The aim of the present study was to develop an optimization procedure for supercritical fluid extraction parameters, in order to obtain the highest possible yield of sugars and cyclitols from plant material. Response surface methodology based on Box-Behnken design was applied to evaluate the effect of: temperature (40, 60, 80 • C), pressure (100, 200, 300 bar), and co-solvent (methanol) percentage (20, 25, 30%). As a result of the optimization process, we found that the highest amount of sugars (15.02 mg/gof dried material) and cyclitols (0.86 mg/g of dried material) was obtained when the following parameters were applied: 80 • C, 228 bar, and 30% of methanol.Moreover, co-solvent concentration and temperature had a higher influence onto the obtained amounts compared with the pressure. K E Y W O R D S chamomile, cyclitols isolation, response surface methodology, sugars isolation, supercritical fluid extraction Article Related Abbreviations: ANOVA, analysis of variance; IS, internal standard; RSM, response surface methodology; SFE, supercritical fluid extraction; UAE, ultrasound-assisted extraction.Several studies reported cyclitols as anti-oxidant, antiinflammatory, and anticancer agents [3][4][5]. Other studies demonstrated that some cyclitols such as D-pinitol and chiroinositol possess insulin-like properties and they can be useful in treating of diabetes by reducing the level of blood glucose [6,7].Chamomile is a traditional herb used for medicinal purposes [8]. It is a member of Asteraceae family, represented by two common varieties viz. German Chamomile (Matricaria recutita L.) and Roman Chamomile (Chamaemelum nobile). The dried flowers are used for preparing the chamomile tea known for its multiple effects like: calming effect of fever, reassuring of nerves and reducer of anxiety of the nightmares, insomnia, and other sleep problems [9]. Moreover, the essential oils of chamomile are used in the pharmaceutical, cosmetic, perfumery, and aromatherapy industries [10,11].The interest in obtaining bioactive compounds from plants for their further uses and applications have motivated the researchers to develop new and efficient methodologies