Complex of C60 Fullerene with Doxorubicin as a Promising Agent in Antitumor Therapy

Abstract

The main aim of this work was to evaluate the effect of doxorubicin in complex with C60 fullerene (C60 + Dox) on the growth and metastasis of Lewis lung carcinoma in mice and to perform a primary screening of the potential mechanisms of C60 + Dox complex action. We found that volume of tumor from mice treated with the C60 + Dox complex was 1.4 times less than that in control untreated animals. The number of metastatic foci in lungs of animals treated with C60 + Dox complex was two times less than that in control untreated animals. Western blot analysis of tumor lysates revealed a significant decrease in the level of heat-shock protein 70 in animals treated with C60 + Dox complex. Moreover, the treatment of tumor-bearing mice was accompanied by the increase of cytotoxic activity of immune cells. Thus, the potential mechanisms of antitumor effect of C60 + Dox complex include both its direct action on tumor cells by inducing cell death and increasing of stress sensitivity and an immunomodulating effect. The obtained results provide a scientific basis for further application of C60 + Dox nanocomplexes as treatment agents in cancer chemotherapy.

Determination of the equilibrium constant of C60 fullerene binding with drug molecules

“…However, during the past five years the complexation of pristine C60 fullerene with small drug molecules in aqueous solution has become a major focus of scientific investigation. In part, this is due to recent successes in combining co-administered C60 fullerene with antitumor drugs, resulting in improvement of medico-biological effects both in vivo and in vitro [12][13][14][15].…”

Biotransport kinetics and intratumoral biodistribution of malonodiserinolamide-derivatized [60]fullerene in a murine model of breast adenocarcinoma

“…For .25 years, derivatives of this class of NP have served as a platform for a variety of biomedical applications including X-ray and magnetic resonance imaging (MRI) contrast agents, [3][4][5][6] drug delivery, [5][6][7][8][9][10][11][12][13] antioxidant neuroprotection,…”

C60 fullerene enhances cisplatin anticancer activity and overcomes tumor cell drug resistance

“…For addressing these impediments, novel complex of the classical and highly active anticancer drug Cis with FC 60 was prepared and analyzed. Previous studies on FC 60 demonstrated its good biocompatibility and potential suitability to be used as delivery platform for another golden chemotherapy standard -namely Dox [29,30].…”

“…Recently we have demonstrated that anticancer drugs with anaromatic core may form non-covalent complexes with FC 60 in physiological solution [26,27], which was suggested to be a key process leading to amplification of the antitumor effect of antibiotic doxorubicin (Dox) in vitro towards various tumor lines and in vivo towards C57BL/6J male mice bearing Lewis lung carcinoma [28][29][30]. Based on these results, we hypothesized that the anticancer effects of other drugs may also be enhanced by their immobilization on FC 60 [27,31], and selected Cis as a potential candidate for conjugation on this novel nanocarrier.…”