Complex mixture analysis by two-dimensional mass spectrometry using a miniature ion trap

Szalwinski, Lucas J.; Cooks, R. Graham

doi:10.1016/j.talo.2020.100028

Cited by 15 publications

(11 citation statements)

References 30 publications

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“…For four of the five analytes, an improvement of the signal/noise ratio was observed across three replicates at the 100 ng/mL level. This is expected as previous comparisons between the two instruments using similar scans showed greater sensitivity on the miniature instrument, , a phenomenon mostly attributed to the miniature instrument operating at higher pressure, thereby increasing fragmentation efficiency due to greater momentum transfer into the ion.…”

Section: Resultssupporting

confidence: 59%

“…Previously, we have demonstrated that data-independent MS/MS scans, first implemented using benchtop ion traps, can be used with miniature mass spectrometers to optimize sample use and analysis time. , These MS/MS scans are most readily coupled to miniature mass spectrometers by utilizing a discontinuous atmospheric pressure interface (DAPI), which introduces both ions and neutrals over a small time interval in order to reduce vacuum pump requirements . Instruments utilizing the DAPI require significant pump-down time after sample introduction before mass analysis, thereby increasing the total scan-to-scan time.…”

Section: Introductionmentioning

confidence: 99%

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Novel Ion Trap Scan Modes to Develop Criteria for On-Site Detection of Sulfonamide Antibiotics

Szalwinski

Morato

et al. 2021

Anal. Chem.

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Advances in ambient ionization techniques have facilitated the direct analysis of complex mixtures without sample preparation. Significant attention has been given to innovating ionization methods so that multiple options are now available, allowing for ready selection of the best methods for particular analyte classes. These ambient techniques are commonly implemented on benchtop systems, but their potential application with miniature mass spectrometers for in situ measurements is even more powerful. These applications require that attention be paid to tailoring the mass spectrometric methodology for the on-site operation. In this study, combinations of scan modes are employed to efficiently determine what tandem mass spectrometry (MS/MS) operations are most useful for detecting sulfonamides using a miniature ion trap after ionization. First, mixtures of representative sulfonamide antibiotics were interrogated using a 2D MS/MS scan on a benchtop ion trap in order to determine which class-specific fragments (ionic or neutral) are shared between the sulfonamides and thus have diagnostic value. Then, three less-used combination scans were recorded: (i) a simultaneous precursor ion scan was used to detect both analytes and an internal standard in a single ion injection event to optimize quantitative performance; (ii) a simultaneous precursor/neutral loss scan was used to improve detection limits; and finally, (iii) the simultaneous precursor/neutral loss scan was implemented in a miniature mass spectrometer and representative sulfonamides were detected at concentrations as low as 100 ng/mL by nano-electrospray and 0.5 ng absolute by paper spray ionization, although improvements in the stability of the home-built instrumentation are needed to further optimize performance.

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Section: Resultssupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Novel Ion Trap Scan Modes to Develop Criteria for On-Site Detection of Sulfonamide Antibiotics

Szalwinski

Morato

et al. 2021

Anal. Chem.

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“…Using this approach, we identified the components of mixtures of proscribed drugs. 25 Such experiments have two further advantages for the problem at hand: (i) the MS scan can be performed on the 1 Hz time scale and (ii) with appropriate ionization methods no sample preparation is needed.…”

Section: Introductionmentioning

confidence: 99%

Bacterial growth monitored by two-dimensional tandem mass spectrometry

Szalwinski

Gonzalez

Morato

et al. 2022

Analyst

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“…Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry method that relies on manipulating ion motions to correlate precursor and fragment ion signals [ 1 , 2 , 3 ]. Various 2D MS methods have been developed for quadrupolar ion traps, linear ion traps, and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) [ 4 , 5 , 6 , 7 , 8 ]. In addition to precursor-fragment correlations, two-dimensional partial correlation mass spectrometry (2D PC MS) has also been developed to explore correlations between complementary fragment ion signals [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Phase Correction for Absorption Mode Two-Dimensional Mass Spectrometry

2021

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Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry method that relies on manipulating ion motions to correlate precursor and fragment ion signals. 2D mass spectra are obtained by performing a Fourier transform in both the precursor ion mass-to-charge ratio (m/z) dimension and the fragment ion m/z dimension. The phase of the ion signals evolves linearly in the precursor m/z dimension and quadratically in the fragment m/z dimension. This study demonstrates that phase-corrected absorption mode 2D mass spectrometry improves signal-to-noise ratios by a factor of 2 and resolving power by a factor of 2 in each dimension compared to magnitude mode. Furthermore, phase correction leads to an easier differentiation between ion signals and artefacts, and therefore easier data interpretation.

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Complex mixture analysis by two-dimensional mass spectrometry using a miniature ion trap

Cited by 15 publications

References 30 publications

Novel Ion Trap Scan Modes to Develop Criteria for On-Site Detection of Sulfonamide Antibiotics

Novel Ion Trap Scan Modes to Develop Criteria for On-Site Detection of Sulfonamide Antibiotics

Bacterial growth monitored by two-dimensional tandem mass spectrometry

Phase Correction for Absorption Mode Two-Dimensional Mass Spectrometry

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