2008
DOI: 10.1007/s00424-008-0575-5
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Complex modulation of L-type Ca2+ current inactivation by sorcin in isolated rabbit cardiomyocytes

Abstract: Modulation of the L-type Ca(2+) channel (LTCC) by sorcin was investigated by measuring the L-type Ca(2+) current (I (Ca,L)) in isolated rabbit ventricular myocytes using ruptured patch, single electrode voltage clamp in the absence of extracellular Na(+). Fifty millimolars EGTA (170 nM Ca(2+)) in the pipette solution buffered bulk cytoplasmic [Ca(2+)], but retained rapid Ca(2+)-dependant inactivation of I (Ca,L,). Recombinant sorcin (3 microM) in the pipette significantly slowed time-dependant inactivation (ta… Show more

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Cited by 28 publications
(23 citation statements)
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References 34 publications
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“…Our unbiased analysis of transcriptional changes after mutation of single miR-1/133a clusters revealed the miR-1 target Sorcin [36] to be significantly upregulated at least in miR-1-1/133a-2 knock-out mice and we could confirm this finding at the protein level. Whereas it is established that Sorcin may enhance contractility of cardiomyocytes, it is currently unclear whether Sorcin might also modulate L-type calcium channel activity in vivo [37]. We found moderate upregulation of Sorcin only in one of the models.…”
Section: Discussioncontrasting
confidence: 48%
“…Our unbiased analysis of transcriptional changes after mutation of single miR-1/133a clusters revealed the miR-1 target Sorcin [36] to be significantly upregulated at least in miR-1-1/133a-2 knock-out mice and we could confirm this finding at the protein level. Whereas it is established that Sorcin may enhance contractility of cardiomyocytes, it is currently unclear whether Sorcin might also modulate L-type calcium channel activity in vivo [37]. We found moderate upregulation of Sorcin only in one of the models.…”
Section: Discussioncontrasting
confidence: 48%
“…As previously described [16], a partial rabbit sorcin sequence was amplified using primers based on the known human sorcin sequence by reverse transcription of RNA from a chinchilla bastard rabbit. Sense and antisense oligonucleotides were chosen to target the 19 nucleotide segment complementary to the rabbit sorcin mRNA sequence GCAAGAUCACCUUCGAUGA and ligated downstream of a U6 promoter into vector pSIREN-DNR (BD Biosciences Clontec, Palo Alto, USA).…”
Section: Methodsmentioning
confidence: 99%
“…On the other hand, CDI is reduced by CaBP1, which would enhance Ca 2ϩ entry (6). Dual opposite regulation of VDI and CDI resembles the effect of sorcin (8) and may be physiologically relevant. For Ca V 1.2 channels in ventricular cardiomyocytes, VDI may primarily regulate channel function under basal conditions, whereas CDI is dominant upon ␤-adrenergic stimulation (51,52).…”
Section: Discussionmentioning
confidence: 99%