary electrophoresis in order to avoid metal dissociation during electrophoresis. 30 Early in lanthanide chelate research, a Tb chelate complex with a ligand obtained from the reaction of sodium p-aminosalicylate with diethyltriamine pentaacetic acid (DTPA) dianhydride was reported to be a stable Tb chelate label for DNA in gel electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

Sodium Dodecyl Sulfate Polyacrylamide Slab Gel Electrophoresis and Hydroxyethyl Cellurose Gel Capillary Electrophoresis of Luminescent Lanthanide Chelate-labeled Proteins with Time-Resolved Detection

et al. 2009

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Proteins labeled with a luminescent lanthanide chelate, {{2,2¢,2≤,2¢¢¢-{4¢-{[(4,6-dichloro-1,3,5-triazin-2-yl)amino]biphenyl-4-yl}-2,2¢:6¢,2≤-terpyridine-6,6≤-diyl}bis(methylenenitrilo)}tetrakis(acetato)}europium(III) (DTBTAEu 3+ ), were analyzed with sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) slab gel electrophoresis and hydroxyethyl cellurose gel capillary electrophoresis with a time-resolved fluorometric detector. The metal ion of the luminescent lanthanide chelate did not dissociate from the ligand during electrophoresis, and the luminescence was retained. On a slab gel, the band of DTBTA-Eu 3+ -labeled streptavidin was apparently less broad than that of AlexaFluor 488-labeled streptavidin. DTBTA-Eu 3+ in SDS-PAGE slab gel is stable, and the gel after electrophoresis can be dried and stored for at least one year without luminescence fading. In capillary gel electrophoresis (CGE), five labeled proteins with different molecular weight were separated, and a good correlation was observed between the molecular weight and the migration time. Although the detection limits of these proteins determined in buffer solutions of the capillary electrophoresis were not better than those reported for CGE with laser-induced-detection, the detection limits of the same proteins in the present CGE system were not significantly deteriorated in serum solutions compared to those in buffer solutions, and the advantage of using time-resolved detection has been shown.

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Complex equilibria in capillary zone electrophoresis and their use for the separation of rare earth metal ions

Cited by 48 publications

References 15 publications

Analytical separations of lanthanides and actinides by capillary electrophoresis

Analytical separations of lanthanides and actinides by capillary electrophoresis

Analytical-Scale Separations of the Lanthanides: A Review of Techniques and Fundamentals

Sodium Dodecyl Sulfate Polyacrylamide Slab Gel Electrophoresis and Hydroxyethyl Cellurose Gel Capillary Electrophoresis of Luminescent Lanthanide Chelate-labeled Proteins with Time-Resolved Detection

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