2008
DOI: 10.1021/bi800334p
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Complex Formation of Amphotericin B in Sterol-Containing Membranes As Evidenced by Surface Plasmon Resonance

Abstract: Amphotericin B (AmB) is a membrane-active antibiotic that increases the permeability of fungal membranes. Thus, the dynamic process of its interaction with membranes poses intriguing questions, which prompted us to elaborate a quick and reliable method for real-time observation of the drug's binding to phospholipid liposomes. We focused on surface plasmon resonance (SPR) and devised a new modification method of sensor chips, which led to a significant reduction in the level of nonspecific binding of the drug i… Show more

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Cited by 67 publications
(84 citation statements)
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“…Optimization of Immobilization of Model Lipid Membranes on the L1 Sensor Chip In previous studies, 20) the methodology using a modified CM5 sensor chip for the assay of the binding of AmB and the sterol-containing lipid membrane was reported using SPR, and the selectivity of AmB for sterol-containing lipid membrane was shown, indicating that AmB has a higher affinity for ergosterol membrane than cholesterol membrane. In this SPR study, two types of liposomes containing the sterol were used as model lipid membranes.…”
Section: Resultsmentioning
confidence: 99%
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“…Optimization of Immobilization of Model Lipid Membranes on the L1 Sensor Chip In previous studies, 20) the methodology using a modified CM5 sensor chip for the assay of the binding of AmB and the sterol-containing lipid membrane was reported using SPR, and the selectivity of AmB for sterol-containing lipid membrane was shown, indicating that AmB has a higher affinity for ergosterol membrane than cholesterol membrane. In this SPR study, two types of liposomes containing the sterol were used as model lipid membranes.…”
Section: Resultsmentioning
confidence: 99%
“…POPC-cholesterol (4 : 1, v/v) was used to mimic of mammalian membranes, while POPC-ergosterol (4 : 1, v/v) was used as a model fungal membrane. 20) We aimed to establish a better assay of AmB-lipid membrane interaction with our SPR system, considering the time-consuming process for sensor chip modification, the damage to the immobilized liposome on the chip due to repeated and consecutive AmB injection, the carryover between each binding assay, and the throughput and sensitivity. In other words, we tried to establish a high-throughput, sensitive, and reproducible binding assay of AmB-sterol containing lipid membrane using an l1 sensor chip, which is usually used for lipid-membrane systems.…”
Section: Resultsmentioning
confidence: 99%
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“…A POPC/cholesterol (4:1, v/v) mixture was used to mimic mammalian membranes, while a POPC/ ergosterol (4:1, v/v) mixture was used as a fungal membrane system. 18,26 The liposome mixture was prepared using PBS, isotonic dextrose solution, and water for injection in an effort to establish deal conditions to conduct steady liposome capture on the L1 chip. Also, these solutions were chosen because PBS is a typical buffer used for liposome preparation and other solutions are described as solutions for preparation of therapeutic medications in the Ethical Drug Package Insert of Fungizone.…”
Section: Optimization Of Immobilization Of Model Lipid Membranes On Tmentioning
confidence: 99%