Abstract-Automatic Music Transcription (AMT) can be performed by deriving a pitch-time representation through decomposition of a spectrogram with a dictionary of pitch-labelled atoms. Typically, Non-negative Matrix Factorisation (NMF) methods are used to decompose magnitude spectrograms. One atom is often used to represent each note. However, the spectrum of a note may change over time. Previous research considered this variability using different atoms to model specific parts of a note, or large dictionaries comprised of datapoints from the spectrograms of full notes. In this paper the use of subspace modelling of note spectra is explored, with group sparsity employed as a means of coupling activations of related atoms into a pitched subspace.Stepwise and gradient-based methods for non-negative group sparse decompositions are proposed. Finally, a group sparse NMF approach is used to tune a generic harmonic subspace dictionary, leading to improved NMF-based AMT results.