Complex formation between uranium(VI) and α-D-glucose 1-phosphate

Koban, Astrid; Geipel, Gerhard; Bernhard, Gert

doi:10.1524/ract.91.7.393.20017

Cited by 12 publications

(16 citation statements)

References 17 publications

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“…We therefore conclude that the UO 2 G6P species shows no fluorescence properties. This observation is similar to those recently described for complexation of uranyl with glucose 1-phosphate [15]. The spectra were corrected for the uranyl hydroxide species, which are present in varying amounts depending on the experimental conditions.…”

Section: Trlfs Measurementssupporting

confidence: 85%

“…The electrode was calibrated for each experimental run with NBS buffers at pH 4.01 and 6.87 (Schott). A detailed description of the titration procedure is given in [15].…”

Section: Potentiometric Titrationmentioning

confidence: 99%

“…To validate the stoichiometry of reaction (2), slope analyses according to mass action law were performed for both systems, at all measured pH values as described in [15]. The concentrations of the free uranyl ion were determined from the measured and deconvoluted fluorescence spectra.…”

Section: Trlfs Measurementsmentioning

confidence: 99%

“…Many studies about the complexation behavior of various metal ions with sugar phosphates have been published [5][6][7][8][9][10][11][12][13][14]. The first investigation of complex formation with actinides was the reaction of glucose 1-phosphate with uranium(VI) [15]. Former studies with simple metal(II) cations describe only complexes with a metal-to-ligand ratio of 1 : 1 [5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…Former studies with simple metal(II) cations describe only complexes with a metal-to-ligand ratio of 1 : 1 [5][6][7][8][9][10][11][12][13][14]. Uranium(VI) forms additionally a 1 : 2 complex [15].…”

Section: Introductionmentioning

confidence: 99%

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Uranium(VI) complexes with sugar phosphates in aqueous solution

Koban¹,

Geipel²,

Roßberg³

et al. 2004

Radiochimica Acta

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Uranium / Sugar phosphate / Complex formation / Potentiometric titration / TRLFS / EXAFSSummary. The complex formation in the aqueous systems of uranium(VI) with glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) were studied using potentiometric titration, TRLFS, and EXAFS. Two complexes with a uranyl-to-ligand ratio of 1 : 1 and 1 : 2 for both sugars were observed. Complex stability constants were determined by potentiometric titration for both complexes to be log β 11 (G6P) = 5.89 ± 0.40, log β 12 (G6P) = 9.45 ± 0.08, log β 11 (F6P) = 5.72 ± 0.21, and log β 12 (F6P) = 9.54 ± 0.09. Stability constants could be determined with TRLFS under the specific experimental conditions only for the 1 : 1 complexes: log β 11 (G6P) = 6.35 ± 0.28, and log β 11 (F6P) = 5.66 ± 0.17.The TRLFS measurements results show the UO 2 G6P complex to exhibit no fluorescence properties. For this system, only a decrease of the fluorescence intensity with increasing ligand concentration was observed. For the UO 2 F6P system, a red shift of the fluorescence emission bands of about 8 to 9 nm compared to the free uranyl ion was observed. The fluorescence emission wavelengths of the UO 2 F6P complex were determined to be 483, 496, 518, 542, and 567 nm, and the lifetime of this complex is 0.13 ± 0.05 µs.Uranium L III -edge EXAFS measurements at pH 3.5, 4.0, and 5.5 yielded a shortened U−O eq bond distance (2.30 ± 0.02 to 2.37 ± 0.02 Å), compared to the UO 2 2+ (H 2 O) 5 ion (2.40 ± 0.02 Å), due to a monodentate coordination via the oxygen atoms of the phosphate group.

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Section: Trlfs Measurementssupporting

confidence: 85%

“…The electrode was calibrated for each experimental run with NBS buffers at pH 4.01 and 6.87 (Schott). A detailed description of the titration procedure is given in [15].…”

Section: Potentiometric Titrationmentioning

confidence: 99%

Section: Trlfs Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Uranium(VI) complexes with sugar phosphates in aqueous solution

Koban¹,

Geipel²,

Roßberg³

et al. 2004

Radiochimica Acta

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Osteopontin: A Uranium Phosphorylated Binding‐Site Characterization

Safi

Creff

Jeanson

et al. 2013

Chemistry A European J

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Herein, we describe the structural investigation of one possible uranyl binding site inside a nonstructured protein. This approach couples spectroscopy, thermodynamics, and theoretical calculations (DFT) and studies the interaction of uranyl ions with a phosphopeptide, thus mimicking a possible osteopontin (OPN) hydroxyapatite growth-inhibition site. Although thermodynamical aspects were investigated by using time-resolved laser fluorescence spectroscopy (TRLFS) and isothermal titration calorimetry (ITC), structural characterization was performed by extended X-ray absorption fine structure (EXAFS) at the U LIII -edge combined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. From the vibrational and fluorescence spectra, several structural models of a UO2 (2+) /peptide complex were developed and subsequently refined by using theoretical calculations to fit the experimental EXAFS obtained. The structural effect of the pH value was also considered under acidic to moderately acidic conditions (pH 1.5-5.5). Most importantly, the uranyl/peptide coordination environment was similar to that of the native protein.

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