Complex structure of bacillus subtilis RibG reduction mechanism in riboflavin biosynthesis

Chen, S.C.; Lin, Yu-Hsin; Yu, H.C.; Liaw, Shwu-Jen

doi:10.2210/pdb3ex8/pdb

2008

DOI: 10.2210/pdb3ex8/pdb

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Complex structure of bacillus subtilis RibG reduction mechanism in riboflavin biosynthesis

S.C. Chen¹,

Yu-Hsin Lin²,

H.C. Yu³

et al.

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Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

et al. 2021

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Background Knowledge on the localization and mobility of enzymes inside bacterial cells is scarce, but important for understanding spatial regulation of metabolism. The four central enzymes (Rib enzymes) of the riboflavin (RF) biosynthesis pathway in the Gram positive model bacterium Bacillus subtilis have been studied extensively in vitro, especially the heavy RF synthase, a large protein complex with a capsid structure formed by RibH and an encapsulated RibE homotrimer, which mediates substrate-channeling. However, little is known about the behavior and mobility of these enzymes in vivo. Results We have investigated the localization and diffusion of the Rib enzymes in the cytoplasm of B. subtilis. By characterizing the diffusion of Rib enzymes in live cells using single particle tracking (SPT) we provide evidence for confined diffusion at the cell poles and otherwise Brownian motion. A majority of RibH particles showed clear nucleoid occlusion and a high degree of confined motion, which is largely abolished after treatment with Rifampicin, revealing that confinement is dependent on active transcription. Contrarily, RibE is mostly diffusive within the cell, showing only 14% encapsulation by RibH nanocompartments. By localizing different diffusive populations within single cells, we find that fast diffusion occurs mostly across the nucleoids located in the cell centers, while the slower, confined subdiffusion occurs at the crowded cell poles. Conclusions Our results provide evidence for locally different motion of active enzymes within the bacterial cytoplasm, setting up metabolic compartmentalization mostly at the poles of cells.

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Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

et al. 2021

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Complex structure of bacillus subtilis RibG reduction mechanism in riboflavin biosynthesis

Cited by 1 publication

References 0 publications

Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

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