Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling

Wang, Wensheng; Zhao, Xiuqin; Li, Min; Huang, Liying; Xu, Jianlong; Zhang, Fan; Cui, Yanru; Fu, Binying; Li, Zhikang

doi:10.1093/jxb/erv476

Cited by 118 publications

(74 citation statements)

References 48 publications

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“…The mechanisms of salt tolerance in plants are very complex, which require coordinated expression of multiple stress responsive genes, and their encoding products can directly or indirectly protect plants. In plant, salt stress‐related genes or proteins form a complex regulatory network involving mitogen‐activated protein kinase cascade, reactive oxygen species (ROS) scavenging, defense‐related protein synthesis, glycolysis, tricarboxylic acid cycle, photosynthesis, amino acid metabolism, lipid metabolism and energy generation (Aghaei et al , Sobhanian et al , Zhang et al , Wang et al ). Plant hormones such as abscisic acid, auxin and jasmonic acid are key regulators in plant growth and salt stress response.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Proteomic and physiological responses of Arabidopsis thaliana exposed to salinity stress and N‐acyl‐homoserine lactone

Ding

Cao

Duan

et al. 2016

Physiologia Plantarum

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To evaluate the alleviating action of exogenous N-acyl-homoserine lactones (AHLs) on NaCl toxicity, morphological, physiological and proteomic changes were investigated in Arabidopsis thaliana seedlings. Salinity stress decreased growth parameters, increased malondialdehyde (MDA) contents and antioxidant enzymes such as superoxide dismutase (SOD), guaiacol peroxidase (POD) and catalase activities. Application of lower concentration of AHL had a relieving effect on Arabidopsis seedlings under salinity stress which dramatically decreased MDA content, and increased growth parameters as well as SOD and POD activities. Total proteins were extracted from the control, NaCl-, AHL- and NaCl + AHL-treated seedlings and were separated using two-dimensional gel electrophoresis. A total of 127 protein spots showed different expression compared with the control. Mass spectrometry analysis allowed the identification of 97 proteins involved in multiple pathways, i.e. defense/stress/detoxification, photosynthesis, protein metabolism, signal transduction, transcription, cell wall biogenesis, metabolisms of carbon, lipid, energy, sulfur, nucleotide and sugar. These results suggest that defense/stress response, metabolism and energy, signal transduction and regulation, protein metabolism and transcription-related proteins may be particularly subjected to regulation in salt stressed Arabidopsis seedlings, when treated with AHL and that this regulation lead to improved salt tolerance and plant growth. Overall, this study provides insight to the effect of AHL on salinity stress for the first time, and also sheds light on overview of the molecular mechanism of AHL-regulated plant growth promotion and salt resistance.

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Section: Introductionmentioning

confidence: 99%

Retracted: Proteomic and physiological responses of Arabidopsis thaliana exposed to salinity stress and N‐acyl‐homoserine lactone

Ding

Cao

Duan

et al. 2016

Physiologia Plantarum

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“…This suggested the possibility that TU supplementation could accelerate Na + translocation towards shoots which is considered as one of the important defence mechanism to avoid built up of Na + toxicity in roots43. Recently, comparative evaluation of contrasting rice varieties have confirmed that tolerant varieties have better potential of translocating Na + from root-to-shoot44. Although, no significant change in K + was observed in shoot; in roots, it was maintained at significantly higher level of 1.42−, 2.13− and 2-fold in NaCl, NaCl + TU and TU treatments, respectively, as compared with control (Fig.…”

Section: Resultsmentioning

confidence: 99%

Thiourea priming enhances salt tolerance through co-ordinated regulation of microRNAs and hormones in Brassica juncea

Srivastava

Sablok

Hackenberg

et al. 2017

Sci Rep

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Activation of stress tolerance mechanisms demands transcriptional reprogramming. Salt stress, a major threat to plant growth, enhances ROS production and affects transcription through modulation of miRNAs and hormones. The present study delineates salt stress ameliorating action of thiourea (TU, a ROS scavenger) in Brassica juncea and provides mechanistic link between redox, microRNA and hormones. The ameliorative potential of TU towards NaCl stress was related with its ability to decrease ROS accumulation in roots and increase Na+ accumulation in shoots. Small RNA sequencing revealed enrichment of down-regulated miRNAs in NaCl + TU treated roots, indicating transcriptional activation. Ranking analysis identified three key genes including BRX4, CBL10 and PHO1, showing inverse relationship with corresponding miRNA expression, which were responsible for TU mediated stress mitigation. Additionally, ABA level was consistently higher till 24 h in NaCl, while NaCl + TU treated roots showed only transient increase at 4 h suggesting an effective stress management. Jasmonate and auxin levels were also increased, which prioritized defence and facilitated root growth, respectively. Thus, the study highlights redox as one of the “core” components regulating miRNA and hormone levels, and also strengthens the use of TU as a redox priming agent for imparting crop resilience to salt stress.

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“…High-throughput omic techniques have been used to investigate complex molecular response underlying salt tolerance in crops [24, 26–31]. However, no related study has been done to reveal the genotypic differences of wild barleys in molecular responses to salt stress.…”

Section: Discussionmentioning

confidence: 99%

Multi-omics analysis reveals molecular mechanisms of shoot adaption to salt stress in Tibetan wild barley

Shen

Dai

et al. 2016

BMC Genomics

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BackgroundTibetan wild barley (Hordeum spontaneum L.) has been confirmed to contain elite accessions in tolerance to abiotic stresses, including salinity. However, molecular mechanisms underlying genotypic difference of salt tolerance in wild barley are unknown.ResultsIn this study, two Tibetan wild barley accessions (XZ26 and XZ169), differing greatly in salt tolerance, were used to determine changes of ionomic, metabolomic and proteomic profiles in the shoots exposed to salt stress at seedling stage. Compared with XZ169, XZ26 showed better shoot growth and less Na accumulation after 7 days treatments. Salt stress caused significant reduction in concentrations of sucrose and metabolites involved in glycolysis pathway in XZ169, and elevated level of tricarboxylic acid (TCA) cycle, as reflected by up-accumulation of citric acid, aconitic acid and succinic acid, especially under high salinity, but not in XZ26. Correspondingly, proteomic analysis further proved the findings from the metabolomic study.ConclusionXZ26 maintained a lower Na concentration in the shoots and developed superior shoot adaptive strategies to salt stress. The current result provides possible utilization of Tibetan wild barley in developing barley cultivars for salt tolerance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3242-9) contains supplementary material, which is available to authorized users.

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Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling

Abstract: HighlightComprehensive analyses of phenotypic, metabolic, and transcriptome data from two genotypes with contrasting salt tolerance provided a more complete picture of the molecular mechanisms underlying seedling tolerance in rice.

Cited by 118 publications

References 48 publications

Retracted: Proteomic and physiological responses of Arabidopsis thaliana exposed to salinity stress and N‐acyl‐homoserine lactone

Retracted: Proteomic and physiological responses of Arabidopsis thaliana exposed to salinity stress and N‐acyl‐homoserine lactone

Thiourea priming enhances salt tolerance through co-ordinated regulation of microRNAs and hormones in Brassica juncea

Multi-omics analysis reveals molecular mechanisms of shoot adaption to salt stress in Tibetan wild barley

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