Complex formation and reciprocal regulation between GSK3β and C3G

Sriram, Divya; Dayma, Kunal; Devi, Ambure Sharada; Raghawan, Akhouri Kishore; Rawat, Shivali; Radha, Vegesna

doi:10.1016/j.bbamcr.2021.118964

Cited by 3 publications

(3 citation statements)

References 94 publications

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“…RAP1 primarily interacts with an a-helix in RAPGEF1 formed by residues 788 to 826. This region encompasses the NES and GID identified by us earlier [9, 15].…”

Section: Resultsmentioning

confidence: 99%

“…Residues in the REM region identified for interaction with the NTD, are E740 & E794 (in isoform 3) of mouse. E794 is within the region identified by us earlier as the nuclear export sequence and also GSK3β interaction domain [9, 15]. It appears that residues in the REM domain serve multiple functions, and its interaction with NTD may regulate nuclear export, as well as interaction with other molecules like GSK3β.…”

Section: Discussionmentioning

confidence: 99%

“…Residues in the N-terminal interact with Ras exchanger motif within the catalytic domain to enhance its activity. RAPGEF1 interacts with and is phosphorylated by Src family kinases, Abl, and GSK3, which regulate its function, and sub-cellular localization [7][8][9]. It also forms complexes with Crk, Cas, -catenin, cenexin, and TC-PTP [4,[10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Development and tissue specific expression of RAPGEF1 (C3G) transcripts having exons encoding disordered segments with predicted regulatory function

Verma,

Goel,

Koner

et al. 2024

Preprint

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The ubiquitously expressed RAPGEF1(C3G), regulates differentiation, and is essential for development of mouse embryos. While multiple transcripts have been predicted, evidence of their expression and function is scarce. We demonstrate tissue and development specific expression of novel transcripts with exons 12-14 in various combinations, in the mouse. These exons encode an intrinsically disordered serine-rich polypeptide, that undergoes phosphorylation. Isoform switching occurred during differentiation of myoblasts and mouse embryonic stem cells. In silico structure and docking studies indicated that the additional exons alter intra-molecular interactions keeping it in a closed confirmation, and interaction with its target, RAP1A. Our results demonstrate the expression of novel RAPGEF1 isoforms and suggest cassette exon inclusion as an additional means of regulating RAPGEF1 activity during differentiation.

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“…RAP1 primarily interacts with an a-helix in RAPGEF1 formed by residues 788 to 826. This region encompasses the NES and GID identified by us earlier [9, 15].…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and tissue specific expression of RAPGEF1 (C3G) transcripts having exons encoding disordered segments with predicted regulatory function

Verma,

Goel,

Koner

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Development and tissue specific expression of RAPGEF1 (C3G) transcripts having exons encoding disordered segments with predicted regulatory function

Verma,

Goel,

Koner

et al. 2024

Mol Biol Rep

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Developmental functions ofrapgef1bin neural crest specification and presomitic mesoderm patterning

Prasad,

Iyer,

D’silva

et al. 2024

Preprint

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Rapgef1, a cell fate determinant and effector of multiple signaling events is essential for mammalian embryonic development. Here, we investigated the developmental role of rapgef1 using zebrafish embryos as a model. We show that rapgef1 is maternally expressed and alternately spliced isoforms of its two paralogs rapgef1a and rapgef1b show development and tissue-specific expression. CRISPR-Cas9 and morpholino-based targeting of rapgef1b resulted in developmental defects in the embryonic brain and somites. The rapgef1b morphants showed altered expression of lineage determinants of the cranial neural crest. Transcriptome and in-depth gene expression analysis of morphants revealed fresh insights into the developmental functions of rapgef1b in presomitic mesoderm, and somitogenesis. During early embryonic mitoses, the morphants showed mitotic defects such as diffused spindle poles and chromosome mis-congression. Our results demonstrate that rapgef1b is required for normal embryonic mitoses, cranial neural crest specification, somitogenesis, and myogenesis during embryonic development.

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Complex formation and reciprocal regulation between GSK3β and C3G

Cited by 3 publications

References 94 publications

Development and tissue specific expression of RAPGEF1 (C3G) transcripts having exons encoding disordered segments with predicted regulatory function

Development and tissue specific expression of RAPGEF1 (C3G) transcripts having exons encoding disordered segments with predicted regulatory function

Development and tissue specific expression of RAPGEF1 (C3G) transcripts having exons encoding disordered segments with predicted regulatory function

Developmental functions ofrapgef1bin neural crest specification and presomitic mesoderm patterning

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